Systematic Parasitology

, Volume 91, Issue 1, pp 71–79 | Cite as

Description of Myxobolus balatonicus n. sp. (Myxozoa: Myxobolidae) from the common carp Cyprinus carpio L. in Lake Balaton

Article

Abstract

Myxobolus balatonicus n. sp. was detected in the gill filaments of the common carp Cyprinus carpio L. collected in Lake Balaton, Hungary. Its oval plasmodia measuring 600–800 × 300–400 µm were located intravasally in the filamental arteries. The spores measured 11.2 ± 0.92 × 9.5 ± 0.41 × 7.4 ± 0.33 µm and had two equal polar capsules with six filamental turns. Both morphology and DNA sequence analysis revealed that M. balatonicus n. sp. is distinct from the ten species of Myxobolus Bütschli, 1882 described from the European common carp and the 21 species described from the Asian common carp subspecies. Phylogenetic analysis placed M. balatonicus n. sp. in a clade of gill-infecting myxobolids.

Notes

Acknowledgements

This study was supported by the OTKA K 100132 and KTIA-AIK-12-1-2013-0017 projects.

References

  1. Akhmerov, A. K. (1960). Myxosporidia of fishes of the Amur River Basin. Rybnoe Khozyaistvo Vnutrikh Vodoemov Latviiskoi SSR, 5, 239–308 (In Russian).Google Scholar
  2. Antychowicz, J., & Reichert, M. (1987). Occurrence of Myxobolus encephalicus (Muslow 1911) in Poland: Possible relationship between the parasite infection and clinical symptoms in common carp (Cyprinus carpio). Bulletin of the Veterinary Institute, Puławy, 49, 35–39.Google Scholar
  3. Barta, J. R., Martin, D. S., Libetator, P. A., Dashkevicz, M., Anderson, J. W., et al. (1997). Phylogenetic relationships among eight Eimeria species infecting domestic fowl inferred using complete small subunit ribosomal DNA sequences. Journal of Parasitology, 83, 262–271.CrossRefPubMedGoogle Scholar
  4. Chen, Q. L., & Ma, C. L. (1998). Myxozoa, Myxosporea. Fauna Sinica. Beijing: Science Press, pp. 292–528 (In Chinese).Google Scholar
  5. Cirkovic, M., Milosevic, N., Markovic, M., & Potkonjak, A. (2010). Brain myxoboliasis of common carp. Bulgarian Journal of Agricultural Sciences, 16, 263–265.Google Scholar
  6. Donec, Z. S., & Shulman, S. S. (1984). Parasitic Protozoa. In: Bauer O. N. (Ed.) Key to parasites of freshwater fish of the USSR. Volume 1. Leningrad: Nauka, 426 pp (In Russian).Google Scholar
  7. Dyková, I., & Lom, J. (1988). Review of pathogenic myxosporeans in intensive culture of carp (Cyprinus carpio) in Europe. Folia Parasitologica, 35, 289–307.Google Scholar
  8. Dyková, I., Lom, J., & Cirkovic, M. (1986). Brain myxobolosis of common carp (Cyprinus carpio) due to Myxobolus encephalicus. Bulletin of the European Association of Fish Pathologists, 6, 10–11.Google Scholar
  9. Eiras, J. C., Molnár, K., & Lu, Y. S. (2005). Synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea: Myxobolidae). Systematic Parasitology, 61, 1–46.CrossRefPubMedGoogle Scholar
  10. Eiras, J. C., Zhang, J., & Molnár, K. (2014). Synopsis of the species of Myxobolus Bütschli, 1882 (Myxozoa: Myxosporea, Myxobolidae) described between 2005 and 2013. Systematic Parasitology, 88, 11–36.CrossRefPubMedGoogle Scholar
  11. Eszterbauer, E. (2004). Genetic relationship among gill-infecting Myxobolus species (Myxosporea) of cyprinids: molecular evidence of importance of tissue-specificity. Diseases of Aquatic Organisms, 58, 35–40.CrossRefPubMedGoogle Scholar
  12. Eszterbauer, E., & Székely, C. (2004). Molecular phylogeny of the kidney parasitic Sphaerospora renicola from common carp (Cyprinus carpio) and Sphaerospora sp. from goldfish (Carassius auratus auratus). Acta Veterinaria Hungarica, 52, 469–478.CrossRefPubMedGoogle Scholar
  13. Froufe, E., Magyari, I., Lehoczky, I., & Weiss, S. (2002). mtDNA sequence data support an Asian ancestry and single introduction of the common carp into the Danube Basin. Journal of Fish Biology, 61, 301–304.CrossRefGoogle Scholar
  14. Hallett, S. L., & Diamant, A. (2001). Ultrastructure and small-subunit ribosomal DNA sequence of Henneguya lesteri n. sp. (Myxosporea), a parasite of sand whiting Sillago analis (Sillaginidae) from the coast of Queensland, Australia. Diseases of Aquatic Organisms, 46, 197–212.CrossRefPubMedGoogle Scholar
  15. Huang, M., Liu, Y., Jia, L., Zhai, Y., Deng, Q., & Gu, Z. M. (2014). Supplementary studies on Myxobolus tsangwuensis Chen, 1954 (Myxozoa: Myxosporea) infecting the gills of common carp Cyprinus carpio (L.): Molecular and histological data. Acta Parasitologica, 59, 653–660.CrossRefPubMedGoogle Scholar
  16. Liu, Y., Whipps, C. M., Gu, Z. M., Huang, M. J., He, C., Yang, H. L., & Molnár, K. (2013). Myxobolus musseliusae (Myxozoa: Myxobolidae) from the gills of common carp Cyprinus carpio and revision of Myxobolus dispar recorded in China. Parasitology Research, 112, 289–296.CrossRefPubMedGoogle Scholar
  17. Lom, J., & Molnár, K. (1983). Myxobolus basilamellaris n. sp. (Myxozoa, Myxosporea) parasite of the gills of common carp (Cyprinus carpio L.). Folia Parasitologica, 30, 1–3.Google Scholar
  18. Molnár, K. (2000). Myxobolus intrachondrealis sp. n. (Myxosporea: Myxobolidae), a parasite of the gill cartilage of the common carp. Cyprinus carpio. Folia Parasitologica, 47, 167–171.CrossRefGoogle Scholar
  19. Molnár, K. (2002a). Redescription and histopathology of Myxobolus cyprinicola Reuss, 1906, an intestinal parasite of the common carp (Cyprinus carpio L.). Acta Protozoologica, 41, 279–283.Google Scholar
  20. Molnár, K. (2002b). Site preference of myxosporeans in the gill. Diseases of Aquatic Organisms, 48, 197–207.CrossRefPubMedGoogle Scholar
  21. Molnár, K. (2009). Data on the parasite fauna of the European common carp Cyprinus carpio carpio and Asian common carp Cyprinus carpio haematopterus support an Asian ancestry of the species. Aquaculture, Aquarium, Conservation & Legislation—International Journal of the Bioflux Society, 2, 391–400.Google Scholar
  22. Molnár, K., & Kovács-Gayer, E. (1985). The pathogenicity and development within the host fish of Myxobolus cyprini Doflein, 1898. Parasitology, 90, 549–555.CrossRefGoogle Scholar
  23. Molnár, K., & Székely, C. (1995). Parasitological survey of some important fish species of Lake Balaton. Parasitologia Hungarica, 28, 63–82.Google Scholar
  24. Molnár, K., El-Mansy, A., Székely, C., & Baska, F. (1999). Development of Myxobolus dispar Thelohan, 1895 (Myxosporea: Myxobolidae) in an oligochaete alternate host Tubifex tubifex (Müller). Folia Parasitologica, 46, 15–21.Google Scholar
  25. Molnár, K., Eszterbauer, E., Székely, C., Dán, Á., & Harrach, B. (2002). Morphological and molecular biological studies on intramuscular Myxobolus spp. of cyprinid fish. Journal of Fish Diseases, 25, 643–652.CrossRefGoogle Scholar
  26. Molnár, K., Cech, G., & Székely, C. (2008). Infection of the heart of the common bream, Abramis brama (L.) with Myxobolus s.l. dogieli (Myxozoa, Myxobolidae). Journal of Fish Diseases, 31, 613–620.CrossRefPubMedGoogle Scholar
  27. Ogawa, K., Delgahapitiya, K. P., Furuta, T., & Wakabayashi, H. (1992). Histological studies on the host response to Myxobolus artus Akhmerov, 1960 (Myxozoa, Myxobolidae) infection in the skeletal muscle of carp, Cyprinus carpio L. Journal of Fish Biology, 41, 363–371.CrossRefGoogle Scholar
  28. Székely, C., & Molnár, K. (1997). Preliminary survey of the parasite fauna of some important fish species in the Upper-Reservoir of the Kis-Balaton System. Parasitologia Hungarica, 29–30, 45–54.Google Scholar
  29. Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis, version 6.0. Molecular Biology and Evolution, 30, 2725–2729.CrossRefPubMedCentralPubMedGoogle Scholar
  30. Thompson, J. D., Higgins, D. G., & Gibson, T. J. (1994). CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.CrossRefPubMedCentralPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  1. 1.Institute for Veterinary Medical Research, Centre for Agricultural ResearchHungarian Academy of SciencesBudapestHungary

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