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Journal of Thrombosis and Thrombolysis

, Volume 46, Issue 4, pp 528–533 | Cite as

Effect of remote ischaemic conditioning on platelet aggregation and platelet turnover

  • Nina Rise
  • Jacobina Kristiansen
  • Anne-Mette Hvas
  • Erik L. Grove
  • Morten Würtz
  • Søs Neergaard-Petersen
  • Steen Dalby Kristensen
Article

Abstract

Remote ischaemic conditioning (RIC) is a new beneficial treatment for patients with ST-elevation myocardial infarction. RIC may inhibit thrombus formation and, therefore, we investigated whether RIC affects platelet aggregation and turnover. 30 healthy male volunteers were subjected to intervention on day 1 (sham intervention, no aspirin), day 2 (RIC, no aspirin), and day 16 (RIC, treated 7 days with aspirin 75 mg/day). RIC was performed as four cycles of 5 min interchangeable inflation and deflation using an automated cuff. Blood samples were collected 5 min before, as well as 5 and 45 min after RIC. Platelet aggregation was measured by Multiplate® using collagen (COLtest), adenosine diphosphate (ADPtest), and arachidonic acid (ASPItest) as agonists. Platelet turnover was evaluated by flow cytometry. Serum thromboxane B2 was determined by ELISA to confirm aspirin compliance. We found no significant change in platelet aggregation at visit 1 (COLtest: p = 0.32; ADPtest: p = 0.24; ASPItest: p = 0.07), visit 2, except for ADP-induced platelet aggregation evaluated 5 min after RIC (COLtest: p = 0.39; ADPtest: p = 0.02; ASPItest: p = 0.39), or visit 3 (COLtest: p = 0.48; ADPtest: p = 0.61; ASPItest: p = 0.90). Platelet turnover was not influenced by RIC, neither on nor off aspirin (all p-values > 0.07). (1) RIC did not affect platelet aggregation in healthy young men. (2) RIC did not affect platelet turnover in healthy young men. (3) Aspirin did not influence the effect of RIC on platelet aggregation and turnover. (4) Future studies exploring the effect of RIC on platelet aggregation and turnover in patients with ischaemic heart disease are warranted.

Keywords

Aspirin Ischaemic conditioning Platelet aggregation Platelet function tests Platelet turnover 

Notes

Acknowledgements

We wish to thank laboratory technicians Vivi Bo Mogensen and Mai Stenulm Veirup, Department of Clinical Biochemistry, Aarhus University Hospital, Denmark for laboratory assistance.

Funding

The study was supported by The Danish Heart Association, Independent Research Fund Denmark, A.P. Møller Foundation, Helge Peetz og Verner Peetz og hustru Vilma Peetz Foundation, Murermester Lauritz P. Christensen og hustru Kirsten S. Christensens Foundation, Snedkermester Sophus Jacobsen & hustru Astrid Jacobsen Foundation, and Fonden af 17-12-198. The funding sources had no influence on the study design, collection, analysis or interpretation of the data, writing of the manuscript, or the decision to submit the manuscript for publication.

Compliance with ethical standards

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Nina Rise
    • 1
    • 2
  • Jacobina Kristiansen
    • 1
    • 2
  • Anne-Mette Hvas
    • 2
    • 3
  • Erik L. Grove
    • 1
    • 3
  • Morten Würtz
    • 1
  • Søs Neergaard-Petersen
    • 1
  • Steen Dalby Kristensen
    • 1
    • 3
  1. 1.Department of CardiologyAarhus University HospitalAarhusDenmark
  2. 2.Department of Clinical BiochemistryAarhus University HospitalAarhusDenmark
  3. 3.Department of Clinical Medicine, Faculty of HealthAarhus UniversityAarhusDenmark

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