Advertisement

Systematic Parasitology

, Volume 61, Issue 2, pp 143–156 | Cite as

Corynosoma australe Johnston, 1937 and C. cetaceum Johnston & Best, 1942 (Acanthocephala: Polymorphidae) from marine mammals and fishes in Argentinian waters: allozyme markers and taxonomic status

  • Norma H. SardellaEmail author
  • Simonetta Mattiucci
  • Juan T. Timi
  • Ricardo O. Bastida
  • Diego H. Rodríguez
  • Giuseppe Nascetti
Article

Abstract

Genetic and morphological studies were carried out on acanthocephalans belonging to Corynosoma Lühe, 1904 and referable to the species C. cetaceum Johnston & Best, 1942 and C. australe Johnston, 1937, which were recovered from both definitive and intermediate hosts in Argentinian waters. The aims were to estimate the level of genetic differentiation between the two taxa at any stage of their life-cycle, to provide genetic (allozyme) markers for their recognition and to analyse the systematic status of both taxa. Acanthocephalans were collected from the stomach and intestine of Arctocephalus australis (Zimmerman), the intestine of Mirounga leonina (Linnaeus) and the stomach of Pontoporia blainvillei Gervais & D’Orbigny (definitive hosts) in Argentinian waters. Alternative alleles at all the 13 enzymatic loci studied were observed for C. australe and C. cetaceum. The specimens from the stomach of both P. blainvillei and A. australis were identified, on the basis of the great number of diagnostic loci found, as C. cetaceum; those from intestine of both A. australis and M. leonina as C. australe. A high level of genetic differentiation (DNei=∞: INei = 0.00) between the two taxa was found, suggesting a generic distinction between the two species. Cystacanths of the two species from the body-cavity of the fish Cynoscion guatucupa (Cuvier) collected from the same geographical area were identified genetically. Morphological patterns, such as the number of hooks and hook rows on the proboscis, the distribution of somatic and genital armature, and other morphometric and meristic differences, in addition to ecological data, enabled the identification of these two species at cystacanth, juvenile and adult stages. However, a number of morphological and morphometric features of the Argentinian material were different to those of C. australe and C. cetaceum described from other regions of the world.

Keywords

Genetic Differentiation Systematic Status Adult Stage Intermediate Host Marine Mammal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aho, J.M., Mulvei, M., Jacobson, K.C., Esch, G.W. 1992Genetic differentiation among congeneric acanthocephalans in the yellow-bellied slider turtleJournal of Parasitology78974981Google Scholar
  2. Amin, O.M. 1985ClassificationCrompton, D.W.T.Nickol, B.B. eds. Biology of the AcanthocephalaCambridge University PressCambridge2772Google Scholar
  3. Amin, O.M. 1992Review of the genus Polymorphus Lühe, 1911 (Acanthocephala: Polymorphidae), with the synonymization of Hexaglandula Petrochenko, 1950, and Subcorynosoma Hoklova, 1967, and a key to the speciesQatar University Science Journal12115123Google Scholar
  4. Andrews, R.H., Chilton, N.B. 1999Multilocus allozyme electrophoresis: a valuable technique for providing answers to problems in parasite systematicsInternational Journal for Parasitology29213253CrossRefPubMedGoogle Scholar
  5. Aznar, F.J., Raga, J.A., Corcuera, J., Monzón, F. 1994aHelminths as biological tags for franciscana (Pontoporia blainvillei) (Cetacea, Pontoporidae) in Argentinian and Uruguayan watersMammalia59427435Google Scholar
  6. Aznar, F.J., Balbuena, J.A., Raga, J.A. 1994bHelminth communities of Pontoporia blainvillei (Cetacea, Pontoporidae) in Argentinian watersCanadian Journal of Zoology72702706Google Scholar
  7. Aznar, F.J., Bush, A.O., Raga, J.A. 1999aPolymorphus arctocephali Smales, 1986, a synonym of Corynosoma cetaceum Johnston, Best, 1942 (Acanthocephala: Polymorphidae)Systematic Parasitology446172CrossRefGoogle Scholar
  8. Aznar, F.J., Bush, A.O., Fernández, M., Raga, J.A. 1999bConstructional morphology and mode of attachment of the trunk of Corynosoma cetaceum (Acanthocephala: Polymorphidae)Journal of Morphology241237249CrossRefGoogle Scholar
  9. Aznar, F.J., Bush, A.O, Balbuena, J.A., Raga, J.A. 2001Corynosoma cetaceum in the stomach of franciscanas, Pontoporia blainvillei (Cetacea): an exceptional case of habitat selection by an acanthocephalanJournal of Parasitology87536541PubMedGoogle Scholar
  10. Aznar, F.J., Berón-Vera, B., Crespo, E., Raga, J.A. 2002aPresence of genital spines in a male Corynosoma cetaceum Johnston, Best, 1942 (Acanthocephala)Journal of Parasitology88403404Google Scholar
  11. Aznar, F.J., Bush, A.O., Raga, J.A. 2002bReduction and variability of trunk spines in the acanthocephalan Corynosoma cetaceum: the role of physical constraints on attachmentInvertebrate Biology121104114Google Scholar
  12. Aznar, F.J., Cappozzo, H.L., Taddeo, D., Montero, F.E., Raga, J.A. 2004Recruitment, populations structure, and habitat selection of Corynosoma australe (Acanthocephala) in South American fur seals, Arctocephalus australis, from UruguayCanadian Journal of Zoology82741748CrossRefGoogle Scholar
  13. Cremonte, F., Sardella, N.H. 1997The parasitofauna of Scomber japonicus Houttuyn, 1782 (Pisces: Scombridae) in two zones of the Argentine SeaFisheries Research3119CrossRefGoogle Scholar
  14. Dailey, M.D., Brownell, R.L.,Jr 1972A checklist of marine mammal parasitesRidgway, S.H. eds. Mammals of the sea. Biology and medicineCharles C. ThomasSpringfield, Illinois528589Google Scholar
  15. Dans, S.L., Reyes, L.M., Pedraza, S.M, Raga, J.A., Crespo, E.A. 1999Gastrointestinal helminths of the dusky dolphin, Lagenorhynchus obscurus (Gray, 1828), off Patagonia, in the Southwest AtlanticMarine Mammal Science15649660Google Scholar
  16. De Buron, I., Renaud, F., Euzet, L. 1986Speciation and specificity of acanthocephalans. Genetic and morphological studies of Acanthocephaloides geneticus sp.nov. parasitizing Arnoglossus laterna (Bothidae) from the Mediterranean littoral (Sète – France)Parasitology92165171Google Scholar
  17. Delyamure, S.L. (1968) Helminthofauna of marine mammals (ecology and phylogeny). Jerusalem: Israel Program for Scientific Translation, 522 pp. (English translation of Russian original (1955) [The helminthofauna of marine mammals of the world their ecology and phylogeny.] Moscow: Nauka, 517 pp.)Google Scholar
  18. García-Varela, M., Aznar, F.J., Pérez-Ponce de León, G., Piñero, D. & Laclette, J.P. (2005) Molecular phylogeny of Corynosoma Lühe, 1904 (Acanthocephala) based on 5.8S and internal transcribed spacer sequences. Journal of Parasitology, in pressGoogle Scholar
  19. George-Nascimento, M.F., Marín, S.L. 1992Efecto de dos especies hospedadoras, el lobo fino austral Arctocephalus australis (Zimmerman) y el lobo marino común Otaria byronia (Blainville) (Carnivora; Otariidae), sobre la morfología y la fecundidad de Corynosoma sp. (Acanthocephala; Polymorphidae) en UruguayRevista Chilena de Historia Natural65183193Google Scholar
  20. Ivanov V.A. (1996) Ecología de los helmintos parásitos de peces marinos. Tesis Doctoral. Universidad Nacional de La Plata, Facultad de Ciencias Naturales y Museo, 176 ppGoogle Scholar
  21. Johnston, T.H. 1937Entozoa from the Australian hair sealProceedings of the Linnean Society of New South Wales62916Google Scholar
  22. Johnston, T.H., Best, E.W. 1942Australian Acanthocephala. No. 3Transactions of the Royal Society of South Australia66250254Google Scholar
  23. Kagei, N., Tobayama, T., Nagasaki, Y. 1976On the helminthum of franciscana, Pontoporia blainvilleiScientific Report of the Whales Research Institute28161166Google Scholar
  24. Knoff, M., São Clemente, S.C., Pinto, R.M., Gomes, D.C. 2001Digenea and acanthocephala of elasmobranch fished from the Southern coast of BrazilMemórias do Instituto Oswaldo Cruz9610951101PubMedGoogle Scholar
  25. Mattiucci, S., Nascetti, G., Cianchi, R., Paggi, L., Arduino, P., Margolis, L., Brattey, J., Webb, S., D’Amelio, S., Orecchia, P., Bullini, L. 1997Genetic and ecological data on the Anisakis simplex complex, with evidence for a new species (Nematoda, Ascaridoidea, Anisakidae)Journal of Parasitology83401416CrossRefPubMedGoogle Scholar
  26. Mattiucci, S., Paggi, L., Nascetti, G., Abollo, E., Webb, S., Pascual, S., Cianchi, R., Bullini, L. 2001Genetic divergence and reproductive isolation between Anisakis brevispiculata and Anisakis physeteris (Nematoda: Anisakidae)International Journal for Parasitology31914CrossRefPubMedGoogle Scholar
  27. Mattiucci, S., Cianchi, R., Nascetti, G., Paggi, L., Sardella, N., Timi, J., Webb, S., Bastida, R., Rodriguez, D., Bullini, L. 2003Genetic evidence for two sibling species within Contracaecum ogmorhini Johnston et Mawson, 1941 (Nematoda: Anisakidae) from otariid seals of boreal and austral regionsSystematic Parasitology541323CrossRefPubMedGoogle Scholar
  28. Morini, E.G., & Boero, J.J. (1960) Corynosoma otariae n. sp. (Acanthocephala; Polymorphidae) parásito de un lobo marino (Otaria flavescens). Actas y Trabajos 1° Congreso Sudamericano de Zoología, La Plata, 1959, pp. 229–234Google Scholar
  29. Nascetti, G., Paggi, L., Orecchia, P., Smith, J., Mattiucci, S., Bullini, L. 1986Electrophoretic studies on the Anisakis simplex complex (Ascaridida: Anisakidae) from Mediterranean and North-East AtlanticInternational Journal for Parasitology16633640CrossRefPubMedGoogle Scholar
  30. Nascetti, G., Bullini, L., Cianchi, R., Paggi, L., Orecchia, P., Mattiucci, S., Berland, B. 1990Genetic relationships among anisakid species belonging to the genera Contracaecum and PhocascarisBulletin de la Société Française de Parasitologie8261Google Scholar
  31. Nascetti, G., Cianchi, R., Matiucci, S., D’Amelio, S., Orecchia, P., Paggi, L., Brattey, J., Berland, B., Smith, J.W., Bullini, L. 1993Three sibling species within Contracaecum osculatum (Nematoda, Ascaridida, Ascaridoidea) from the Atlantic Arctic-Boreal region: reproductive isolation and host preferencesInternational Journal for Parasitology23105120CrossRefPubMedGoogle Scholar
  32. Nei, M. 1972Genetic distance between populationsThe American Naturalist106283292CrossRefGoogle Scholar
  33. Nickol, B.B., Crompton, D.W.T., Searle, D.W. 1999Reintroduction of Profilicollis Meyer, 1931, as a genus in Acanthocephala: significance of the intermediate hostJournal of Parasitology85716718PubMedGoogle Scholar
  34. Nickol, B.B., Helle, E., Valtonen, E.T. 2002aCorynosoma magdaleni in gray seals from the Gulf of Bothnia, with emended descriptions of Corynosoma strumosum and Corynosoma magdaleniJournal of Parasitology8812221229Google Scholar
  35. Nickol, B.B., Heard , R.W., Smith, N.F. 2002bAcanthocephalans from crabs in the southeastern U.S., with the first intermediate hosts known for Arhythmorhynchus frassoni and Hexaglandula corynosomaJournal of Parasitology887983Google Scholar
  36. Pereira , J.,Jr, Neves, L.F.M. 1993Corynosoma australe Johnston, 1937 (Acanthocephala, Polymorphidae) em Micropogonias furnieri (Desmarest, 1823) (Perciformes, Sciaenidae) de litoral do Rio Grande do SulComunicações do Museu de Ciências e Tecnologia da PUCRS, Série Zoologia65161Google Scholar
  37. Sardella, N.H., Timi, J.T. 1996Parasite communities of Merluccius hubbsi from the Argentinean-Uruguayan Common Fishing ZoneFisheries Research278188CrossRefGoogle Scholar
  38. Sardella, N.H., Etchegoin, J.A., Martorelli, S.R. 1995Las comunidades parasitarias de Micropogonias furnieri (corvina) en ArgentinaBoletín del Instituto Oceanográfico (Univ. de Oriente - Venezuela)344147Google Scholar
  39. Sardella, N.H., Avendaño, M.F., Timi, J.T. 1998Parasites communities of Genypterus blacodes and G. brasiliensis (Pisces: Ophidiidae) from ArgentinaHelminthologia35209218Google Scholar
  40. Schmidt, G.D. 1973Resurrection of Southwellina Witenberg, 1932, with a description of Southwellina dimorpha sp. n., and a key to genera in Polymorphidae (Acanthocephala)Journal of Parasitology59299305PubMedGoogle Scholar
  41. Schmidt, G.D. 1975Andracantha, a new genus of Acanthocephala (Polymorphidae) from fish-eating birds, with descriptions of three speciesJournal of Parasitology61615620PubMedGoogle Scholar
  42. Schmidt, G.D., Dailey, M.D. 1971Zoogeography and the generic status of Polymorphus (Polymorphus) cetaceum (Johnston, Best, 1942) comb. n. (Acanthocephala)Proceedings of the Helminthological Society of Washington38137Google Scholar
  43. Smales, L.R. 1986Polymorphidae (Acanthocephala) from Australian mammals with description of two new speciesSystematic Parasitology891100CrossRefGoogle Scholar
  44. Suriano, D.M. 1966Estudio de la fauna de “Micropogon opercularis” en relación con problemas zoogeográficos del Atlántico SurComunicaciones del Museo Argentino de Ciencias Naturales ‘‘Bernardino Rivadavia’’, Parasitología13147Google Scholar
  45. Swofford D.L., Selander R.B. (1989) BYOSYS-1, a computer program for the analysis of allelic variation in population genetics and biochemical systematics, version 1.7. Champaign: Illinois, Natural History Survey, 31 ppGoogle Scholar
  46. Szidat L. (1949) Los parásitos del róbalo (Eleginops maclovinus Cuv. & Val.). 1° Congreso Nacional de Pesquerías Marítimas e Industrias Derivadas, Mar del Plata, pp. 235–269Google Scholar
  47. Szidat, L. 1969Los parásitos de la “palometa” Parona signata (Jenyns, 1842) Berg, 1895, y su aplicación a problemas zoogeográficos del Atlántico SurNeotrópica15125131Google Scholar
  48. Tanzola, R.D., Guagliardo, S.E. 2000Helminth fauna of the Argentine conger, Conger orbignyanus (Pisces: Anguilliformes)Helminthologia37229232Google Scholar
  49. Tanzola, R.D., Guagliardo, S.E., Brizzola, S.M., Arias, M.B. 1997Helminth fauna of Porichthys porosissimus (Pisces: Batrachoidiformes) in the estuary of Bahía Blanca ArgentinaHelminthologia34221227Google Scholar
  50. Timi, J.T. 2003Parasites of Argentine anchovy, Engraulis anchoita, in the Southwest Atlantic, latitudinal patterns and their use for discrimination of host populationsJournal of Fish Biology6390107CrossRefGoogle Scholar
  51. Väinöla, R., Valtonen, E.T., Gibson, D.I. 1994Molecular systematics in the acanthocephalan genus Echinorhynchus (sensu lato) in northern EuropeParasitology108105114PubMedGoogle Scholar
  52. Zdzitowiecki, K. 1984aSome antarctic acanthocephalans of the genus Corynosoma parasitizing Pinnipedia, with descriptions of three new speciesActa Parasitologica Polonica29359377Google Scholar
  53. Zdzitowiecki, K. 1984bRedescription of Corynosoma hamanni (Linstow, 1892) and description of C. pseudohamanni sp. n. (Acanthocephala) from the environs of the South Shetlands (Antarctic)Acta Parasitologica Polonica29379393Google Scholar
  54. Zdzitowiecki, K. 1989New data on the morphology and distribution of two acanthocephalans, Andracantha baylisi (Zdzitowiecki, 1986) comb. n. and Corynosoma australe Johnston, 1937Acta Parasitologica Polonica34167172Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Norma H. Sardella
    • 1
    Email author
  • Simonetta Mattiucci
    • 2
  • Juan T. Timi
    • 1
    • 3
  • Ricardo O. Bastida
    • 3
    • 4
  • Diego H. Rodríguez
    • 3
    • 4
  • Giuseppe Nascetti
    • 5
  1. 1.Laboratorio de Parasitología, Departamento de Biología, Facultad de Ciencias Exactas y Naturales Universidad Nacional de Mar del PlataMar del PlataArgentina
  2. 2.Department of Public Health Sciences, (Section of Parasitology)University of Rome “La Sapienza”RomeItaly
  3. 3.Consejo Nacional de Investigaciones Científicas y Técnicas(CONICET)Mar del PlataArgentina
  4. 4.Laboratorio de Mamíferos Marinos, Departamento de Ciencias Marinas, Facultad de Ciencias Exactas y NaturalesUniversidad Nacional de Mar del PlataMar del PlataArgentina
  5. 5.Department of Ecology and Sustainable Economic Development (DECOS)Tuscia UniversityViterboItaly

Personalised recommendations