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Shoot multiplication in cultures of mature Alnus glutinosa


Shoot cultures were initiated from mature trees of Alnus glutinosa. On medium containing 1–5 μM 6-benzylamino purine (BAP), the shoots elongated without branching, formed heavy callus at the base of the stems and readily formed roots. The possibility that these characteristics could be attributed to the strong influence of endogenous auxin was tested on media that contained two auxin transport inhibitors, 1-N- naphthylphthalamic acid (NPA) and 2,3,5-triiodobenzoic acid (TIBA), at concentrations of 0.1–3 μM, in combination with 2 μM BAP. On these media, shoots produced numerous branches, less callus and no roots. After 30 weeks (five subcultures) on this medium, leaves were smaller and showed signs of vitrification. These problems were resolved without detriment to shoot proliferation, by reverting to medium without NPA or TIBA. Shoots rooted readily after transfer to medium without growth regulators and were successfully acclimatised after transfer to soil.

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  1. Benson DR & Silvester WB (1993) Biology of Frankia strains, actinomycete symbionts of actinorhizal plants. Microbiol. Rev. June 1993: 293–319

  2. T Christopher MV Rajam (1994) ArticleTitleIn vitro clonal propagation of Capsicum spp Plant Cell Tiss. Org. Cult. 38 25–29 Occurrence Handle10.1007/BF00034439

  3. S Garton MA Hosier PE Read RS Farnham (1981) ArticleTitleIn vitro propagation of Alnus glutinosa Gaertn Hort. Sci. 16 758–759

  4. CJ Gill (1975) ArticleTitleThe ecological significance of adventitious rooting as a response to flooding in woody species, with special reference to Alnus glutinosa Flora 164 85–97

  5. MS Greenwood (1987) ArticleTitleRejuvenation of forest trees Plant Growth Regul. 6 1–12 Occurrence Handle10.1007/BF00043947

  6. P Lakshmanan CL Lee CJ Goh (1997) ArticleTitleAn efficient in vitro method for mass propagation of a woody ornamental Ixora coccinea L Plant Cell Rep. 16 572–577

  7. TL Lomax GK Muday PH Rubery (1995) Auxin transport PJ Davies (Eds) Plant Hormones Kluwer Academic Publishers Netherlands 509–530

  8. TR Marks SE Simpson (1994) ArticleTitleFactors affecting shoot development in apically dominant Acer cultivars in vitro J. Hortic. Sci. 69 543–537

  9. BH McCown G Lloyd (1981) ArticleTitleWoody plant medium (WPM) – a mineral nutrient formulation for microculture of woody plant-species Hort. Sci. 16 453

  10. T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant. 15 473–497

  11. P Perinet M Lalonde (1983) ArticleTitleIn vitro propagation and nodulation of the actinorhizal host plant Alnus glutinosa (L.) Gaertn Plant Sci. Lett. 29 9–17 Occurrence Handle10.1016/0304-4211(83)90018-4

  12. RLM Pierik (1990) Rejuvenation and micropropagation HJJ Nijkamp LWH Plas ParticleVan der J Aartrijk ParticleVan (Eds) Progress in Plant Cellular and Molecular Biology Kluwer Academic Publishers Dordrecht, Netherlands 91–101

  13. RS Poething (1990) ArticleTitlePhase change and the regulation of shoot morphogenesis in plants Science 259 923–930

  14. PE Read R Sheldrake (1966) ArticleTitleCorrection of chlorosis of plants grown in Cornell peat-lite mixes Proc. Amer. Soc. Hortic. Sci. 88 576–581

  15. SK Singh MM Syamal (2000) ArticleTitleAnti-auxin enhance Rosa hybrida L. micropropagation Biol. Plant. 43 279–281 Occurrence Handle10.1023/A:1002768613872

  16. EF Smith AV Roberts J Mottley (1990) ArticleTitleThe preparation in vitro of chrysanthemum for transplantation to soil 3. Improved Resistance to desiccation conferred by reduced humidity Plant Cell Tiss. Org. Cult. 21 141–145 Occurrence Handle10.1007/BF00033433

  17. RR Sokal FJ Rolfe (1994) Biometry EditionNumber3 WH Freeman & Co New York

  18. FM Tremblay M Lalonde (1984) ArticleTitleRequirements for the in vitro propagation of seven nitrogen-fixing Alnus species Plant Cell Tiss. Org. Cult. 3 189–199 Occurrence Handle10.1007/BF00040337

  19. FM Tremblay P Perinet M Lalonde (1986) Tissue culture of Alnus spp. with regard to symbioses YPS Bajaj (Eds) Biotechnology in Agriculture and Forestry (Vol. 1 Trees 1) Springer-Verlag Berlin Heidelberg 87–100

  20. C Voyiatzi DG Voyiatzis V Tsiakmaki (1995) ArticleTitleIn vitro proliferation rates of the rose cv. (hybrid tea) ‘Dr. Verhage’, as affected by apical dominance regulating substances Sci. Hortic. 61 241–249 Occurrence Handle10.1016/0304-4238(94)00750-A

  21. M Welander NT Welander A-S Brackman (1989) ArticleTitleRegulation of in vitro shoot multiplication in Syringa, Alnus and Malus by different carbon sources J. Hortic. Sci. 64 361–366

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Correspondence to A. V. Roberts.

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Lall, S., Mandegaran, Z. & Roberts, A.V. Shoot multiplication in cultures of mature Alnus glutinosa. Plant Cell Tiss Organ Cult 83, 347–350 (2005).

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  • 1-N- naphthylphthalamic acid (NPA)
  • 2,3,5-triiodobenzoic acid (TIBA)
  • auxin transport inhibitors
  • mature trees
  • somatic embryos