Plant Cell, Tissue and Organ Culture

, Volume 84, Issue 2, pp 181–188 | Cite as

Regeneration of Syngonium podophyllum ‘Variegatum’ through direct somatic embryogenesis

  • Qian Zhang
  • Jianjun Chen
  • Richard J. Henny


A simple and effective method of regenerating Syngonium podophyllum ‘Variegatum’ via direct somatic embryogenesis has been established. Leaf and petiole explants were cultured on Murashige and Skoog (MS) medium supplemented with N-(2-chloro-4-pyridyl)-N′-phenylurea (CPPU) or N-phenyl-N′-1,2,3-thiadiazol-5-ylurea (TDZ) with either α-naphthalene acetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryos directly formed at one or two sides of petiole explants on MS medium supplemented 2.5 mg l−1 TDZ with 0.5 mg l−1 NAA or 2.0 mg l−1 TDZ with 0.2 mg l−1 NAA or with 0.2 and 0.5 mg l−1 2,4-D, respectively. The frequency of petiole explants with somatic embryos produced was as high as 86% when cultured on medium containing 2.5 mg l−1 TDZ with 0.5 mg l−1 NAA. Up to 85% of somatic embryos were able to germinate after transferring onto medium containing 2.0 mg l−1 6-benzylaminopurine (BA) and 0.2 mg l−1 NAA. Approximately 50–150 plantlets were regenerated from a single petiole explant. However, there was no somatic embryo formation from leaf explants regardless of growth regulator combinations used. Regenerated plantlets from petiole explants were stable and grew vigorously after transplanting to a soilless container substrate in a shaded greenhouse.


goosefoot plant nephthytis micropropagation tropical ornamental foliage plants 







2,4-dichlorophenoxyacetic acid


(2-(N-morpholino) ethane-sulfonic acid


Murashige and Skoog’s medium


α-naphthalene acetic acid




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This research was supported by the Florida Agricultural Experiment Station and approved for publication as journal series no. R-10848.


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Copyright information

© Springer Science+Business Media, Inc. 2005

Authors and Affiliations

  1. 1.IFAS, Environmental Horticulture Department and Mid-Florida Research and Education CenterUniversity of FloridaApopkaUSA

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