Ultrastructural aspects and molecular phylogeny of Auerbachia maamouni n. sp. (Myxosporea: Bivalvulida) from the gallbladder of Gnathanodon speciosus Forsskål (Actinopterygii: Carangidae) in the Red Sea
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A new myxosporean parasite, Auerbachia maamouni n. sp., infecting the gallbladder of the golden trevally Gnathanodon speciosus Forsskål, is described, based on morphology, ultrastructure, and small subunit (SSU) rRNA gene sequencing. Of 50 fish collected from the Red Sea in Jeddah, Saudi Arabia, five were found heavily infected with mature myxospores floating free in the bile. Mature spores are pyriform to club-shaped with smooth valves, and contain a single polar capsule with an S-shaped polar filament, arranged in 13–16 polar filament coils, oriented longitudinally, with an irregular distribution on the polar capsule matrix. Spores measure 15.8 (14–17) μm in total length in lateral view, 7.9 (6–9) μm in width in apical view, with spore body length of 6.2 ± 0.4 (5–7) µm. The ellipsoidal polar capsule has two adjusted lateral folds 7.6 (6–8) µm long and 2 (2–3) µm wide. The new species is distinguished from other species of the genus based on spore morphometry and molecular data. The phylogenetic tree constructed using Maximum Likelihood and Bayesian Inference analysis of SSU rDNA sequence data supported the phylogenetic position of A. maamouni n. sp. among the species of Auerbachia Meglitsch, 1968 sequenced to date. Analysis of the SSU rDNA sequence data also supported the assumption that Auerbachia is closely related to members of the genera Coccomyxa Léger & Hesse, 1907, Zschokkella Auerbach, 1910 and Myxidium Bütschli, 1882, whose members inhabit the gallbladder of marine teleost fishes.
KeywordsPolar Capsule Polar Filament Shell Valve Spore Body Marine Teleost Fish
We extend our appreciation to the Dean of Scientific Research, King Saud University, for funding the work through the research group project number RG-004.
This study was supported by project RG-004 of the Dean of Scientific Research, King Saud University, Saudi Arabia.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable institutional, national and international guidelines for the care and use of animals were followed.
- Hall, T. A. (1999). Bioedit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symposium, 41, 95–98.Google Scholar
- Laroche, W. A., Smith-Vaniz, W. F., & Richardson, S. L. (1984). Carangidae: development. In: Moser, H. G., Richards, W. J., Cohen, D. M., Fahay, M. P., Kendall, A. W. & Richardson, S. L. (Eds), Ontogeny and systematics of fishes. American Society for ichthyology and herpetology special publication 1. Lawrence: Allen Press, pp. 510–522.Google Scholar
- Lom, J., & Dyková, I. (1992). Protozoan parasites of fishes. In: Developments in Aquaculture and Fisheries Science (Book 26). Amsterdam, Netherlands: Elsevier, pp. 159–235.Google Scholar
- Lom, J., & Dyková, I. (1993). Scanning electron microscopic revision of common species of the genus Chloromyxum (Myxozoa: Myxosporea) infecting European freshwater fishes. Folia Parasitologica., 40, 161–174.Google Scholar
- Lom, J., & Noble, E. R. (1984). Revised classification of the Class Myxosporea Büetschli, 1881. Folia Parasitologica., 31, 193–205.Google Scholar
- Lom, J., Noble, E. R., & Laird, M. (1975). Myxosporida from the deep-sea fish, Macrourus berglax, off Newfoundland and Iceland. Folia Parasitologica, 22, 105–109.Google Scholar
- Mansour, L., Abdel-Baki, A. S., Al-Qahtani, H. A., & Al-Quraishy, S. (2015). Morphological and molecular aspects of Ceratomyxa mehlhorni n. sp., a parasite of the golden trevally Gnathanodon speciosus in the Arabian Gulf off the Saudi Arabian coast, with data on its seasonal prevalence. Parasitology Research, 114, 3783–3789.CrossRefPubMedGoogle Scholar
- Meglitsch, P. A. (1968). Some coelozoic Myxosporida from New Zealand Fishes II. On a new genus of Myxosporida, Auerbachia. Proceedings of the Iowa Academy of Science, 75, 397–401.Google Scholar
- Molnár, K. (1991). Comments on the host, organ and tissue specificity of fish myxosporeans and on the types of their intrapiscine development. Parasitologia Hungarica, 27, 5–20.Google Scholar
- Narasimhamurti, C. C., Kalavati, C., Anuradha, I., & Padma Dorothy, K. (1990). Studies on the protozoan parasites of deepwater fishes from the Bay of Bengal. In: Proceedings I. Workshop on Scientific Results of FORV Sagar Sampada 5–7th June, 1989, pp. 325–336.Google Scholar
- Padma-Dorothy, K., Kalavati, C., & Vaidehi, L. (1998). Three new species of myxozoans from teleosts of Bay of Bengal. Rivista di Parassitologia, 15, 67–74.Google Scholar
- Sarkar, N. K. (2006). Auerbachia hepatica sp. n. (Auerbachiidae Evdokimova, 1973) and Sinuolinea renalis sp. n. (Sinuolineidae Shulman, 1959) from the teleosts of coastal water of Bay of Bengal. Uttar Pradesh Journal of Zoology, 26, 319–323.Google Scholar
- Walter, T., Palm, H. W., Piepiorka, S., & Ruckert, S. (2002). Parasites of the Antarctic rattail Macrourus whitsoni (Regan, 1913) (Macrouridae, Gadiformes). Polar Biology, 25, 633–640.Google Scholar
- Whipps, C. M., Adlard, R. D., Bryant, M. S., Lester, R. J., Findlay, V., & Kent, M. L. (2003). First report of three Kudoa species from eastern Australia: Kudoa thyrsites from mahi mahi (Coryphaena hippurus), Kudoa amamiensis and Kudoa minithyrsites n. sp. from sweeper (Pempheris ypsilychnus). Journal of Eukaryotic Microbiology, 50, 215–219.CrossRefPubMedGoogle Scholar
- Zubchenko, A. V. (1985). Use of parasitological data in studies of the local groupings of Rock Grenadier, Coryphaenoides rupestris Gunner. National Oceanographic and Atmospheric Technical Report NMFS, 25, 19–23.Google Scholar