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Isolated microspore culture of Canadian 6× triticale cultivars


Isolated microspore culture was conducted on nine Canadian triticale cultivars (X Triticosecale Wittmack) using two induction media developed for wheat, with or without 100 g l−1 Ficoll. Significant interactions were observed for the number of embryos and calluses induced, green and albino plantlets regenerated and fertility of green plants. Ficoll was beneficial in both media to increase numbers of embryos and green plants for all cultivars. Overall, medium NPB99 supplemented with ficoll provided the most suitable condition for most cultivars. AC Alta performed slightly better on CHB3 supplemented with Ficoll. Only cv. Wapiti was not amenable to androgenesis. The cultivars AC Certa, AC Copia, AC Alta, Sandro, Ultima, Frank, Pronghorn and Banjo produced respectively, 10, 9, 6, 5, 4, 3, 3 and 1 green plants per Petri dish (35,000 microspores), on their optimum treatment. Twenty-two percent of total lines produced were fertile, and considered doubled haploids. The application of isolated microspore culture to triticale, opens new possibilities in breeding triticale, for the utilization of in vitro selection and genetic engineering.

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  1. A Arzani NL Darvey (2002) ArticleTitleAndrogenetic response of heterozygous triticale populations using a greenhouse hydroponic system Euphytica 127 53–60 Occurrence Handle10.1023/A:1019977304182

  2. B Barnabas E Szakacs I Karsai Z Bedo (2000) ArticleTitleIn vitro androgenesis of wheat: from fundamentals to practical application Euphytica 119 211–216 Occurrence Handle10.1023/A:1017558825810

  3. S Bernard E Picard JD Buyser (1976) ArticleTitleObtaining haploid plants from Triticale hexaploides (X Triticosecale Wittmack) by in vitro anther culture C. R. Acad. Sci. 283 235–238

  4. AR Carlson J Letarte J Chen KJ Kasha (2001) ArticleTitleVisual screening of microspore-derived transgenic barley (Hordeum vulgare L.) with green-fluorescent protein Plant Cell Rep. 20 331–337 Occurrence Handle10.1007/s002990100328

  5. G Charmet S Bernard (1984) ArticleTitleDiallel analysis of androgenetic plant production in hexaploid triticale (X TriticosecaleWittmack) Theor. Appl. Genet. 69 55–61 Occurrence Handle10.1007/BF00262539

  6. CC Chu RD Hill AL Brulé-Babel (1990) ArticleTitleHigh frequency of pollen embryoid formation and plant regeneration in Triticum aestivum L. on monosaccharide media Plant Sci. 66 255–262 Occurrence Handle10.1016/0168-9452(90)90211-6

  7. Darvey NL, Fazal JA, Barbera F, Ancora G, Rimes A (1991) A modified anther culture methodology for increasing embryoid production in wheat and triticale. In: Proceedings of the Second International Triticale Symposium, Passo Fundo, Rio Grande do Sul, Brazil 1–5 Oct. 1990 (pp. 314–319). CIMMYT, Mexico, DF.

  8. F Eudes S Acharya A Laroche LB Selinger KJ Cheng (2003) ArticleTitleA novel method to induce direct somatic embryogenesis, secondary embryogenesis and regeneration of fertile green cereal plants Plant Cell Tiss. Org. Cult. 73 147–157 Occurrence Handle10.1023/A:1022800512708

  9. M Gonzalez N Jouve (2000) ArticleTitleImprovement of anther culture media for haploid production in triticale Cereal Res. Commun. 28 65–72

  10. M Gonzalez I Hernandez N Jouve (1997) ArticleTitleAnalysis of anther culture response in hexaploid triticale Plant Breed. 116 302–304

  11. DS Hassawi GH Liang (1990) ArticleTitleEffect of cultivar, incubation temperature, and stage of microspore development on anther culture in wheat and triticale Plant Breed. 105 332–336

  12. T Hu KJ Kasha (1997) ArticleTitleImprovement of isolated microspore culture of wheat (Triticum aestivum L.) through ovary co-culture Plant Cell Rep. 16 520–252 Occurrence Handle10.1007/s002990050271

  13. S Immonen J Robinson (2000) ArticleTitleStress treatments and ficoll for improving green plant regeneration in triticale anther culture Plant Sci. 150 77–84 Occurrence Handle10.1016/S0168-9452(99)00169-7

  14. A Jähne H Lörz (1995) ArticleTitleCereal microspore culture Plant Sci. 109 1–12 Occurrence Handle10.1016/0168-9452(95)04149-O

  15. I Karsai Z Bedo PM Hayes (1994) ArticleTitleEffect of induction medium pH and maltose concentration on in vitro androgenesis of hexaploid winter triticale and wheat Plant Cell Tiss. Org. Cult. 39 49–53 Occurrence Handle10.1007/BF00037591

  16. J Keller (1991) ArticleTitleInfluence of media conditioning on in vitro development in pollen suspensions of triticale Biol. Zentralblatt 110 207–214

  17. C Lehmann KD Krolow (1991) ArticleTitleExperiments on haploid production from tetraploid triticales by the Hordeum bulbosum system and anther culture Cereal Res. Commun. 19 283–290

  18. SF Lukjanjuk SA Ignatova (1986) Triticale: production of haploid and homozygous plants YPS Bajaj (Eds) Biotechnology in Agriculture and Forestry, Vol.2 Springer-Verlag Berlin 530–543

  19. K Marciniak Z Banaszak M Wedzony (1998) ArticleTitleEffect of genotype, medium and sugar on triticale (X Triticosecale Wittm.) anther culture response Cereal Res. Commun. 26 145–151

  20. K Marciniak Z Kaczmarek T Adamski M Surma (2003) ArticleTitleThe anther-culture response of triticale line × tester progenies Cell. Mol. Biol. Lett. 8 343–351 Occurrence Handle12813569

  21. SN Matveenko LV Khotyleva LN Kaminskaya (1994) ArticleTitleGenetic analysis of efficiency of triticale anther culture in vitro Genetika 30 1238–1242

  22. T Monostori J Páuk M Puolimatka (1998) ArticleTitleTriticale (X Triticosecale Wittmack) in vitro androgenesis in isolated microspore culture Novenytermeles 47 371–382

  23. S Oleszczuk S Sowa J Zimny (2004) ArticleTitleDirect embryogenesis and green plant regeneration from isolated microspores of hexaploid triticale (X Triticosecale Wittmack) cv Bogo. Plant Cell Rep. 22 885–893

  24. H Ono EN Larter (1976) ArticleTitleAnther culture of triticale Crop Sci. 16 120–122

  25. J Páuk M Puolimatka KL Tóth T Monostori (2000) ArticleTitleIn vitro androgenesis of triticale in isolated microspore culture Plant Cell Tiss. Organ Cult. 61 221–229 Occurrence Handle10.1023/A:1006416116366

  26. W Pohler G Schumann M Sulze IM Kummer (1988) ArticleTitleCytological investigation of callus tissue and regenerated plants from triticale anther culture Biol. Zentralblatt 107 643–652

  27. A Ponitka A Slusarkiewicz Jarzina M Wedzony I Marcinska J Wozna (1999) ArticleTitleThe influence of various in vitro culture conditions on androgenetic embryo induction and plant regeneration from hexaploid triticale (X Triticosecale Wittm.). J Appl. Gen. 40 165–174

  28. PH Ryoppy (1996) Haploidy in triticale SM Jain SK Sopory RF Veilleux (Eds) vitro Haploid Production in Higher Plants, Vol.4 Kluwer Academic Publishers Dordrecht 117–131

  29. SAS Institute Inc. (1988) SAS/STAT User’s Guide: Release 6.03. SAS Institute Inc., Cary, p. 1029.

  30. A Slusarkiewicz Jarzina A Ponitka (2003) ArticleTitleEfficient production of spontaneous and induced doubled haploid triticale plants derived from anther culture Cereal Res. Commun. 31 289–296

  31. S Tuvesson A Ljungberg N Johansson KE Karlsson LW Suijs JP Josset (2000) ArticleTitleLarge-scale production of wheat and triticale double haploids through the use of a single-anther culture method Plant Breed. 119 455–459 Occurrence Handle10.1046/j.1439-0523.2000.00536.x

  32. YY Wang CS Sun CC Wang NF Chien (1973) ArticleTitleThe induction of the pollen plants of Triticale and Capsicum annuum from anther culture Sci. Sinica 16 147–151

  33. YB Wang H Hu JW Snape (1996) ArticleTitleThe genetic and molecular characterization of pollen-derived plant lines from octoploid triticale × wheat hybrids Theor. Appl. Genet. 92 811–816 Occurrence Handle10.1007/s001220050197

  34. MY Zheng W Liu Y Weng E Polle CF Konzak (2001) ArticleTitleCulture of freshly isolated wheat (Triticum aestivum L.) microspores treated with inducer chemicals Plant Cell Rep. 20 685–690 Occurrence Handle10.1007/s00299-001-0393-0

  35. MY Zheng Y Weng W Liu CF Konzak (2002) ArticleTitleThe effect of ovary-conditioned medium on microspore embryogenesis in common wheat (Triticum aestivum L.) Plant Cell Rep 20 802–807 Occurrence Handle10.1007/s00299-001-0411-2

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Correspondence to F. Eudes.

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Eudes, F., Amundsen, E. Isolated microspore culture of Canadian 6× triticale cultivars. Plant Cell Tiss Organ Cult 82, 233–241 (2005).

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  • androgenesis
  • cereal
  • doubled haploid