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Redescription and first genetic characterisation of Procamallanus (Spirocamallanus) macaensis Vicente & Santos, 1972 (Nematoda: Camallanidae), including re-evaluation of the species of Procamallanus (Spirocamallanus) from marine fishes off Brazil

Abstract

Newly collected specimens of Procamallanus (Spirocamallanus) macaensis Vicente & Santos, 1972 from the intestine of Paralonchurus brasiliensis (Steindachner), off Rio de Janeiro, Brazil, are redescribed and genetically characterised. Additionally, all congeners deposited in the Coleção Helmintológica do Instituto Oswaldo Cruz (CHIOC) parasitic in marine fishes of the South Atlantic, including types of P. (S.) macaensis, were re-evaluated. The following features are described for the first time in P. (S.) macaensis: morphology and arrangement of cephalic structures, shape of deirids and location of phasmids. The position of the excretory pore, the number and arrangement of caudal papillae in males, the structure of the spicules and of tail end in both males and females are rectified. Most specimens deposited in the CHIOC identified as P. (S.) pereirai Annereaux, 1946 were transferred to P. (S.) macaensis and others were designated as Procamallanus (S.) sp. Procamallanus (S.) cruzi Guimarães, Cristófaro & Rodrigues, 1976 is considered a species inquirenda due to its poor description and the lack of match of its original description with the type-material re-examined. Moreover, several taxonomic problems were noted after observations of the specimens (mostly poorly preserved), including inadequate morphological reports as well as misidentifications. Phylogenies inferred using sequences of the SSU rDNA from camallanids (Nematoda: Camallanidae) mostly generated weakly supported clades; however, Camallanus Railliet & Henry, 1915 and Procamallanus Baylis, 1923 do not seem to be monophyletic. Based on the present results and the lack of molecular data, it would be pertinent to adopt the widely-used classification for the subgenera of Procamallanus.

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Acknowledgements

The authors are thankful to Tomáš Scholz from the Institute of Parasitology, Biology Centre of the Czech Academy of Sciences (IPCAS) and the Staff of the Laboratory of Electron Microscopy from the same institute (IPCAS; RVO: 60077344) and the Czech Science Foundation (project No. P505/12/G112) for the support and for covering costs of SEM observations at the IPCAS. Thanks are also due to Dr. Marcelo Knoff curator of the Coleção Helmintológica do Instituto Oswaldo Cruz (acronym CHIOC) and Daniela A. Lopes by the loan of the specimens analysed in the present study.

Funding

Carla J. Sardella was supported by a Master fellowship from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico do Brasil), Felipe B. Pereira was supported by a PNPD-CAPES Post-doctoral fellowship (Programa Nacional de Pós-Doutorado-Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-CAPES, Brazil) and José L. Luque was supported by a Research fellowship from CNPq.

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Correspondence to José L. Luque.

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All applicable institutional, national and international guidelines for the care and use of animals were followed.

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This article was registered in the Official Register of Zoological Nomenclature (ZooBank) as 13AFF550-ADC0-41B4-842E-B345DB51F877. This article was published as an Online First article on the online publication date shown on this page. The article should be cited by using the doi number. This is the Version of Record.

This article is part of the Topical Collection Nematoda.

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Sardella, C.J., Pereira, F.B. & Luque, J.L. Redescription and first genetic characterisation of Procamallanus (Spirocamallanus) macaensis Vicente & Santos, 1972 (Nematoda: Camallanidae), including re-evaluation of the species of Procamallanus (Spirocamallanus) from marine fishes off Brazil. Syst Parasitol 94, 657–668 (2017). https://doi.org/10.1007/s11230-017-9728-2

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Keywords

  • Excretory Pore
  • Anterior Extremity
  • Basal Ring
  • Buccal Capsule
  • Muscular Oesophagus