Exosome mediated differentiation of megakaryocytes: a study on TLR mediated effects

  • Narasaiah Kovuru
  • Sanjeev Raghuwanshi
  • Ravi Kumar GuttiEmail author
Letter to the Editor


Megakaryocytes are large polyploid bone marrow cells whose function is to produce circulatory platelets. Megakaryocytes are also known to release extracellular vesicles (EVs) of varying sizes. Toll like receptors (TLRs), present on the sentinel cells are essential components of the innate immune response, these receptors are also expressed by platelets and megakaryocytes. Our data provide the evidence that TLR-2 induced MKEVs are able to recapitulate TLR-2 signalling in megakaryocytic cell line (Dami cells) and that likely induces megakaryocytic maturation by increasing the production of cytokines involved in MK maturation. TLR-2 induced MKEVs may be involved in replenishment of the immune effector platelets in circulation and its progenitor megakaryocyte in bone marrow for the physiological need of the platelets by inducing the maturation of megakaryocyte.


Megakaryocyte Extracellular vesicles Exosomes TLR-2 



This work was supported by Department of Biotechnology (DBT), Department of Science and Technology (DST, Grant No. EEQ/2018/000853), Indian Council of Medical Research (ICMR) and University Grants Commission (UGC-SAP-DRS, UPE-2) grants of Government of India. We appreciate the funding in form of Council of Scientific and Industrial Research (CSIR) and UGC Fellowships from Government of India.

Author contributions

N.K. designed and performed experiments, and prepared the manuscript. S.R. prepared the manuscript and R.G. designed and supervised the study.

Compliance with ethical standards

Conflicts of interest

The authors declare that they have no conflict of interest.


  1. 1.
    Paschon V, Takada SH, Ikebara JM, Sousa E, Raeisossadati R, Ulrich H, Kihara AH (2016) Interplay between exosomes, microRNAs and toll-like receptors in brain disorders. Mol Neurobiol 53(3):2016–2028CrossRefGoogle Scholar
  2. 2.
    Tao SC, Guo SC, Zhang CQ (2017) Platelet-derived extracellular vesicles: an emerging therapeutic approach. Int J Biol Sci 13(7):828–834CrossRefGoogle Scholar
  3. 3.
    Butler JT, Abdelhamed S, Kurre P (2018) Extracellular vesicles in the hematopoietic microenvironment. Haematologica 103(3):382–394CrossRefGoogle Scholar
  4. 4.
    Jiang J, Kao CY, Papoutsakis ET (2017) How do megakaryocytic microparticles target and deliver cargo to alter the fate of hematopoietic stem cells? J Controll Release 10(247):1–18CrossRefGoogle Scholar
  5. 5.
    Srinivasan S, Su M, Ravishankar S et al (2017) TLR-exosomes exhibit distinct kinetics and effector function. Sci Rep 7:41623CrossRefGoogle Scholar
  6. 6.
    Jayachandran M, Brunn GJ, Karnicki K, Miller RS, Owen WG, Miller VM (2007) In vivo effects of lipopolysaccharide and TLR4 on platelet production and activity: implications for thrombotic risk. J Appl Physiol 102(1):429–433CrossRefGoogle Scholar
  7. 7.
    Undi RB, Sarvothaman S, Narasaiah K, Gutti U, Gutti RK (2016) Toll-like receptor 2 signalling: significance in megakaryocyte development through wnt signalling cross-talk and cytokine induction. Cytokine 83:245–249CrossRefGoogle Scholar
  8. 8.
    Flaumenhaft R, Dilks JR, Richardson J, Alden E, Patel-Hett SR, Battinelli E, Klement GL, Sola-Visner M, Italiano JE Jr (2009) Megakaryocyte-derived micro particles: direct visualization and distinction from platelet-derived microparticles. Blood 113(5):1112–1121CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Biochemistry, School of Life SciencesUniversity of HyderabadHyderabadIndia

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