Exosome mediated differentiation of megakaryocytes: a study on TLR mediated effects
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Megakaryocytes are large polyploid bone marrow cells whose function is to produce circulatory platelets. Megakaryocytes are also known to release extracellular vesicles (EVs) of varying sizes. Toll like receptors (TLRs), present on the sentinel cells are essential components of the innate immune response, these receptors are also expressed by platelets and megakaryocytes. Our data provide the evidence that TLR-2 induced MKEVs are able to recapitulate TLR-2 signalling in megakaryocytic cell line (Dami cells) and that likely induces megakaryocytic maturation by increasing the production of cytokines involved in MK maturation. TLR-2 induced MKEVs may be involved in replenishment of the immune effector platelets in circulation and its progenitor megakaryocyte in bone marrow for the physiological need of the platelets by inducing the maturation of megakaryocyte.
KeywordsMegakaryocyte Extracellular vesicles Exosomes TLR-2
This work was supported by Department of Biotechnology (DBT), Department of Science and Technology (DST, Grant No. EEQ/2018/000853), Indian Council of Medical Research (ICMR) and University Grants Commission (UGC-SAP-DRS, UPE-2) grants of Government of India. We appreciate the funding in form of Council of Scientific and Industrial Research (CSIR) and UGC Fellowships from Government of India.
N.K. designed and performed experiments, and prepared the manuscript. S.R. prepared the manuscript and R.G. designed and supervised the study.
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Conflicts of interest
The authors declare that they have no conflict of interest.