Advertisement

Plant Cell, Tissue and Organ Culture

, Volume 80, Issue 3, pp 347–351 | Cite as

Rapid micropropagation of Curcuma longa using bud explants pre-cultured in thidiazuron-supplemented liquid medium

  • S. Prathanturarug
  • N. Soonthornchareonnon
  • W. Chuakul
  • Y. Phaidee
  • P. Saralamp
Research Note

Abstract

Multiple shoots of Curcuma longa were induced by culture of bud explants for 1 week in Murashige and Skoog (MS) liquid medium supplemented with 72.64 μM thidiazuron (TDZ) prior to culture on MS gelled medium without growth regulator for 8 weeks. The regeneration rate was up to 11.4 ± 1.7 shoots/explant. Rooting was spontaneous and the regenerated plants were successfully transferred to soil. This protocol can be an alternative for rapid micropropagation of C. longa used for phytomedicine raw material production.

Keywords

liquid medium thidiazuron Turmeric 

Abbreviations

BA

6-benzylaminopurine

MS medium

Murashige and Skoog medium

PGR

plant growth regulator

TDZ

thidiazuron

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Balachandran, SM, Bhat, SR, Chandel, KPS 1990In vitro clonal multiplication of turmeric (Curcuma spp.) and ginger (Zingiber officinale Rosc.)Plant Cell Rep.8521524Google Scholar
  2. Etienne, H, Berthouly, M 2002Temporary immersion systems in plant micropropagationPlant Cell Tiss. Org. Cult.69215231Google Scholar
  3. George, EF 1993Plant Propagation by Tissue Culture Part 1.The TechnologyExegetics, EdingtonGoogle Scholar
  4. Huetteman, CA, Preece, JE 1993Thidiazuron: a potent cytokinin for woody plant tissue culturePlant Cell Tiss. Org. Cult.33105119Google Scholar
  5. Kositchaiwat, C, Kositchaiwat, S, Havanondha, J 1993Curcuma longa Linn. in the treatment of gastric ulcer comparison to liquid antacid: a controlled clinical trialJ. Med. Assoc. Thai.76601605Google Scholar
  6. Murashige, T, Skoog, F 1962A revised medium for rapid growth and bioassays with tobacco tissue culturesPhysiol. Plant.15473497Google Scholar
  7. Prathanturarug, S, Soonthornchareonnon, N, Chuakul, W, Phaidee, Y, Saralamp, P 2003High-frequency shoot multiplication in Curcuma longa L. using thidiazuronPlant Cell Rep.2110541059Google Scholar
  8. Prucksunand, C, Indrasukhsri, B, Leethochawalit, M, Hungspreugs, K 2001Phase II clinical trial on effect of the long turmeric (Curcuma longa Linn) on healing of peptic ulcerSoutheast Asian J. Trop. Med. Public Health32208215Google Scholar
  9. Salvi, N, George, L, Eapen, S 2000Direct regeneration of shoots from immature inflorescence cultures of turmericPlant Cell Tiss. Org. Cult.62235238Google Scholar
  10. Salvi, N, George, L, Eapen, S 2001Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plantsPlant Cell Tiss. Org. Cult.66113119Google Scholar
  11. Salvi, N, George, L, Eapen, S 2002Micropropagation and field evaluation of micropropagated plants of turmericPlant Cell Tiss. Org. Cult.68143151Google Scholar
  12. Shirgurkar, M, John, C, Nadgauda, R 2001Factor affecting in vitro microrhizome production in turmericPlant Cell Tiss. Org. Cult.64511Google Scholar
  13. Sunitibala, H, Damayanti, M, Sharma, G 2001In vitro propagation and rhizome formation in Curcuma longa LinnCytobios1057182Google Scholar
  14. Thamlikitkul, V, Bunyapraphatsara, N, Dechatiwongse, T, Theerapong, S, Chantrakul, C, Thanaveerasuwan, T, Nimitnon, S, Boonroj, P, Punkrut, W, Gingsungneon, V, Wongkonkatape, S, Ekpalakorn, W, Boontaeng, N, Jesadaporn, U, Taechaiya, S, Petcharoen, S, Riewpaiboon, A, Adthasit, R, Timsard, S, Tenambergen, ED 1989Randomized double blind study of Curcuma domestica Val. for dyspepsiaJ. Med. Assoc. Thai.72613620Google Scholar
  15. World Health Organization1999WHO Monographs on Selected Medicinal PlantsWHOGenevaVol. 1Google Scholar
  16. Yasuda, K, Tsuda, T, Shimizu, H, Sugaya, A 1988Multiplication of Curcuma species by tissue culturePlant. Med.547578Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • S. Prathanturarug
    • 1
  • N. Soonthornchareonnon
    • 2
  • W. Chuakul
    • 1
  • Y. Phaidee
    • 1
  • P. Saralamp
    • 1
  1. 1.Department of Pharmaceutical Botany, Faculty of PharmacyMahidol UniversityBangkokThailand
  2. 2.Department of Pharmacognosy, Faculty of PharmacyMahidol UniversityBangkokThailand

Personalised recommendations