Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

The effect of aminoglycoside antibiotics on the adventitious regeneration from apricot leaves and selection of nptII-transformed leaf tissues


The effect of different concentrations of the aminoglycoside antibiotics, geneticin, paromomycin and streptomycin on adventitious regeneration from leaf explants of apricot was tested to design an alternative procedure for selecting transgenic shoots. Streptomycin and paromomycin reduced shoot regeneration percentage with increasing concentration of antibiotics. Almost a complete inhibition of regeneration was reached when 20μM paromomycin was used, although up to 40 μM streptomycin was necessary to completely inhibit regeneration. Geneticin had a very toxic effect on apricot leaves and regeneration was inhibited at almost all concentrations tested. Addition of kanamycin hastened the development of adventitious buds although silver thiosulfate and not kanamycin was responsible for the observed increase in the consistency of the results from independent experiments. Kanamycin and paromomycin at the concentrations tested improved selection of transformed cells and resulted in a larger number of gfp-expressing regions. Paromomycin at 40 and 25.7 μM kanamycin improved proliferation of transformed tissues as compared with the other antibiotics used and non-selected controls.

This is a preview of subscription content, log in to check access.





coefficient of variation


Driver and Kuniyuki (1984)


green fluorescent protein


naphthaleneacetic acid


Quoirin and Lepoivre (1977)


silver thiosulphate




  1. J Alt-Mörbe H Kühlmann J Schröder (1989) ArticleTitleDifferences in induction of Ti plamid virulence genes virG and virD and continued control of virD expression by four external factors Mol. Plant–Microbe Interact. 2 301–308

  2. L Burgos N Alburquerque (2003) ArticleTitleLow kanamycin concentration and ethylene inhibitors improve adventitious regeneration from apricot leaves Plant Cell Rep. 21 1167–1174

  3. E Chevreau F Mourgues M Neveu M Chevalier (1997) ArticleTitleEffect of gelling agents and antibiotics on adventitious bud regeneration from in vitroleaves of pear In Vitro Cell. Dev. Biol. -Plant 33 173–179

  4. SM Colby CP Meredith (1990) ArticleTitleKanamycin sensitivity of cultured tissues of Vitis Plant Cell Rep. 9 237–240

  5. AM Dandekar GH McGranahan PV Vail SL Uratsu C Leslie JS Tebbets (1998) ArticleTitleHigh levels of expression of full length cryIA(c) gene from Bacillus thuringiensis in transgenic somatic walnut embryos Plant Sci. 131 181–193

  6. A Domínguez J Guerri M Cambra L Navarro P Moreno L Peña (2000) ArticleTitleEfficient production of transgenic citrus plants expressing the coat protein gene of citrus tristeza virus Plant Cell Rep. 19 427–433

  7. JA Driver AH Kuniyuki (1984) ArticleTitleIn vitro propagation of Paradox walnut rootstock HortScience 19 507–509

  8. S Eapen L George (1990) ArticleTitleInfluence of phytohormones, carbohydrates, aminoacids, growth supplements and antibiotics on somatic embryogenesis and plant differentiation in finger millet Plant Cell Tiss. Org. Cult. 22 87–93

  9. V Escalettes F Dosba (1993) ArticleTitleIn vitro adventitious shoot regeneration from leaves of Prunus spp Plant Sci. 90 201–209

  10. FA Hammerschlag RH Zimmerman UL Yadava S Hunsucker P Gercheva (1997) ArticleTitleEffect of antibiotics and exposure to an acidified medium on the elimination of Agrobacterium tumefaciens from apple leaf explants and on shoot regeneration J. Am. Soc. Hortic. Sci. 122 758–763

  11. JM Humara RJ Ordás (1999) ArticleTitleThe toxicity of antibiotics and herbicides on in vitro adventitious shoot formation on Pinus pinea L.cotyledons In Vitro Cell. Dev. Biol. -Plant 35 339–343

  12. M Mencuccini M Micheli A Angiolillo L Baldoni G Frugis D Mariotti (1999) ArticleTitleAgrobacterium mediated DNA transfer in olive callus (Olea europaea L.) Adv. Hort. Sci. 13 25–28

  13. CM Miguel MM Oliveira (1999) ArticleTitleTransgenic almond (Prunus dulcis Mill.)plants obtained by Agrobacteriummediated transformation of leaf explants Plant Cell Rep. 18 387–393

  14. JL Norelli HS Aldwinckle (1993) ArticleTitleThe role of aminoglycoside antibiotics in the regeneration and selection of Neomycin Phosphotransferase-transgenic apple tissue J. Am. Soc. Hortic. Sci. 118 311–316

  15. LD Owens (1979) ArticleTitleKanamycin promotes morphogenesis of plant tissues Plant Sci. Lett. 16 225–230

  16. Petri C, Alburquerque N, García-Castillo S, Egea J & Burgos L (in press) Factors affecting gene transfer efficiency to apricot leaves during early Agrobacterium-mediated transformation steps. J. Hortic. Sci. Biotech.

  17. O Pérez-Tornero L Burgos (2000) ArticleTitleDifferent media requirements for micropropagation of apricot cultivars Plant Cell Tiss. Org. Cult. 63 133–141

  18. O Pérez-Tornero J Egea A Vanoostende L Burgos (2000) ArticleTitleAssessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot Plant Sci. 158 61–70

  19. M Quoirin P Lepoivre (1977) ArticleTitleEtude de milieux adaptes aux cultures in vitro de Prunus Acta Hort. 78 437–442

  20. G Rajashekar DE Palmquist CA Ledbetter (1995) ArticleTitleIn vitro screening procedure for osmotic tolerance in Prunus Plant Cell Tiss. Org. Cult. 41 159–164

Download references

Author information

Correspondence to Lorenzo Burgos.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Petri, C., Alburquerque, N. & Burgos, L. The effect of aminoglycoside antibiotics on the adventitious regeneration from apricot leaves and selection of nptII-transformed leaf tissues. Plant Cell Tiss Organ Cult 80, 271–276 (2005).

Download citation


  • adventitious regeneration
  • Agrobacterium-mediated transformation
  • Prunus armeniaca L
  • selective marker