Plant Cell, Tissue and Organ Culture

, Volume 81, Issue 2, pp 193–202 | Cite as

Cryopreservation of Robinia pseudoacacia

  • H Verleysen
  • P Fernandes
  • I. Sánchez Pinto
  • E. Van Bockstaele
  • P Debergh


Cryopreservation of Robinia pseudoacacia explants by vitrification achieved 78% survival following the stepwise preculture of shoot tips in (0.3 + 0.5 + 0.7 M) sucrose with a 80 min incubation in PVS2; compared to 87% survival after desiccation of explants to 30% water content, following 3 days alginate bead (with glycerol and sucrose treatments) preculture in 0.7 M sucrose.


alginate black locust encapsulation-dehydration glycerol PVS2 Robinia pseudoacacia sucrose vitrification 





liquid nitrogen


Murashige and Skoog medium (Murashige and Skoog, 1962)


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Copyright information

© Springer 2005

Authors and Affiliations

  • H Verleysen
    • 1
    • 2
  • P Fernandes
    • 1
  • I. Sánchez Pinto
    • 1
  • E. Van Bockstaele
    • 1
    • 2
  • P Debergh
    • 1
  1. 1.Department of Plant Production, Faculty of Bioscience EngineeringUniversity GentGentBelgium
  2. 2.Department of Plant Genetics and BreedingCLO GentMelleBelgium

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