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Plant Cell, Tissue and Organ Culture

, Volume 84, Issue 2, pp 181–188 | Cite as

Regeneration of Syngonium podophyllum ‘Variegatum’ through direct somatic embryogenesis

  • Qian Zhang
  • Jianjun Chen
  • Richard J. Henny
Article

Abstract

A simple and effective method of regenerating Syngonium podophyllum ‘Variegatum’ via direct somatic embryogenesis has been established. Leaf and petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU) or N-phenyl-N′-1,2,3-thiadiazol-5-ylurea (TDZ) with either α-naphthalene acetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos directly formed at one or two sides of petiole explants on MS medium supplemented 2.5 mg l−1 TDZ with 0.5 mg l−1 NAA or 2.0 mg l−1 TDZ with 0.2 mg l−1 NAA or with 0.2 and 0.5 mg l−1 2,4-D, respectively. The frequency of petiole explants with somatic embryos produced was as high as 86% when cultured on medium containing 2.5 mg l−1 TDZ with 0.5 mg l−1 NAA. Up to 85% of somatic embryos were able to germinate after transferring onto medium containing 2.0 mg l−1 6-benzylaminopurine (BA) and 0.2 mg l−1 NAA. Approximately 50–150 plantlets were regenerated from a single petiole explant. However, there was no somatic embryo formation from leaf explants regardless of growth regulator combinations used. Regenerated plantlets from petiole explants were stable and grew vigorously after transplanting to a soilless container substrate in a shaded greenhouse.

Keywords

goosefoot plant nephthytis micropropagation tropical ornamental foliage plants 

Abbreviations

BA

6-benzylaminopurine

CPPU

N-(2-chloro-4-pyridyl)-N-phenylurea

2,4-D

2,4-dichlorophenoxyacetic acid

MES

(2-(N-morpholino) ethane-sulfonic acid

MS

Murashige and Skoog’s medium

NAA

α-naphthalene acetic acid

TDZ

N-phenyl-N′-1,2,3-thiadiazol-5-ylurea

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Notes

Acknowledgements

This research was supported by the Florida Agricultural Experiment Station and approved for publication as journal series no. R-10848.

References

  1. Ahloowalia BS, (1991) Somatic embryos in monocots: their genesis and genetic stability Rev. Cytol. Biol. Veget-Bot 14:223–235Google Scholar
  2. Bown D, (2000) Aroids: Plants of the Arum Family 2nd Ed. Timber Press, Inc. Portland, ORGoogle Scholar
  3. Chase AR, (1997) Foliage Plant Diseases: Diagnosis and Control APS Press St. Paul, MNGoogle Scholar
  4. Chen J, Henny RJ, McConnell DB, (2002) Development of new foliage plant cultivars. In: Janick J, Whipkey A, (eds) Trends in New Crops and New Uses. ASHS Press; Alexandria, VA, pp. 466–472Google Scholar
  5. Chen J, Henny RJ, Chao TC (2003) Somaclonal variation as a source for cultivar development of ornamental aroids. In: Pandalai SG (ed) Recent Research Development in Plant Science, Vol. 1 (pp. 31–43). Signpost Kerala, India Google Scholar
  6. Chen J, McConnell DB, Henny RJ, Norman DJ, (2005) The foliage plant industry Hortic. Rev. 31:47–112Google Scholar
  7. Croat TB, (1982) A revision of Syngonium (Araceae) Ann. Missouri Bot. Gard. 68:565–651CrossRefGoogle Scholar
  8. Encheva J, Kohler H, Friedt W, Tsvetkova F, Ivanov P, Encheva V, Shindrova P, (2003) Field evaluation of somaclonal variation in sunflower (Helianthus annuus L.) and its application for crop improvement Euphytica 130:167–175CrossRefGoogle Scholar
  9. Fowler MR, Ong LM, Russinova E, Atanassov AI, Scott NW, Slater A, Elliott MC, (1998) Early changes in gene expression during direct somatic embryogenesis in alfalfa revealed by RAP-PCR J. Exp. Bot. 49:249–253Google Scholar
  10. Gaj MD, (2001) Direct somatic embryogenesis as a rapid and efficient system for in vitro regeneration of Arabidopsis thaliana Plant Cell Tissue Organ Cult. 64:39–46CrossRefGoogle Scholar
  11. Griffith LP, (1998) Tropical Foliage Plants: A Grower’s Guide Ball Pub Batavia, ILGoogle Scholar
  12. Henley RW, Robinson CA, (1993) Nephthytis cultivars to know and grow Proc. Fla. State Hort. Soc 106:343–347Google Scholar
  13. Henny RJ, Chen J, (2003) Foliage plant cultivar development Plant Breeding Rev. 23:245–290Google Scholar
  14. Huetteman CA, Preece JE, (1993) Thidiazuron: a potent cytokinin for plant tissue culture Plant Cell Tissue organ Cult 33:105–119CrossRefGoogle Scholar
  15. Kane MK, (2000) Micropropagation of Syngonium by shoot culture. In: Trigiano RN, Gray DJ, (eds) Plant Tissue Culture Concepts and Laboratory Exercise. CRC Press, Boca Raton, FL, pp. 87–95Google Scholar
  16. Kintzios S, Manos C, Makri O, (1999) Somatic embryogenesis from mature leaves of rose (Rosa sp.) Plant Cell Rep. 18:467–472CrossRefGoogle Scholar
  17. Larkin PJ, Scowroft WR, (1981) Somaclonal variation: a novel source of variation from cell culture for plant improvement Theor. Appl. Genet. 60:197–214CrossRefGoogle Scholar
  18. Levin R, Stav R, Alper Y, Watad AA, (1996) In vitro multiplication in liquid culture of Syngonium contaminated with Bacillus spp. and Rathayibacter tritici Plant Cell Tissue Organ Cult. 45:277–280CrossRefGoogle Scholar
  19. Mayo SJ, Bogner J, Boyce PC, (1997) The Genera of Araceae. Royal Bot Gardens Kew, UKGoogle Scholar
  20. Merkle SA, (1997) Somatic embryogenesis in ornamentals. In: Geneve RL, Preece JE, Merkle SA, (eds) Biotechnology of Ornamental Plants. CAB Intl Wallingford, UK pp. 13–33Google Scholar
  21. Miller LR, Murashige T, (1976) Tissue culture propagation of tropical foliage plants In Vitro 12:797–813PubMedCrossRefGoogle Scholar
  22. Murashige T, Skoog F, (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant 15:473–479CrossRefGoogle Scholar
  23. Norman DJ, Henny RJ, Yuen JMF & Mellich TA, (2003) Screening for resistance to myrothecium leaf spot among Syngonium species and cultivars HortScience 38:75–76Google Scholar
  24. Rani V, Raina SN, (2000) Genetic fidelity of organized meristem-derived micropropagated plants: a critical reappraisal In Vitro Cell Dev. Bio.-Plant 36:319–330CrossRefGoogle Scholar
  25. Shibli RA, Shatnawi M, Abu-Ein & Al-Juboory KH, (2001) Somatic embryogenesis and plant recovery from callus of ‘Nabali’ olive (Olea europea L.) Sci. Hortic 88:243–256CrossRefGoogle Scholar
  26. Skirvin RM, McPheeters KD, Norton M, (1994) Sources and frequency of somaclonal variation HortScience 29:1232–1237Google Scholar
  27. Vasil IK, (1987) Developing cell and tissue culture systems for the improvement of cereal and grass crops J. Plant Physiol 128:193–218CrossRefGoogle Scholar
  28. Watad AA, Raghothama KG, Kochba M, Nissim A, Gaba V, (1997) Micropropagation of Spathiphyllum and Syngonium is facilitated by use of interfacial membrane rafts HortScience 32:307–308Google Scholar
  29. Zhang Q, Chen J, Henny RJ, (2005) Somatic embryogenesis and plant regeneration of leaf, petiole, and stem explants of Golden Pothos Plant Cell Rep. 23:587–595PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  1. 1.IFAS, Environmental Horticulture Department and Mid-Florida Research and Education CenterUniversity of FloridaApopkaUSA

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