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Production of interspecific somatic hybrids between tuber mustard (Brassica juncea) and red cabbage (Brassica oleracea)

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Abstract

In the present investigation, the interspecific somatic hybridization between tuber mustard and red cabbage was established in order to introduce valuable genes from red cabbage (Brassica oleracea) into Brassica juncea. Prior to fusion treatment, protoplasts of red cabbage were inactivated with 2 mM iodoacetamide to inhibit cell division. Micro-calluses were obtained at a frequency of 10.3% after approximately 5 weeks culture following protoplast fusion. Some of the fusion-derived calluses possessed red pigmented cells after being transferred to proliferation medium, and they were presumably considered to be somatic hybrid cell lines. Plantlets were regenerated from 12 cell lines, of which nine plantlets exhibited characteristics intermediate of both parents in terms of plant morphology. With the exception of common protein bands featured by two parents, there were unique banding patterns produced in the hybrids by using SDS-PAGE analysis. By chromosome countings, it was showed that they ranged approximately from 2n=30 to 42 in chromosome numbers. Their hybridity were further confirmed by RAPD analysis revealing that genes of both parents were partially incorporated into the hybrids. Positively, all these hybrids were capable of seed-setting. The pod-setting was 4.2 in somatic hybrid H7 when backcrossed with tuber mustard.

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Abbreviations

BA:

benzyladenine

ß-ME:

ß-Mercaptoethanol

CPW:

Cell and Protoplast Washing Solution

2,4-D:

2,4-dichlorophenoxyacetic acid

MES:

2-(N-morpholino)-ethane-sulfonic acid

MS:

Murashige and Skoog (1962) medium

NAA:

α-naphthaleneacetic acid

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Correspondence to Li-Ping Chen.

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Chen, L., Zhang, M., Li, C. et al. Production of interspecific somatic hybrids between tuber mustard (Brassica juncea) and red cabbage (Brassica oleracea). Plant Cell Tiss Organ Cult 80, 305–311 (2005). https://doi.org/10.1007/s11240-004-2447-9

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Keywords

  • Brassica juncea
  • Brassica oleracea
  • protein analysis
  • protoplast fusion
  • RAPD