Individual differences of plasma proteins and factors in fresh frozen plasma from Chinese regional blood donors
Currently, single fresh frozen plasma (FFP) for clinical use is derived from individual blood donors. The objective of this study is to investigate the differences in single FFP units to make related strategies for improving FFP transfusion efficacy. 120 units of single FFP were selected randomly from Chinese PLA Clinical Blood Transfusion Center in Beijing, China. Single FFP samples were thawed in 37 °C water bath and were assessed immediately for total protein (TP), albumin (Alb), fibrinogen (Fg), factor V (FV), factor VIII (FVIII), antithrombin-III (AT-III) and protein C (PC). Multiple comparisons were employed to analysis the differences. The quality levels of 120 single FFP units showed wider ranges and presented normal distribution. Fg, FV and PC showed larger fluctuation range but TP, Alb, FVIII and AT-III showed smaller. The mean level of TP was 56.07 g/L and FVIII was 0.62 IU/mL. There were no significant differences to genders in single FFP units. FFP from younger people with 18–30 years old showed a trend towards reduced activity of coagulation factors, especially FV and FVIII. TP and Alb levels in FFP with type O were significantly higher than that in non-O type, but FVIII, AT-III and PC levels were lower in type O than that in non-O type. In a word, the whole quality of single FFP units from Chinese regional donors was acceptable, except FVIII. There were great differences of plasma proteins and factors in single FFP units, age and ABO blood type were main influence factors.
KeywordsIndividual differences Fresh frozen plasma Plasma proteins and factors Blood donors Chinese
Fresh frozen plasma
We thank experimental supports of Fengyan Fan, Liping Sun, Dongqing Zhang from Department of Blood Transfusion, Chinese PLA General Hospital and Ping Di from Department of Clinical Laboratory, Chinese PLA General Hospital.
This study was supported by the CAMS Innovation Fund for Medical Sciences (CIFMS, No. 2017-I2M-3-021), the Sichuan Provincial Science and Technology Project (No. 2017JY0064), and the Military Major Special Project (AWS11J007-08).
Compliance with ethical standards
Conflict of interest
The authors have disclosed no conflicts of interest.
The Institutional Ethics Committee of CPGH approved this study.
Informed consent was obtained from all individual participants included in the study.
- 3.National Health and Family Planning Commission of the People’s Republic of China (2000) The Technical Criterion of Clinical Blood TransfusionGoogle Scholar
- 4.European Parliament. Commission directive 2004/33/EC. Official Journal of the European Union 2004, 2004Google Scholar
- 5.National Health and Family Planning Commission of the People’s Republic of China (2012) Quality requirements for whole blood and blood componentsGoogle Scholar
- 7.Balleisen L, Assmann G, Bailey J, Epping PH, Schulte H, van de Loo J (1985) Epidemiological study on factor VII, factor VIII and fibrinogen in an industrial population—II. Baseline data on the relation to blood pressure, blood glucose, uric acid, and lipid fractions. Thromb Haemost 54(3):721–723CrossRefGoogle Scholar
- 8.Balleisen L, Bailey J, Epping PH, Schulte H, van de Loo J (1985) Epidemiological study on factor VII, factor VIII and fibrinogen in an industrial population: I. Baseline data on the relation to age, gender, body-weight, smoking, alcohol, pill-using, and menopause. Thromb Haemost 54(2):475–479PubMedGoogle Scholar
- 10.Lowe GD, Rumley A, Woodward M, Morrison CE, Philippou H, Lane DA, Tunstall-Pedoe H (1997) Epidemiology of coagulation factors, inhibitors and activation markers: the Third Glasgow MONICA Survey. I. Illustrative reference ranges by age, sex and hormone use. Br J Haematol 97(4):775–784CrossRefGoogle Scholar
- 12.Wang Z, Liu H, Dou M, Du X, Hu J, Su N, Wang Y, Zhang R, Li C.The quality changes in fresh frozen plasma of the blood donors at high altitude. PLoS ONE 2017 12(4):e0176390Google Scholar
- 18.Rios DRA, Fernandes AP, Figueiredo RC, Guimaraes DAM, Ferreira CN, Silva E, Carvalho ACS, Gomes MG, Dusse KB (2012) LMS. Relationship between ABO blood groups and von Willebrand factor, ADAMTS13 and factor VIII in patients undergoing hemodialysis. J Thromb Thrombolysis 33:416–421CrossRefGoogle Scholar