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Plant Cell, Tissue and Organ Culture

, Volume 81, Issue 3, pp 307–311 | Cite as

Control of growth and differentiation of bioreactor cultures of Physcomitrella by environmental parameters

  • Annette Hohe
  • Ralf Reski
Article

Abstract

The effect of physical and chemical environmental parameters on growth and differentiation of suspension cultures of the moss Physcomitrella patens in bioreactors was investigated. By supplementation of the aeration gas with 2% (v/v) CO2 as well as by continuous illumination, growth of this photoautotrophic growing batch culture was markedly enhanced, resulting in a doubling time of 1.2 d. The growth rate of semicontinuously growing bioreactor cultures was not affected by controlling the pH of the culture medium with set points at 4.5 or 7.0. However, growth of the culture at pH 7.0 resulted in increased caulonema development, thus showing a distinct effect on moss differentiation. The impact on research and plant biotechnological applications of the potential to control moss growth and differentiation by environmental parameters is discussed.

Keywords

Key words CO2 light moss pH photoautotrophic culture suspension culture 

Abbreviations

vvm

volume of gas per volume of liquid per minute

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Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Department of Plant PropagationInstitute of Vegetable and Ornamental CropsKuehnhausenGermany
  2. 2.Plant BiotechnologyFreiburg UniversityFreiburgGermany

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