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Plant Cell, Tissue and Organ Culture

, Volume 84, Issue 2, pp 199–211 | Cite as

Agrobacterium-mediated transformation and inbred line development in the model grass Brachypodium distachyon

  • John P. Vogel
  • David F. Garvin
  • Oymon M. Leong
  • Daniel M. Hayden
Article

Abstract

Brachypodium distachyon (Brachypodium) has been proposed as a model temperate grass because its physical, genetic, and genome attributes (small stature, simple growth requirements, small genome size, availability of diploid ecotypes, annual lifecycle and self fertility) are suitable for a model plant system. Two additional requirements that are necessary before Brachypodium can be widely accepted as a model system are an efficient transformation system and homogeneous inbred reference genotypes. Here we describe the development of inbred lines from 27 accessions of Brachypodium. Determination of c-values indicated that five of the source accessions were diploid. These diploid lines exhibit variation for a variety of morphological traits. Conditions were identified that allow generation times as fast as two months in the diploids. An Agrobacterium-mediated transformation protocol was developed and used to successfully transform 10 of the 19 lines tested with efficiencies ranging from 0.4% to 15%. The diploid accession Bd21 was readily transformed. Segregation of transgenes in the T 1 generation indicated that most of the lines contained an insertion at a single genetic locus. The new resources and methodologies reported here will advance the development and utilization of Brachypodium as a new model system for grass genomics.

Keywords

c-value embryogenic callus genome size model system tissue culture 

Abbreviations

BA

benzylaminopurine

2,4-D

2,4-dichlorophenoxyacetic acid

LS

Linsmaier and Skoog basal medium

MS

Murashige and Skoog salts and vitamins

CIM

callus inducing medium

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Copyright information

© Springer 2006

Authors and Affiliations

  • John P. Vogel
    • 1
  • David F. Garvin
    • 2
  • Oymon M. Leong
    • 1
  • Daniel M. Hayden
    • 1
  1. 1.USDA Western Regional Research CenterAlbanyUSA
  2. 2.USDA-ARS Plant Science Research Unit and Department of Agronomy and Plant GeneticsUniversity of MinnesotaSt. PaulUSA

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