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Plant regeneration through somatic embryogenesis from callus cultures of Dioscorea zingiberensis

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A new method was established for somatic embryogenesis and plant regeneration from callus cultures of Dioscorea zingiberensis C.H. Wright. Primary callus was induced by culturing stems, leaves and petioles on Murashige and Skoog (MS) medium supplemented with 0.5–2.0 mg l−1 N6-benzyladenine (BA) and 0–2.0 mg l−1 α-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) for 1 month. The highest frequency (87%) of callus formation was achieved from stem explants treated with 0.5 mg l−1 BA and 2.0 mg l−1 2,4-D. Somatic embryos were obtained by subculturing embryogenic calli derived from stem explants on MS medium supplemented with 2.0–4.0 mg l−1 BA and 0–0.4 mg l−1 NAA or 2,4-D for 3 weeks. The optimum combination of 4.0 mg l−1 BA and 0.2 mg l−1 NAA promoted embryo formation on one-third of the calli. After a further month of subculture on the same medium, mature embryos were transferred to MS medium supplemented with 0–4.0 mg l−1 BA, NAA or indole-3-butyric acid (IBA) for further development of plantlets and tuber formation. Plant growth regulators had a negative effect on the development of mature embryos.

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2,4-dichlorophenoxyacetic acid




indole-3-butyric acid


α-naphthaleneacetic acid


  1. S Alizadeh SH Mantell AM Viana (1998) ArticleTitleIn vitro shoot culture and microtuber induction in the steroid yam Dioscorea composita Hemsl Plant Cell Tiss. Org. Cult. 53 107–112

  2. Jl John Wh Courtney DR Decoteau (1993) ArticleTitleThe influence of plant-growth regulators and light on microtuber induction and formation in Dioscorea alata L. cultures. Plant Cell Tiss. Org. Cult. 34 245–252

  3. H Kohmura H Araki M Imoto (1995) ArticleTitleMicropropagation of ‘yamatoimo’ chinese yam (Dioscorea opposita) from immature leaves Plant Cell Tiss. Org. Cult. 40 271–276

  4. EO Osifo (1988) ArticleTitleSomatic embryogenesis in Dioscorea J. Plant Physiol. 133 378–380

  5. M Tor CT Twyford I Funes J Boccon-Gibod CC Ainsworth SH Mantell (1998) ArticleTitleIsolation and culture of protoplasts from immature leaves and embryogenic cell suspensions of Dioscorea yams: tools for transient gene expression studies Plant Cell Tiss. Org. Cult. 53 113–125

  6. CT Twyford SH Mantell (1996) ArticleTitleProduction of somatic embryos and plantlets from root cells of the Greater Yam Plant Cell Tiss. Org. Cult. 46 17–26

  7. AM Viana SH Mantell (1989) ArticleTitleCallus induction and plant regeneration from excised zygotic embryos of the seed-propagated yams Dioscorea Composita Hemsl. And Dioscorea cayenensis Lam. Plant Cell Tiss. Org. Cult. 16 113–122

  8. YC Yan HH Lin QL Dai QQ Huang (2002) ArticleTitleStudies on tissue culture and rapid propagation of Dioscorea zingiberensis J. Sichuan University 39 136–140

  9. C Yongqin F Jinyu Y Fei L Zhongxun F Yunsheng (2003) ArticleTitleRapid clonal propagation of Dioscorea zingiberensis Plant Cell Tiss. Org. Cult. 73 75–80

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Correspondence to Lin Hong-Hui.

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Shu, Y., Ying-Cai, Y. & Hong-Hui, L. Plant regeneration through somatic embryogenesis from callus cultures of Dioscorea zingiberensis. Plant Cell Tiss Organ Cult 80, 157–161 (2005). https://doi.org/10.1007/s11240-004-9543-8

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  • callus formation
  • somatic embryos
  • stem explants
  • yams