A new method was established for somatic embryogenesis and plant regeneration from callus cultures of Dioscorea zingiberensis C.H. Wright. Primary callus was induced by culturing stems, leaves and petioles on Murashige and Skoog (MS) medium supplemented with 0.5–2.0 mg l−1 N6-benzyladenine (BA) and 0–2.0 mg l−1 α-naphthaleneacetic acid (NAA) or 2,4-dichlorophenoxyacetic acid (2,4-D) for 1 month. The highest frequency (87%) of callus formation was achieved from stem explants treated with 0.5 mg l−1 BA and 2.0 mg l−1 2,4-D. Somatic embryos were obtained by subculturing embryogenic calli derived from stem explants on MS medium supplemented with 2.0–4.0 mg l−1 BA and 0–0.4 mg l−1 NAA or 2,4-D for 3 weeks. The optimum combination of 4.0 mg l−1 BA and 0.2 mg l−1 NAA promoted embryo formation on one-third of the calli. After a further month of subculture on the same medium, mature embryos were transferred to MS medium supplemented with 0–4.0 mg l−1 BA, NAA or indole-3-butyric acid (IBA) for further development of plantlets and tuber formation. Plant growth regulators had a negative effect on the development of mature embryos.
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Shu, Y., Ying-Cai, Y. & Hong-Hui, L. Plant regeneration through somatic embryogenesis from callus cultures of Dioscorea zingiberensis. Plant Cell Tiss Organ Cult 80, 157–161 (2005). https://doi.org/10.1007/s11240-004-9543-8
- callus formation
- somatic embryos
- stem explants