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Ultrastructural features of the tegumental surface of a new metacercaria, Nematostrigea sp. (Trematoda: Strigeidae), with a search for potential taxonomically informative characters

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Abstract

The tegumental surface of a new strigeid metacercaria, Nematostrigea sp., which is a parasite of the freshwater fish Channa gachua (Hamilton) in central Vietnam, is described for the first time using scanning (SEM) and transmission (TEM) electron microscopy. In addition to the general tegumental surface in various parts of the body, details of the surface of the suckers, lappets and holdfast organ are presented, as are variations in the form and distribution of the body spines. As good taxonomic criteria are few in diplostomoid metacercariae at both specific and generic levels, a number of the ultrastructural features revealed may prove to represent taxonomically informative characters. These include the presence of: two rings of dome-shaped papillae localised at different levels on the rim of the oral sucker, a single ring of ciliated papillae on the inner margin of the ventral sucker and a band of dome-shaped papillae along the lateral margins of the broad body-fold in the ventral forebody; an unarmed oral sucker and anteroventral surface of the forebody, although the latter bears protuberant secretory pores; an armed ventral sucker covered by six-pointed spines, except on its rim; multi-pointed spines along the dorsal and ventral sides of the forebody, with the number of their teeth increasing posteriorly; multi-pointed spines on the forebody which gradually transform into single-pointed, more widely distributed spines on the hindbody, disappearing completely at posterior end of the body; the surface of the lappets with a particular distribution of pores leading to three types of secretory glands and three topographical modifications (areas where the surface is smooth, bears digitiform processes or bears recurved, dagger-shaped spines); and the surface of the holdfast organ which is covered with densely packed, straight or slightly curved, simple spines on its lateral surface but is smooth medially.

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Acknowledgements

The authors would like to thank the workers of the Coastal Department of the Russian-Vietnamese Tropical Centre in Vietnam for their continuous support and the staff of the Centre of Electron Microscopy, Institute of Biology of Inland Waters, Russia. The present study was supported by the Russian Foundation for Fundamental Research (project no. 09-04-00342a).

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Correspondence to Larisa G. Poddubnaya.

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Poddubnaya, L.G., Mishina, E., Zhokhov, A.E. et al. Ultrastructural features of the tegumental surface of a new metacercaria, Nematostrigea sp. (Trematoda: Strigeidae), with a search for potential taxonomically informative characters. Syst Parasitol 75, 59 (2010). https://doi.org/10.1007/s11230-009-9207-5

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Keywords

  • Ventral Sucker
  • Oral Sucker
  • Scanning Electron Microscopical Measurement
  • Simple Spine
  • Sensory Papilla