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Plant Cell, Tissue and Organ Culture

, Volume 80, Issue 2, pp 221–228 | Cite as

A protocol for in vitro germination and sustainable growth of two tropical mistletoes

  • Sylvia L. P. Ang
  • Jean W. H. YongEmail author
Research note

Abstract

We report a protocol for in vitro germination and sustainable growth of two tropical mistletoes, Dendrophthoe pentandra and Macrosolen cochinchinensis . Normal mistletoe shoots with haustoria have been produced using Murashige and Skoog (MS) media supplemented with coconut water (15–20% v/v) in ventilated culture vessels. Germination of mistletoes took place on hormone-free basal MS media, and the time needed for germination was much shorter for Macrosolen than for Dendrophthoe . After germination, further development of the mistletoes required a hormonal input from the medium. We found that 20 μM 6-benzylaminopurine (BA) did allow normal development of only Dendrophthoe , while coconut water (15–20% v/v) gave complete development of both species in ventilated culture vessels. Multiplication of these mistletoes is also possible via the callus stage using half-strength MS media supplemented with BA at various concentrations in ventilated culture vessels. This is probably the first report of a successful culture for the mistletoe genus of Macrosolen . Compared to Macrosolen , the nutritional and hormonal requirements for Dendrophthoe are less demanding, making the latter a useful model for further mistletoe research.

Keywords

coconut water cytokinin Dendrophthoe Marcosolen ventilated culture vessels 

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Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Natural Sciences Academic GroupNanyang Technological UniversitySingaporeSingapore

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