Plant Cell, Tissue and Organ Culture

, Volume 84, Issue 2, pp 189–198 | Cite as

In vitro regeneration of Alstroemeria cv. ‘Yellow King’ by direct organogenesis

  • Martha E. Pedraza-Santos
  • Ma. Cristina López-Peralta
  • Víctor A. González- Hernández
  • E. Mark Engleman-Clark
  • Prometeo Sánchez-García


The common techniques for the in vitro production of Alstroemeria plants are based on rhizomes as explants, which have low multiplication rates and a high risk of carrying viral diseases. To overcome these problems, we developed a protocol for the in vitro regeneration of Alstroemeria cv.‘Yellow King’, by testing for shoot induction several explant sources (leaf, stem apices, rhizomes and immature inflorescence apices), temperature and light/dark regimes, hormone and salt concentrations. For shoot multiplication and rooting, several hormone concentrations were tested. We found that only the young floral apices produced adventitious shoots by direct organogenesis. The highest shoot induction rate (10.4 shoots per explant) was obtained by incubation in the dark for 15 days at 8 °C followed by 15 days at 25 °C and a 16-h/8-h light/dark regime, on a Murashige and Skoog (1962) liquid medium at 50% of the salt concentration, supplemented with 2.5 mg l−1 KIN, 1.5 mg l−1 BA and 1.0 mg l−1 NAA, using a piece filter paper to support the explant. The highest shoot multiplication rate (9 shoots per explant) was obtained on a liquid MS medium at full strength supplemented only with BA at 1.0 mg l−1. In vitro rooting of shoots was induced also on a liquid MS medium, either with or without plant hormones.


Alstroemeria hormones light organogenesis salt concentration temperature 





indole−3-acetic acid




naphthaleneacetic acid


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Copyright information

© Springer 2005

Authors and Affiliations

  • Martha E. Pedraza-Santos
    • 1
  • Ma. Cristina López-Peralta
    • 1
  • Víctor A. González- Hernández
    • 1
  • E. Mark Engleman-Clark
    • 2
  • Prometeo Sánchez-García
    • 3
  1. 1.Programas en Fisiología Vegetal, Instituto de Recursos Genéticos y ProductividadColegio de PostgraduadosMontecilloEstado de México
  2. 2.Botánica, Instituto de Recursos Genéticos y ProductividadColegio de PostgraduadosMontecilloEstado de México
  3. 3.Edafología, Instituto de Recursos NaturalesColegio de PostgraduadosMontecilloEstado de México

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