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Systematic Parasitology

, Volume 94, Issue 1, pp 123–131 | Cite as

Ultrastructural aspects and molecular phylogeny of Auerbachia maamouni n. sp. (Myxosporea: Bivalvulida) from the gallbladder of Gnathanodon speciosus Forsskål (Actinopterygii: Carangidae) in the Red Sea

  • Lamjed Mansour
  • Carlos Azevedo
  • Ângela Alves
  • Saleh Al-Quraishy
  • Abdel-Azeem S. Abdel-Baki
Article
  • 208 Downloads

Abstract

A new myxosporean parasite, Auerbachia maamouni n. sp., infecting the gallbladder of the golden trevally Gnathanodon speciosus Forsskål, is described, based on morphology, ultrastructure, and small subunit (SSU) rRNA gene sequencing. Of 50 fish collected from the Red Sea in Jeddah, Saudi Arabia, five were found heavily infected with mature myxospores floating free in the bile. Mature spores are pyriform to club-shaped with smooth valves, and contain a single polar capsule with an S-shaped polar filament, arranged in 13–16 polar filament coils, oriented longitudinally, with an irregular distribution on the polar capsule matrix. Spores measure 15.8 (14–17) μm in total length in lateral view, 7.9 (6–9) μm in width in apical view, with spore body length of 6.2 ± 0.4 (5–7) µm. The ellipsoidal polar capsule has two adjusted lateral folds 7.6 (6–8) µm long and 2 (2–3) µm wide. The new species is distinguished from other species of the genus based on spore morphometry and molecular data. The phylogenetic tree constructed using Maximum Likelihood and Bayesian Inference analysis of SSU rDNA sequence data supported the phylogenetic position of A. maamouni n. sp. among the species of Auerbachia Meglitsch, 1968 sequenced to date. Analysis of the SSU rDNA sequence data also supported the assumption that Auerbachia is closely related to members of the genera Coccomyxa Léger & Hesse, 1907, Zschokkella Auerbach, 1910 and Myxidium Bütschli, 1882, whose members inhabit the gallbladder of marine teleost fishes.

Keywords

Polar Capsule Polar Filament Shell Valve Spore Body Marine Teleost Fish 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgement

We extend our appreciation to the Dean of Scientific Research, King Saud University, for funding the work through the research group project number RG-004.

Funding

This study was supported by project RG-004 of the Dean of Scientific Research, King Saud University, Saudi Arabia.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable institutional, national and international guidelines for the care and use of animals were followed.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Lamjed Mansour
    • 1
    • 2
  • Carlos Azevedo
    • 1
    • 3
    • 4
  • Ângela Alves
    • 3
  • Saleh Al-Quraishy
    • 1
  • Abdel-Azeem S. Abdel-Baki
    • 1
    • 5
  1. 1.Zoology Department, College of SciencesKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Unité de Recherche de Biologie intégrative et Ecologie évolutive et Fonctionnelle des Milieux Aquatiques, Département de Biologie, Faculté des Sciences de TunisUniversité De Tunis El ManarTunisTunisia
  3. 3.Laboratory of Cell Biology, Institute of Biomedical Sciences (ICBAS/UP)University of PortoPortoPortugal
  4. 4.Laboratory of Animal Pathology, Interdisciplinary Centre of Marine and Environmental Research (CIIMAR/UP)University of PortoPortoPortugal
  5. 5.Zoology Department, Faculty of ScienceBeni-Suef UniversityBeni-SuefEgypt

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