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Plant Cell, Tissue and Organ Culture

, Volume 83, Issue 1, pp 21–32 | Cite as

Genome fidelity during short- and long-term tissue culture and differentially cryostored meristems of silver birch (Betula pendula)

  • Leena Ryynänen
  • Tuija Aronen
Article

Abstract

Clonal trueness of micropropagated or cryopreserved material is essential, especially with long-living tree species. In this study, the growth rate and morphology of regenerated silver birch (Betula pendula Roth) plants growing in the nursery were evaluated after different treatments: short-term (14 months) and long-term (70 months) tissue culture periods, cryostorage of in vivo buds and cryopreservation of in vitro shoot apices using four different slow cooling cryopreservation protocols with PGD (10% PEG, 10% glucose, 10% DMSO) as cryoprotectant. Genetic fidelity of the regenerated plants compared to the original donor trees was evaluated using RAPD assays together with chromosome analysis. The regenerated plants showed no genetic or phenotypic changes, and can thus be considered as reliable material for any research, breeding or silvicultural activities.

Key words:

chromosomes cryopreservation deciduous tree genetic stability RAPDs tissue culture period 

Abbreviations

ABA

abscisic acid

BA

6-benzylaminopurine

DMSO

dimethylsulphoxide

LD

long day (16/8 h light/dark photoperiod)

PGD

10% polyethylene glycol (w/v), 10% glucose (w/v), and 10% DMSO (v/v) in water

RAPDs

random amplified polymorphic DNAs

SD

short day (8/16 h light/dark photoperiod)

WPM

woody plant medium

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Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Finnish Forest Research Institute, Punkaharju Research StationFinland

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