Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

In vitro propagation of the genus Clivia

  • 238 Accesses

  • 5 Citations

Abstract

We have developed a protocol for the in vitro propagation of the genus Clivia. Shoots were regenerated when fragments of the peduncle-pedicel junction (PP junction) from young inflorescences were used as explants. The optimal media for PP junction were Murashige and Skoog (MS)-based medium containing 10 μM of 6-benzyladenine (BA) and 10 μM of 2,4-dichlorophenoxyacetic acid (2,4-D) or MS supplemented with 5 μM BA, 10 μM α-naphthaleneacetic acid (NAA), 250 mg l-1 glutamine and 500 mg l−1 casein hydrolysate and their usage depended on the breeding lines. Multiplication from initiations and in vitro seedlings was the best when the explants were cut longitudinally through the meristem and placed on MS plus 44 μM BA. Plantlets were transferred on to hormone -free MS medium with charcoal for rooting.

This is a preview of subscription content, log in to check access.

Abbreviations

BA:

6-benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

α-naphthaleneacetic acid

MS:

Murashige and Skoog (1962) medium

PP junction:

peduncle-pedicel junction

References

  1. AC Chapman (1999) In vitro propagation of Clivia miniata Bsc Honours Thesis University of Nottingham United Kingdom

  2. MN De Bruyn DJ Ferreira MM Slabbert J Pretorius (1992) ArticleTitleIn vitro propagation of Amaryllis belladonna Plant Cell Tiss. Org. Cult. 31 179–184

  3. C Giddy (1982) ArticleTitleThe noble Clivia Natal Gardener 10 11–12

  4. KW Kim AA De Hertogh (1996) Tissue culture of ornamental flowering bulbs (Geophytes) J Janick (Eds) Horticultural Reviews, Vol 18 John Wiley and Sons Inc. London 87–169

  5. L Min S Jingshen (1984) ArticleTitleTissue culture of Kafirlily (Clivia nobilis) Acta Hort. Sin. 11 47–49

  6. T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassay with tobacco tissue culture Physiol. Plant. 15 273–297

  7. JP Rourke (2002) ArticleTitleClivia mirabilis (Amaryllidaceae: Haemantheae) a new species from Northern Cape, South Africa Bothalia 32 1–7

  8. M Ziv H Lilien-Kipnis (2000) ArticleTitleBud regeneration from inflorescence explants for rapid propagation of geophytes in vitro Plant Cell Rep. 19 845–850

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ran, Y., Simpson, S. In vitro propagation of the genus Clivia. Plant Cell Tiss Organ Cult 81, 239–242 (2005). https://doi.org/10.1007/s11240-004-3229-0

Download citation

Keywords

  • Clivia
  • micropropagation
  • peduncle–pedicel junction