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Plant Cell, Tissue and Organ Culture

, Volume 84, Issue 2, pp 189–198 | Cite as

In vitro regeneration of Alstroemeria cv. ‘Yellow King’ by direct organogenesis

  • Martha E. Pedraza-Santos
  • Ma. Cristina López-Peralta
  • Víctor A. González- Hernández
  • E. Mark Engleman-Clark
  • Prometeo Sánchez-García
Article

Abstract

The common techniques for the in vitro production of Alstroemeria plants are based on rhizomes as explants, which have low multiplication rates and a high risk of carrying viral diseases. To overcome these problems, we developed a protocol for the in vitro regeneration of Alstroemeria cv.‘Yellow King’, by testing for shoot induction several explant sources (leaf, stem apices, rhizomes and immature inflorescence apices), temperature and light/dark regimes, hormone and salt concentrations. For shoot multiplication and rooting, several hormone concentrations were tested. We found that only the young floral apices produced adventitious shoots by direct organogenesis. The highest shoot induction rate (10.4 shoots per explant) was obtained by incubation in the dark for 15 days at 8 °C followed by 15 days at 25 °C and a 16-h/8-h light/dark regime, on a Murashige and Skoog (1962) liquid medium at 50% of the salt concentration, supplemented with 2.5 mg l−1 KIN, 1.5 mg l−1 BA and 1.0 mg l−1 NAA, using a piece filter paper to support the explant. The highest shoot multiplication rate (9 shoots per explant) was obtained on a liquid MS medium at full strength supplemented only with BA at 1.0 mg l−1. In vitro rooting of shoots was induced also on a liquid MS medium, either with or without plant hormones.

Keywords

Alstroemeria hormones light organogenesis salt concentration temperature 

Abbreviations

BA

6-benzyladenine

IAA

indole−3-acetic acid

KIN

kinetin

NAA

naphthaleneacetic acid

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References

  1. Bhattacharya P, Dey S, Bhattacharya BC, (1994) Use of low cost gelling agents and support matrices for industrial scale plant tissue culture Plant Cell Tiss. Org. Cult. 37: 15–23CrossRefGoogle Scholar
  2. Bond S, Alderson PG, (1993a) Establishment and growth of the rhizome of Alstroemeria as affected by temperature and the root system J. Hortic. Sci. 68(6): 847–853Google Scholar
  3. Bond S, Alderson PG, (1993b) The effect of explant density, temperature and light on rhizome growth in vitro of Alstroemeria J. Hortic. Sci. 68(6): 855–859Google Scholar
  4. Bridgen MP (1997) Alstroemeria. In: George J (ed) The Ball Red Book. 16th edn. (pp 341–348). Ball Publishing Company, West Chicago, ILGoogle Scholar
  5. Chiari A, Bridgen MP, (2000) Rhizome splitting: a new micropropagation technique to increase in vitro propagule Plant Cell Tiss. Org. Cult. 62: 39–46CrossRefGoogle Scholar
  6. Debergh PY, (1982) Physical properties of culture media. In: Fujiwara A, (ed). Proceedings of the 5th International Congress of Plant Tissue and Cell Culture. Tokyo, pp. 135–136Google Scholar
  7. Declerck V, Korban S, (1994) Effects of source of macronutrients and plant growth regulator concentrations on shoot proliferation of Cornus florida Plant Cell. Tiss. Org. Cult. 38: 57–60CrossRefGoogle Scholar
  8. Gabryszewska E, Hempel M, (1985) The influence of cytokinins and auxins on Alstroemeria in tissue culture Acta Hortic. 167: 295–300Google Scholar
  9. George EF (1993) Plant Propagation by Tissue Culture. Part I. The Technology. Chapter 7. Factors Affecting Growth and Morphogenesis. I. Genotype and the Physical Environment, 2nd edn. (574 p.). Exegetics Limited Edington, LondonGoogle Scholar
  10. George EF, Sherrington PD, (1984) Plant Propagation by Tissue Culture Exegetics Limited. Basingstoke, pp. 709Google Scholar
  11. Hakkaart FA, Versluijs JMA, (1988) Virus elimination by meristem-tip culture from a range of Alstroemeria cultivars Neth. J. Plant Pathol. 94: 49–56CrossRefGoogle Scholar
  12. Hutchinson MJ, Senaratna T, Tsujita JM, Saxena PK, (1997) Somatic embryogenesis in liquid cultures of a tetraploid Alstroemeria Plant Cell Tiss. Org. Cult. 47: 293–297CrossRefGoogle Scholar
  13. Kristiansen K, Ornstrup H, Brandt K, (1999) In vitro PPFD and media composition affect both in and ex vitro performance of Alstroemeria Butterfly-hybrids Plant Cell Tiss. Org. Cult. 56: 145–153CrossRefGoogle Scholar
  14. Lin HS, De Jeu MJ, Jacobsen E, (1997) Direct shoot regeneration form excised leaf explants of in vitro grown seedlings of Alstroemeria L Plant Cell Rep. 16: 770–774CrossRefGoogle Scholar
  15. Lin HS, De Jeu MJ, Jacobsen E, (1998) Formation of shoots from leaf axils of Alstroemeria: the effect of the position on the stem Plant Cell Tiss. Org. Cult. 52: 165–169CrossRefGoogle Scholar
  16. Lin HS, De Jeu MJ, Jacobsen E, (2000) The application of leafy explant micropropagation protocol in enhancing the multiplication efficiency of Alstroemeria Sci. Hortic. 85: 307–318CrossRefGoogle Scholar
  17. Lin WC, Monette PL, (1987) In vitro propagation of Alstroemeria ‘Alsaan’ Plant Cell Tiss. Org. Cult. 9: 29–35CrossRefGoogle Scholar
  18. Morard P, Henry M, (1998) Optimization of the mineral composition of in vitro culture media J. Plant Nutr. 21(8): 1565- 1576CrossRefGoogle Scholar
  19. Murashige T, Skoog F, (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant. 15: 473–497CrossRefGoogle Scholar
  20. Nitsch JP, Nitsch C, (1969) Haploid plants from pollen grains Science 163: 85–87PubMedCrossRefGoogle Scholar
  21. Pedersen C, Brandt K, (1992) A method for disinfection of underground rhizome tips of Alstroemeria and Heliconia Acta Hortic. 325: 499–504Google Scholar
  22. Pedersen C, Hansen CW, Brandt K, Kristiansen K, (1996). Alstroemeria plantlets can be induced to flowering by cold treatment during in vitro culture Sci. Hortic. 66: 217–228CrossRefGoogle Scholar
  23. Pierik RLM (1990) Cultivo in vitro de las Plantas Superiores (p. 326). Mundi-Prensa. España, MadridGoogle Scholar
  24. Pierik RLM, Van Voorst A, Booy G, Van Acker CAM, Lelivelt CLC, de Wit JC, (1988) Vegetative propagation of Alstroemeria in vitro Acta Hortic. 226: 81–89Google Scholar
  25. Podwysznska M, Gabryszewska E, Przybyla A, (1997) Micropropagation of Alstroemeria x hybrida ‘Juanita’ Acta Hortic. 447: 175–177Google Scholar
  26. Sánchez GP, Martínez BN, (1999) Nutrición Mineral de Alstroemeria Publicación especial No. 9 Sociedad Mexicana de la Ciencia del Suelo, México, p. 33Google Scholar
  27. SAS Institute 1997. SAS/STAT User’s Guide Release 6.03 Edn, Cary, NC, USAGoogle Scholar
  28. Van Zaayen, A, (1995) Alstroemeria. In: Loebenstein G, Lawson RH, Brunt AA, (eds.). Virus and Virus-like Diseases of Bulbs and Flower Crops. Wiley Publisher, Chichester, UK., pp. 237–249Google Scholar
  29. White PR, (1963) The Cultivation of Animal and Plant Cells. 2. Ronald Press. New York, NYGoogle Scholar
  30. Ziv M, Kanterovitz R, Halevy AH, (1973) Vegetative propagation of Alstroemeria in vitro Sci. Hortic. 1:271–277CrossRefGoogle Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Martha E. Pedraza-Santos
    • 1
  • Ma. Cristina López-Peralta
    • 1
  • Víctor A. González- Hernández
    • 1
  • E. Mark Engleman-Clark
    • 2
  • Prometeo Sánchez-García
    • 3
  1. 1.Programas en Fisiología Vegetal, Instituto de Recursos Genéticos y ProductividadColegio de PostgraduadosMontecilloEstado de México
  2. 2.Botánica, Instituto de Recursos Genéticos y ProductividadColegio de PostgraduadosMontecilloEstado de México
  3. 3.Edafología, Instituto de Recursos NaturalesColegio de PostgraduadosMontecilloEstado de México

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