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Plant Cell, Tissue and Organ Culture

, Volume 81, Issue 2, pp 193–202 | Cite as

Cryopreservation of Robinia pseudoacacia

  • H Verleysen
  • P Fernandes
  • I. Sánchez Pinto
  • E. Van Bockstaele
  • P Debergh
Article

Abstract

Cryopreservation of Robinia pseudoacacia explants by vitrification achieved 78% survival following the stepwise preculture of shoot tips in (0.3 + 0.5 + 0.7 M) sucrose with a 80 min incubation in PVS2; compared to 87% survival after desiccation of explants to 30% water content, following 3 days alginate bead (with glycerol and sucrose treatments) preculture in 0.7 M sucrose.

Keywords

alginate black locust encapsulation-dehydration glycerol PVS2 Robinia pseudoacacia sucrose vitrification 

Abbreviations

DMSO

dimethylsulfoxide

LN

liquid nitrogen

MS

Murashige and Skoog medium (Murashige and Skoog, 1962)

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References

  1. Bouman, H, Klerk, GJ 1990Cryopreservation of lily meristemsActa Hortic.266331337Google Scholar
  2. Burke, MJ 1987The glassy state and survival of anhydrous biological systemsLeopold, AC eds. Membranes, Metabolism and Dry OrganismsCornell University PressIthaca, NY35833564Google Scholar
  3. Proft, MP, Maene, LJ, Debergh, PC 1985Carbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitroPhysiol. Plant.65375379Google Scholar
  4. Dereuddre, J, Blandin, S, Hassen, N 1991Resistence of alginate-coated shoot tips of pear tree (Pyrus communis L. cv Beurré Hardy) in vitro plantlets to dehydration and subsequent freezing in liquid nitrogen: effects of previous cold hardeningComptes rendues de l’académie de sciences Paris310317323Google Scholar
  5. Engelmann, F 2000Importance of cryopreservation for the conservation of plant genetic resourcesEngelmann, FTakagi, H eds. Cryopreservation of Tropical GermplasmCurrent Research Progress and ApplicationJIRCAS, Rome820Google Scholar
  6. Fahy, GM, McFarlane, DR, Angell, CA, Meryman, HT 1984Vitrification as an approach to cryopreservationCryobiology3407426Google Scholar
  7. Gonzalez-Arnao, MT, Engelmann, F, Villavicencio, CU, Morenza, M, Rios, A 2000Cryopreservation of citrus apices using the encapsulation-dehydration techniqueEngelmann, FTakagi, H eds. Cryopreservation of Tropical GermplasmCurrent Research Progress and ApplicationJIRCAS, Rome217221Google Scholar
  8. Grapin, A, Dumet, D, Holota, H, Dorion, N 2003Cryoreservation of Pelargonium shoot tips by encapsulation-dehydration: effects of sucrose concentration, dehydration duration and genotypeActa Hortic.623225230Google Scholar
  9. Grout, BWW 1995Cryopreservation of excised meristems, shoot tips and somatic embryosGrout, BWW eds. Genetic Preservation of Plant Cells in vitroSpringer-VerlagBerlin4762Google Scholar
  10. Hillier J & Coombes A (1998). The Hillier Manual of Trees & Shrubs. Newton Abbot, David & Charles.Google Scholar
  11. Hirata, K, Monthana, P, Sakai, A, Miyamoto, K 1996Cryopreservation of encapsulated shoot primordials induced in horseradish (Armoracia rusticana) hairy root culturesPlant Cell Rep.16469473Google Scholar
  12. Lambardi, M, Fabbri, A, Caccavale, A 2000Cryopreservation of white poplar (Populus alba L.) by vitrification of in vitro grown shoot tipsPlant Cell Rep.19213218Google Scholar
  13. Martinez, D, Tamés, RS, Revilla, MA 1999Cryopreservation of in vitro-grown shoot tips of hop (Humulus lupulus L.) using encapsulation-dehydrationPlant Cell Rep.195963Google Scholar
  14. Matsumoto, T, Mochida, K, Itamura, H, Sakai, A 2001Cryopreservation of persimmon (Diospyros kaki Thunb.) by vitrification of dormant shoot tipsPlant Cell Rep.20398402Google Scholar
  15. Murashige, T 1977Plant cell and organ cultures as horticultural practicesActa Hortic.781730Google Scholar
  16. Murashige, T, Skoog, F 1962A revised medium for rapid growth and bioassays with tobacco tissue culturesPlant Physiol.15473497Google Scholar
  17. Niino, T, Tahiro, K, Suzuki, M, Ochuchi, S, Magoshi, J, Akihama, T 1997Cryopreservation of in vitro grown shoot tips of cherry and sweet cherry by one-step vitrificationSci. Hortic.70155163Google Scholar
  18. Niino, T, Sakai, A 1992Cryopreservation of alginate-coated in vitro-grown shoot tips of apple, pear and mulberryPlant Sci.87199206Google Scholar
  19. Pâques, M, Monod, V, Poissonnier, M, Dereuddre, J 2002Cryopreservation of Eucalyptus sp. shoot tips by the encapsulation-dehydration procedureTowill, LEBajaj, YPS eds. Biotechnology in Agriculture and Forestry 50Springer-VerlagBerlin234245Google Scholar
  20. Pâques, M, Poisonnier, M, Dumas, E, Monod, V 1997Cryopreservation of dormant and non-dormant broad-leaved treesActa Hortic.447491497Google Scholar
  21. Paul, H, Gaigny, G, Sangwan-Norreel, BS 2000Cryopreservation of apple (Malus × domestica Borkh.) shoot tips following encapsulation-dehydration or encapsulation-vitrificationPlant Cell Rep.19768774Google Scholar
  22. Reinhoud, PJ, Schrijnemakers, WM, Iren, F, Kijne, JW 1995Vitrification and a heat shock treatment improve cryopreservation of tobacco cell suspension compared to two-step freezingPlant Cell Tiss. Org.42261267Google Scholar
  23. Sakai, A, Kobayashi, S, Oiyama, I 1990Cryopreservation of nucellar cells of navel orange (Citrus sinensis Obs var. brasiliensis Tanaka) by vitrificationPlant Cell Rep.93033Google Scholar
  24. Seufferheld, MJ, Stushnoff, C, Forsline, P, Gonzalez, GHT 1999Cryopreservation of cold-tender apple germplasmJ. Am. Soc. Hortic. Sci.124612618Google Scholar
  25. Shatnawi, MA, Johnson, KA 2004Cryopreservation by encapsulation-dehydration of ‘Christmas bush’ (Ceratopetalum gummiferum) shoot tipsIn Vitro Cell. Dev.40239244Google Scholar
  26. Takagi, H 2000Recent developments in cryopreservation of shoot apices of tropical speciesEngelman, FTakagi, H eds. Cryopreservation of Tropical GermplasmCurrent Research Progress and ApplicationJIRCAS, Rome178193Google Scholar
  27. Takagi, H, Thinh, NT, Islam, OM, Senboku, T, Sakai, A 1997Cryopreservation of in vitro grown shoot tips of taro (Colocasia esculenta Scott.) by vitrificationPlant Cell Rep.16594599Google Scholar
  28. Towill, LE 1995Cryopreservation by vitrificationGrout, BWW eds. Genetic Preservation of Plant Cells in vitroSpringer-VerlagBerlin99111Google Scholar
  29. Tsuruta, T, Ishimoto, Y, Masuoka, T 1998Effects of glycerol on intracellular ice formation and dehydration of onion epidermisAnn. NY Acad. Sci.858217226Google Scholar
  30. Vandenbussche, B, Wayens, G, Proft, M 2000Cryopreservation of in vitro sugar beet (Beta vulgaris L.) shoot tips by a vitrification techniquePlant Cell Rep.1910641068Google Scholar
  31. Verleysen, H, Samyn, G, Bockstaele, E, Debergh, P 2004Evaluation of analytical techniques to predict viability after cryopreservationPlant Cell Tiss. Org. Cult.771121Google Scholar
  32. Wolfe, J, Bryant, G 2001Cellular cryobiology: thermodynamic and mechanical effectsInt. J. Refrig.24438450Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • H Verleysen
    • 1
    • 2
  • P Fernandes
    • 1
  • I. Sánchez Pinto
    • 1
  • E. Van Bockstaele
    • 1
    • 2
  • P Debergh
    • 1
  1. 1.Department of Plant Production, Faculty of Bioscience EngineeringUniversity GentGentBelgium
  2. 2.Department of Plant Genetics and BreedingCLO GentMelleBelgium

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