Plant Cell, Tissue and Organ Culture

, Volume 80, Issue 3, pp 347–351 | Cite as

Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium

  • S. Prathanturarug
  • N. Soonthornchareonnon
  • W. Chuakul
  • Y. Phaidee
  • P. Saralamp
Research Note


Multiple shoots of Curcuma longa were induced by culture of bud explants for 1 week in Murashige and Skoog (MS) liquid medium supplemented with 72.64 μM thidiazuron (TDZ) prior to culture on MS gelled medium without growth regulator for 8 weeks. The regeneration rate was up to 11.4 ± 1.7 shoots/explant. Rooting was spontaneous and the regenerated plants were successfully transferred to soil. This protocol can be an alternative for rapid micropropagation of C. longa used for phytomedicine raw material production.


liquid medium thidiazuron Turmeric 




MS medium

Murashige and Skoog medium


plant growth regulator




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Copyright information

© Springer 2005

Authors and Affiliations

  • S. Prathanturarug
    • 1
  • N. Soonthornchareonnon
    • 2
  • W. Chuakul
    • 1
  • Y. Phaidee
    • 1
  • P. Saralamp
    • 1
  1. 1.Department of Pharmaceutical Botany, Faculty of PharmacyMahidol UniversityBangkokThailand
  2. 2.Department of Pharmacognosy, Faculty of PharmacyMahidol UniversityBangkokThailand

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