Plant Cell, Tissue and Organ Culture

, Volume 81, Issue 3, pp 287–300 | Cite as

Application of bioreactor systems for large scale production of horticultural and medicinal plants

Abstract

Automation of micropropagation via organogenesis or somatic embryogenesis in a bioreactor has been advanced as a possible way of reducing costs. Micropropagation by conventional techniques is typically a labour-intensive means of clonal propagation. The paper describes lower cost and less labour-intensive clonal propagation through the use of modified air-lift, bubble column, bioreactors (a balloon-type bubble bioreactor), together with temporary immersion systems for the propagation of shoots, bud-clusters and somatic embryos. Propagation of Anoectochilus, apple, Chrysanthemum, garlic, ginseng, grape, Lilium, Phalaenopsis and potato is described. In this chapter, features of bioreactors and bioreactor process design specifically for automated mass propagation of several plant crops are described, and recent research aimed at maximizing automation of the bioreactor production process is highlighted.

Keywords

Anoectochilus apple automated masspropagation Chrysanthemum dissolved oxygen garlic ginseng grape Lilium micropropagation mixing nutrients pH Phalaenopsis potato secondary metabolites siberian ginseng somatic embryogenesis 

Abbreviations

BTBB

balloon type bubble bioreactor

DO

dissolved oxygen

IEDC

induced embryogenic determined cells

PLB

protocorm-like body

PPF

photosynthetic photon flux

STR

stirred tank reactor

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Copyright information

© Springer 2005

Authors and Affiliations

  1. 1.Research Center for the Development of Advanced Horticultural TechnologyChungbuk National UniversityCheongjuSouth Korea

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