Plant Cell, Tissue and Organ Culture

, Volume 85, Issue 1, pp 53–61 | Cite as

Mycorrhized Ri-transformed roots facilitate in vitro inoculation of Cistus incanus with Tuber melanosporum

  • Yvonne Ventura
  • David Mills
  • Varda Kagan-Zur
  • Nurit Roth-Bejerano
  • Amnon  Bustan
Article
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Abstract

Progress was made towards a reliable in vitro system for mycorrhizing Cistus incanus seedlings with Tuber melanosporum. A rich growth medium favored extensive growth of mycorrhized Ri-transformed roots (MTR) but inhibited mycelial outgrowth into the medium. A minimal medium, on the other hand, inhibited MTR growth but supported considerable mycelial outgrowth into the medium. While the presence of a C.␣incanus propagule clearly enhanced mycelial growth into the minimal medium, a highly significant factor appeared to be the use of MTR inoculant, which supported mycorrhizal development to the Hartig net stage. The advantages of MTR for in vitro mycorrhization of host plant seedlings are discussed.

Keywords

Black Périgord truffle hairy roots inoculation method mycorrhiza 

Abbreviations

DDW

double distilled water

MTR

mycorrhized transformed roots

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • Yvonne Ventura
    • 1
  • David Mills
    • 1
  • Varda Kagan-Zur
    • 1
  • Nurit Roth-Bejerano
    • 1
  • Amnon  Bustan
    • 1
  1. 1.Institues for Applied Biology and AgricultureBen-Gurion University of the NegevBeer-ShevaIsrael

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