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Selection of turmeric callus tolerant to culture filtrate of Pythium Graminicolum and regeneration of plants

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Abstract

Root rot disease tolerant clones of turmeric variety Suguna of Curcuma longa L. were isolated using continuous in vitro selection technique against pure culture filtrate of Pythium graminicolum. Large amount of profuse, compact, creamish white callus was obtained from in vivo vegetative bud when cultured on LSBM fortified with 2,4-D (3 mg l−1) after 45 days of culture. Callus was challenged with pure culture filtrate of P. graminicolum to isolate viable callus within 30 days of culture, which was further subjected to pure culture filtrate treatment. After three cycles of treatment, four cell lines which are tolerant to culture filtrate was isolated through continuous in vitro selection and subcultured on regeneration medium LSBM fortified with BAP (4 mg l−1) along with the control non-selected callus to obtain complete plantlets through discontinuous in vitro selection technique. Plants regenerated from tolerant and non-selected calli were screened for disease tolerance by adopting in vitro sick plot technique. The data obtained from this experiment revealed a ratio of 225:49 tolerant: susceptible in vitro clones retrieved from tolerant callus. However, plants regenerated from the CL1a1 and non-selected calli were susceptible under in vitro sick plot technique. The root rot disease tolerant clones were hardened and established in soil with 90% survival frequency.

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  1. Plant Biotechnology Unit, Annexe-Department of Botany, Bangalore University, Bangalore, Karnataka, 560 056, India

    M. C. Gayatri, V. Roopa darshini & R. Kavyashree

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  1. M. C. Gayatri
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  2. V. Roopa darshini
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  3. R. Kavyashree
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Correspondence to M. C. Gayatri.

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Gayatri, M.C., darshini, V.R. & Kavyashree, R. Selection of turmeric callus tolerant to culture filtrate of Pythium Graminicolum and regeneration of plants. Plant Cell Tiss Organ Cult 83, 33–40 (2005). https://doi.org/10.1007/s11240-005-3706-0

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