A series of fused isoxazolidines have been prepared via 1,3-dipolar cycloaddition reactions of N-protected methylenenitrones with 1,3-dimethyluracil derivatives, and their NMR spectra have been recorded in TFA-d and in CDCl3 over a wide range of temperatures. The spectra indicate the presence of two invertomers for all isoxazolidines. Barriers to nitrogen inversion in the cycloadduct 6a have been determined using DFT quantochemical calculations. Our estimates have shown that the inversion proceeds at more complex path, involving four structures of local minima and four transition states.
A number of nucleosides, nucleotides and nucleic acid bases with modified pyrimidine moieties are currently undergoing evaluation as antiviral agents and modifications primarily at the 5- and/or 6-position of the pyrimidine base have been extensively studied . Part of this effort has involved the utilization of the C5=C6 double bond for synthetic elaboration by means of nucleophilic and electrophilic reactions  or cycloaddition processes. Although the intermolecular cycloaddition reaction is one of the most versatile tools for the synthesis of fused heterocyclic systems [3–5], only a few examples of their application to the modification of uridine and uracil derivatives are reported in the literature. Keana et al.  described the preparation of the key intermediate for tetrodotoxin derivative in the Diels–Alder reaction of butadiene with appropriately functionalized orotates. Saladino et al.  reported the reaction of lithium trimethylsilyldiazomethane and diazomethane with uracil. Pellissier et al.  investigated the cobalt-mediated [2.2.2] cycloaddition of pyrimidine derivatives to alkynes, and Negron and coworkers obtained bicyclic N-methylpyrrolidine thymidine derivatives using cycloaddition reaction of azomethine ylide with bis-O-silylthymidines . Finally, Colacino and coworkers obtained in reaction between nitrone and 1-N-vinyluracil a new isoxazolidine derivative fused with the pyrimidine moiety, as a competitive product to 4′-aza-analogues of 2′,3′-dideoxynucleotides .
Nitrones are valuable synthons in the organic synthesis which are extensively used in the synthesis of biologically active compounds [11, 12], as spin trap reagents , as therapeutic agents [14, 15] and primarily behave like 1,3-dipoles in cycloaddition reactions giving isoxazolidines . Isoxazolidines are already well established as heterocyclic analogues of the five-membered sugar moiety of nucleosides, and a variety of isoxazolidine-based molecules are being tested for antiviral and anticancer activities .
The 1,3-dipolar cycloaddition of the nitrone dipole to the double bond provides a convenient entry into the isoxazolidine ring system having an N–O moiety embedded in the ring skeleton. Generally, it takes place in a regioselective and stereocontrolled manner, because the cycloaddition reaction conserves the stereochemistry of the alkene. However, the conformational aspect is not so obvious and the conformation of very flexible five-membered ring is not well defined . Moreover, it has been shown that unshared orbital interactions on neighboring heteroatoms such as those present in isoxazolidines are responsible for a considerable energy barrier toward ring inversion . Oxygen being next to nitrogen raises the barrier to nitrogen inversion to such an extent that the individual invertomers can be identified by NMR spectroscopy [20, 21].
N-Cyclohexylhydroxylamine , 5-chlorouracil , 1,3-dimethy-5-nitrouracil  and 1,3-dimethyl derivatives of uracils 2, 3 and 5 were prepared according to the literature . All other starting materials and reagents were obtained from Sigma-Aldrich. Toluene was distilled from NaH and was stored over molecular sieves (0.4 nm). All reactions were monitored by TLC. Thin-layer chromatography was performed with 60 F254 TLC plates (Merck). Column chromatography was performed with silica gel (Merck, particle size 0.063–0.200 mm, 70–230 mesh). The cycloaddition reactions were carried out in glass pressure tubes equipped with a magnetic stirrer.
1H NMR, 13C NMR and 19F NMR spectra were performed on Varian GEMINI 300 (300 MHz), Varian 400 (400 MHz), Bruker Avance 400 (400 MHz) and Bruker Avance 600 (600 MHz) spectrometers. Temperature control was achieved using a Bruker cooling unit (BCU-II) to provide chilled air. The experiments were performed without sample spinning. Chemical shifts of 1H NMR were expressed in parts per million downfield from tetramethylsilane (TMS) as an internal standard (δ = 0) in CDCl3. Chemical shifts of 13C NMR were expressed in parts per million downfield from CDCl3 as an internal standard (δ = 77.0). Chemical shifts of 19F NMR were expressed in parts per million upfield from CFCl3 as an internal standard (δ = 0) in CDCl3.
The mass spectra were recorded on a 320MS/450GC Bruker and AMD 402 spectrometer, and ionization was achieved through electron impact (EI). High-resolution data were obtained on the same instrument using a peak-matching technique. Elemental composition of the discussed ion was determined with an error of less than 10 ppm in relation to perfluorokerosene at resolving power of 10000. The elemental analyses were made on Vario EL III Element Analyzer.
All computations in this study including DFT NMR calculations were performed by Gaussian 09 program package . The DFT geometry optimizations have been performed using different functional, namely B3LYP , WB97XD , M06  with 6–31G(d) and 6–31++G(d,p). Geometry optimizations were confirmed by the vibrational frequency calculations at each level. In DFT NMR calculations, the geometry optimizations were calculated using the B3LYP method on 6–31G(d), 6–31G(d,p), 6–31++G(d,p), 6–31G(2d,2p) and 6–31++G(2d,2p) level of theory. Solution-state calculations used default and IEFPCM  model, as implemented in Gaussian 09. 13C NMR chemical shifts were computed at the B3LYP/6–31G(d), 6–31G(d,p), 6–31++G(d,p), 6–31G(2d,2p) and 6–31++G(2d,2p) level using the GIAO method  and are given relative to that of TMS calculated at the same level of theory.
The quantochemical research was supported in part by PL-Grid Infrastructure.
Typical synthetic procedure
A mixture of paraformaldehyde (60 mg, 2 mmol), triethylamine (222 mg, 2.2 mmol) and N-methylhydroxylamine hydrochloride, N-t-butylhydroxylamine or N-benzylhydroxylamine hydrochloride (2 mmol) in anhydrous toluene (10–15 mL) was placed in a glass pressure tube equipped with a magnetic stirrer and stirred at 70 °C for 1 h. Only N-cyclohexylhydroxylamine was used as a free base. The solution was cooled to room temperature, the corresponding 1,3-dimethyluracils 2–5 (1 mmol) was then added, and the solution was heated at 60–80 °C for 24–48 h. The solvent was evaporated to dryness under reduced pressure, and 10–15 mL of water was added. The aqueous solution was extracted a few times with CHCl3 and the combined extract dried over Na2SO4. Solvent was removed, and the crude product was separated by column chromatography (silica gel, hexane, a gradient of hexane/CH2Cl2, CH2Cl2 or a gradient of hexane/ethyl acetate) to give compounds 6a–9d. The detailed spectral data of compounds 6a–9d and 10a–10d can be found in the Supporting Information, and the representative analytical data of compound 6a are given below.
Analytical data of compound 6a
Compound 6a 3a-Fluoro-2,5,7-trimethyltetrahydroisoxazolo[5,4-d]pyrimidine-4,6(2H,5H)-dione
Oil, 78 % yield. Room temperature NMR: 1H NMR (300 MHz, CDCl3) δ 5.18 (d, J = 18.3 Hz, 1 H, C6–H), 4.00–3.20 (vbs, 2 H, CH2), 3.17 (bs, 3 H, N-CH3), 3.00 (bs, 3 H, N–CH3), 2.71 (bs, 3 H, N–CH3). 19F NMR (282 MHz, CDCl3) δ −159.06 (bs), −159.77 (bs). TFA NMR: 1H NMR (400 MHz, TFA-d) Major invertomer: δ 6.08 (d, J = 11.7 Hz, 1 H, C6–H), 5.24 (dd, J = 13.8, 28.9 Hz, 1 H, C9–Ha), 4.46 (dd, J = 13.8, 21.9 Hz, 1H, C9–Hb), 3.69 (s, 3 H, N-CH3), 3.40 (s, 3 H, N–CH3), 3.23 (s, 3 H, N–CH3). Minor invertomer: δ 6.15 (d, J = 14.3 Hz, 1 H, C6–H), 5.23 (m, 1 H, C9–Ha), 4.46 (dd, J = 13.6, 27.1 Hz, 1 H, C9–Hb), 3.67 (s, 3 H, N–CH3), 3.43 (s, 3 H, N–CH3), 3.32 (s, 3 H, N–CH3). Low-temperature NMR: 1H NMR (600 MHz, CDCl3) Major invertomer: δ 5.23 (d, J = 17.8 Hz, 1 H, C6–H), 3.70 (t, J = 9.9 Hz, 1 H, C9–Ha), 3.05 (dd, J = 10.6, 24.9 Hz, 1 H, C9–Hb), 3.18 (s, 3 H, N–CH3), 2.99 (s, 3 H, N–CH3, 2.74 (s, 3H, N–CH3). Minor invertomer: δ 5.16 (d, J = 19.6 Hz, 1 H, C6–H), 3.83 (dd, J = 12.5, 20.6 Hz, 1 H, C9–Ha), 2.78 (dd, J = 12.5, 27.8 Hz, 1 H, C9–Hb), 3.78 (s, 3 H, N–CH3), 3.18 (s, 3 H, N–CH3), 2.71 (s, 3 H, N–CH3). 19F NMR (565 MHz, CDCl3) δ −159.71 (m), −158.93 (m). 13C NMR (151 MHz, CDCl3) Major invertomer: δ 165.40 (d, J = 26.4 Hz), 150.56, 97.12 (d, J = 193.4 Hz), 88.45 (d, J = 32.8 Hz), 67.80 (d, J = 28.2 Hz), 44.49, 32.44, 28.59. Minor invertomer: δ 164.79 (d, J = 26.4 Hz), 150.20, 95.70 (d, J = 195.4 Hz), 88.76 (d, J = 35.2 Hz), 67.14 (d, J = 25.6 Hz), 44.87, 34.75, 28.40. MS (EI, %) m/z 217 (M+, 13 %). HRMS (EI) calcd for C8H12FN3O3: 217.08627, found: 217.08503. Elemental analysis. Found: C, 44.12; H, 5.73; N, 19.11. Calc. for C8H12FN3O3 (217.20): C, 44.24; H, 5.57; N, 19.35 %.
Results and discussion
In the course of our work on fluorovinyl derivatives of nucleic acid bases and their cycloaddition reactions with nitrones [32–34], we decided to synthesize a series of isoxazolidinyl derivatives of 5-fluorouracil in 1,3-dipolar cycloaddition of N-fluorovinyl-5-fluorouracil with nitrones. Unexpectedly, we obtained exclusively, without any competitive reaction, the cycloaddition product not to exocyclic, but to endocyclic double bond with the formation of fused ring. To our knowledge, there was only one report that deals with the reaction of pyrimidines with nitrones . In continuation of our recent studies, we decided to apply the nitrone–olefin cycloaddition to the formation of fused isoxazolidines in reactions of methylenenitrones with 5-substituted 1,3-dimethyluracils, to evaluate the reaction regioselectivity and the conformation/configuration of the resultant cycloadducts. Thus, condensation reaction of N-alkyl hydroxylamines with formaldehyde followed by in situ 1,3-dipolar cyclization of the resulting achiral nitrones with pyrimidinic nucleophiles affords compounds 6a–6d, 7a–7d, 8a–8d, 9a and 9d (Scheme 1). The obtained fused isoxazolidines showed spectral properties that are typical for systems exhibiting slow inversion on the nitrogen atom—a significant broadening of signals in the spectra of proton and carbon NMR. They represent the additional examples of molecules which can exhibit chemical shift nonequivalence due to hindered stereomutation at nitrogen [35–37].
The cycloaddition took place with complete regioselectivity and with stereospecific generation of two consecutive stereocenters. The theoretical studies have indicated that 1,3-dipolar cycloaddition reactions proceed through a concerted mechanism , and therefore, we only considered the one step mechanism for the 1,3-dipolar cycloaddition reaction of nitrone–uracil. Indeed, cycloaddition led to products with the cis ring junction stereochemistry what was deduced by the coupling constant values in 1H NMR spectra between newly formed ring juncture atoms in compounds 6a–6d and 7a–7d. Since nitrones 1a–1d are achiral, the obtained isoxazolidines differ only in the configuration of C5 and C6 carbon atoms, and thus, obtained [4.3.0] bicyclic systems have two chiral centers which give indistinguishable pair of enantiomers. The regioselectivity of cycloaddition reaction results from the structure of the uracil moiety. Carbon atoms C2, C4 and C6 in pyrimidine ring are characterized with diminished electron density in relation to carbon atom C5 . Uracil exhibits a comparable to pyrimidine electron density; however, electron density at C5 atom is additionally increased by conjugation of π electrons with electrons of oxygen atom . The electron density distribution in the uracil ring reflects nucleophilic and electrophilic properties of the double bond in uracils and the regioselective process of the cycloaddition. No other isomers were observed in the reaction mixtures.
However, the N-substituted isoxazolidinyl ring fused with almost planar six-membered ring may exhibit a special type of isomerism found in compounds with a substituent on a bridged ring system—isomerism endo and exo. The environment of the N-alkyl group in two isomers differs considerably. In exo isomer the bridgehead substituent R 1 is anti to the nitrogen lone pair of electrons, while in endo it is syn to the lone pair of electrons (Fig. 1). A third center of symmetry associated with chirality of the nitrogen atom appears, and the two diastereoisomers, under specific conditions, should be observable.
Compounds 6a–9d gave broad 1H and 13C NMR signals at room temperature. This is particularly distinct for the CH2 protons of the isoxazolidinyl ring in 1H NMR spectra. In compounds 6 and 7, the geminal protons of CH2 group adjacent to the asymmetric carbon atom are diastereotopic and should give in 1H NMR a clear AB doublets of doublets. The broadening is a result of a dynamic equilibrium between the two diastereoisomers with a different configuration for the asymmetric nitrogen atom, and the rapid inversion at room temperature of conformationally labile chiral center simplifies the AB system to A2 system. Thus, the 1H NMR spectra of compounds 6a–6d which have a fluorine atom in the C5 showed at 5.08–5.17 ppm a doublet (J = 18 Hz) ascribed to the C6–H proton, very broadened signals of CH2 group in the range of 2.90–3.70 ppm, two distinguishable singlets from N 1- and N 3-methyl groups and signals assigned to substituents on the isoxazolidinyl nitrogen atom. 19F NMR spectra showed at −159.20 to −159.40 ppm a broad singlet deriving from the C5–F. However surprisingly, for compound 5a we observed two broad signals of fluorine atom at −159.06 and −159.77 ppm, respectively.
Very similar 1H NMR spectra were observed for compounds 7a–7d and 8a–8d (with the exception of compounds 7a and 8a). For 7a–7d proton, C6-H appears at 5.11–5.21 ppm as a doublet coupled to C5–H (J = 7.5–8.0 Hz). The same coupling constants are also observable for C5–H which appears at 3.55–3.60 ppm as a multiplet. Respectively, protons C6–H for 8b–8d appear at 5.35–5.39 ppm as a broad singlet. Geminal methylene protons of the isoxazolidinyl ring are indistinguishable and appear as a broad signal. Only 1H NMR spectra of isoxazolidines 7a and 8a having N-methyl substituent in the five-membered ring were somewhat unexpected. In compound 7a, two diastereotopic methylene protons were visible, respectively, at 3.66 and 2.90 ppm. In compound 8a, two sets of diastereotopic protons, broad but very distinguishable at 4.57, 3.97, 3.73 and 3.08 ppm and two diastereoisomeric protons C6–H at 5.27 and 5.48 ppm were observed.
We studied the temperature dependence of the NMR spectra of 6a–6d and 7a–7d series in CDCl3 within the temperature range from 273 to 223 K. Unfortunately, compounds 8a–8d were chemically unstable and were not taken into account in the temperature study. On lowering the temperature (up to 223 K), the broad spectral lines become sharper and show two distinct forms of the compounds. The rate of nitrogen atom inversion at a sufficiently low temperature is decreased so much that signals of two invertomers are observed in the 1H, 19F and 13C NMR spectra of examined compounds. These invertomers have different configuration at the stereogenic nitrogen atom, and we assigned them to two forms—exo and endo. The two most likely configurations of the invertomers of the isoxazolidines fused with six-membered ring are shown in Fig. 1. The figure ignores completely any conformational aspect of five-membered ring (flipping of the envelope or the twisted form). In similar systems, it is assumed that the preference of the N-substituent for a less hindered environment is greater than that of the lone pair . We suppose that we well assigned the more intense signal to the exo isomer where the lone pair of electrons of the nitrogen atom is in the energetically favorable anti orientation to substituents at bridgehead carbon atoms. This also agrees with our calculations performed on compound 6a.
We have also made 1H NMR spectra of all compounds 6–8 and additionally 9a and 9d in TFA-d to afford for 6a–6d, 8a–8d, 9a and 9d protonated diastereoisomeric pairs of invertomers. The protonation of the lone electron pair of nitrogen in amines effectively inhibits the process of inversion. Protonation on the nitrogen atom of isoxazolidinyl ring “freezes” its configuration and eliminates the possibility of moving the configurational form one to another, and what is important, it also “freezes” the existing at a given temperature invertomers at their thermodynamic equilibrium. Thus, isoxazolidines were dissolved in TFA-d and 1H NMR spectra were recorded immediately. Protonation of compounds 6, 8, 9 and a solvent effect lead generally to downfield shifts of all signals in the 1H NMR spectra. Each time, except for the spectra of compounds 7a–7d, two sets of signals were obtained for all protons corresponding to the two diastereomeric forms of compounds. Only isoxazolidinyl derivatives 7a–7d were very susceptible to acid, and in TFA-d, they immediately underwent the process of acid-mediated N–O cleavage to furnish quantitatively N-alkylated-C5-methylamino derivatives of barbituric acid 10a–10d (Scheme 2). Interestingly, 1H NMR spectra of 10a–10d showed two nonequivalent geminal Hc-C-Hd protons (J = 13.6–13.9 Hz), which suggests the existence of strong intramolecular hydrogen bond and formation of the pseudo six-membered ring. This is somewhat unexpected because trifluoroacetic is a very effective hydrogen bond breaker. Moreover, the 1H NMR spectra performed in CDCl3, and in CDCl3 after addition of D2O, showed exactly the same pattern.
The ratios of two invertomers for compounds 6a–6d and 7a–7d together with experimental temperatures are shown in Table 1. Taking into account the uncertainty in determining the coalescence temperature, we gave the temperature at which well-developed signals of two diastereomers are clearly visible. Also the ratios of stereoisomeric quaternary isoxazolidines 6a–9d are shown in Table 1.
Chemical shift values for protons in the isoxazolidinyl ring for diastereoisomers 6a–9d are given in Table 2. In temperature experiments, the 1H NMR analysis for the minor isomers is incomplete in some causes due to peak overlap. Spectra recorded in TFA-d gave a distinct separation of two diastereoisomeric forms.
At room temperature, all signals on the 1H NMR and 19F NMR and 13C NMR spectra were broadened as a result of the participation of the compounds 6a–6d and 7a–7d in the relatively slow process of a pyramidal nitrogen inversion. To study the nature of dynamic processes, a series of variable temperature NMR experiments in range of 298–223 K were carried out. As was mentioned, the low-temperature 1H, 19F and 13C NMR spectra of 6a–6d and 7a–7d show separated signals for the two invertomers. The temperature at which the spectrum reaches this state (coalescence temperature) is mostly dependent on the substituent on the isoxazolidinyl nitrogen atom.
Compounds 6a–6c and 7a–7c achieve very distinct separation of two invertomers in 1H NMR (and 19F NMR for compounds 6) at 243 K (Fig. 2) and at temperatures somewhat higher, while compounds 6d and 7d having t-butyl substituent achieve this state until at 223 K (Fig. 3).
What is more, for the compound 6a with the smallest alkyl substituent on the isoxazolidinyl nitrogen atom, the initial separation of signals for two invertomers is visible at 263 K and for compound 7a already at 273 K (Fig. 4).
This is in good accord with the previous literature reports. Effects of substituents on the inversion rates of cyclic amines  and isoxazolidines [20, 35] have been studied by the NMR method, and it has been found that bulky groups either on the ring or on the nitrogen tend to increase the inversion rates, presumably by destabilizing the separate nonplanar configurations relative to the transition state. The bulky t-Bu group in sp3-hybridized nitrogen increases the ground-state energy, and the sp2-hybridized transition state, through which the nitrogen inversion occurs, has a much smaller steric congestion; hence, the activation barrier is lower than that of methyl-substituted nitrogen.
The notable conformational feature of the 1H NMR spectra of obtained invertomers, both performed in TFA-d and at low temperatures in CDCl3, is in most cases a large chemical shift anisotropy for germinal protons of isoxazolidinyl methylenes (Δδ = 0.21–1.00 ppm for major invertomer and Δδ = 0.28–1.13 ppm for minor invertomer). The significant differences for chemical shifts for germinal protons can be explained by considering the isoxazolidine ring geometry, it adopts the lowest energy conformation, an envelope conformation, and allowing for inversion, its nitrogen atom will either extend out from the envelope or point inside the envelope.
Only for minor invertomers of 8a–8d, very small values of chemical shift anisotropy were observed. The Δδ values for 8a are admittedly 0.28 ppm, but for 8b Δδ is only 0.09 ppm. For compounds 8c and 8d, the anisotropy disappears entirely and the two doublets in the typical AB system go into a broad singlet. The major invertomers of 8a–8d show Δδ values in the range from 0.47 to 1.00 ppm. Generally, the considerable Δδ values of chemical shifts for isoxazolidinyl germinal protons in all the investigated compounds seem to be more dependent on isoxazolidinyl N-alkyls than from the C5-substituents, and in all cases, they show the biggest value for methyl group and smallest value for t-butyl substituent.
To verify it, we synthesized compounds 9a and 9d having more abundant chlorine atom in the C5 position, to compare them with similar 6a–8a and 6d–8d. Unfortunately, 1H NMR spectra performed in TFA-d did not confirm fully our expectations; only for the minor invertomer the mentioned above relationship was maintained, and for major invertomer of 9a a very small value of chemical shift anisotropy was observed. Thus, the establishment of correlation of the anisotropic effect in the tested compounds with the size of the N-alkyl substituent can be sometimes a small overinterpretation, although this factor is crucial, and it mainly has an effect on the slight conformational changes in the five-membered ring.
The inspection of the 1H NMR data reveals that the geminal coupling constants of the minor invertomer in TFA-d are without any exception smaller than those in the dominant invertomer. Simultaneously, for compounds 6a–6d the coupling constants (if visible) of methylene protons with vicinal fluorine C5-F in dominant invertomer are smaller than those in the minor invertomer. Conceivably, this generality may help to determine the distinguishability of two invertomers with a different configuration for the asymmetric nitrogen atom in more complicated systems. For C6-H protons, two separate sets of signals appear in temperature spectra in the range 5.04–5.27 ppm and in the range 6.02–6.52 in spectra performed in TFA-d as sharp doublets for compounds 6 and 7 (J HH = 6–8 Hz, J HF = 22–29 Hz) and as singlets for compounds 8 and 9. For compounds 6c, 7c and 8c having a benzyl substituent, in the 1H NMR spectra two signals of diastereotopic benzyl protons are also observable. Two sets of two doublets in the temperature spectra appear in the range 3.99–4.03 ppm (J = 13.6–13.8 Hz) and in spectra performed in TFA-d in the range 4.90–5.02 ppm (J = 13.4–14.0 Hz). This is a strong evidence for the existence of “frozen” stereogenic center, adjacent to the CH2 group.
And finally, it is also noteworthy that the nature of the N-substituents has little effect on the ratio between the two diastereoisomers of isoxazolidines. The exception is the value obtained for compound 7a which is different from the pattern of the population trends in these systems, and it means the more abundant substituent, the higher ratio (see Table 1). Additionally for compounds 6a–6d, there is a very good agreement between values obtained in the low-temperature spectra as well as in the TFA-d spectra. The differences in the ratios of two invertomers result from different thermodynamic equilibriums in different temperatures.
NMR analysis at low temperatures has proven the presence of slow nitrogen inversion process. For a clear rationalization of observed results, DFT quantochemical calculations were performed to optimalize the most likely geometries of compound 6a individual isomers and to designate the energy barrier of the observed inversion.
Preliminary geometry optimizations with B3LYP method and 6-31G(d) basis set allowed to optimize two structures differing by the nitrogen atom configuration and isoxazolidine ring conformation. In order to calculate the energy barrier of inversion between those two structures, it was necessary to find a TS structure between both minima (Scheme 3). Our research has shown that such structure probably does not exist, and the inversion proceeds at more complex path, involving additional two structures of local minima (Fig. 5) and four transition states.
In contrast to saturated six-membered rings which usually possess well-determined conformations, five-membered rings are much more flexible, so the conformation is difficult to ascertain . The isoxazolidines having five-member ring system can exist in several puckered conformations with the geometry of envelope and half-chair. Ring puckering would lead to the most stable conformation of molecule which generally occurs in placing the substituents in the favorable pseudoequatorial orientation . Furthermore, it has been shown that gauche relationship between the lone pairs of nitrogen and oxygen atoms is also the stabilizing factor of the whole structure . The geometry optimized structures of molecule 6a (Fig. 5) represent the group of geometries that fulfill those rules. Structures 6a–a and 6a–b have minimum total energy, and methyl substituent on the nitrogen atom is placed in pseudoequatorial orientation. Furthermore, in structures 6a–c and 6a–d methyl group is localized pseudoaxially with gauche orientation of the nitrogen and oxygen lone pairs. By placing all four structures on the potential energy surface, geometries 6a–a and 6a–b have the lowest total energy which could show that in our model the steric effects have the greatest impact on the most stable structure. Moreover, the structure 6a–d has the highest total energy of all optimized minima which seems to confirm this rule. According to calculated total energies, molecule 6a is expected to exist in two forms 6a–a and 6a–b, which differ from each other by both nitrogen configuration and five-membered ring conformation. It becomes clear that to predict the theoretical energy of inversion between those minima, calculations should comply not only nitrogen configuration change, but also ring flip of the isoxazolidine.
We assume that in our model the inversion process begins with low-energy pseudorotation change of structure 6a–a into the conformer 6a–c. Relatively slow nitrogen inversion would next transform 6a–c into the isomer 6a–b. Pseudorotation of 6a–b into 6a–d followed by nitrogen inversion completes the dynamic cycle of the observed process (Fig. 6).
The B3LYP/6-31G(d) geometry optimized structures were used as starting points for other calculations. For brevity, the 6-31 ++G(d,p) basis set was chosen for the DFT (B3LYP, M06 and WB97XD) geometry optimizations of all minima and potential TS structures. The comparison of calculated total (H) and relative (ΔE, kcal/mol) energies is shown in Tables 3 and 4.
Experimental NMR spectra measured in CDCl3 at low temperatures and TFA-d do not provide the direct evidence which isomer (6a–a or 6a–b) is preferred. However, both the 1H and 13C chemical shifts show some significant differences for the major and minor forms. The computational chemical shift calculations were performed to find some relations of these differences to the structural changes. In order to find the most suitable computational model, calculations were carried out using DFT (B3LYP) method with different basis sets: 6–31G(d), 6–31G(d,p), 6–31++G(d,p), 6–31G(2d,2p) and 6–31++G(2d,2p). All the significant relative changes in 13C NMR chemical shifts were reproduced, and DFT GIAO calculations allowed to assign the major form of compound 6a to the 6a–a configuration and the minor form of compound 6a to the 6a–b configuration (Table 6).
Linear correlation between the chemical shift values of 13C NMR measured experimentally and calculated using B3LYP/6–31++G(2d,2p) demonstrates a satisfactory assignment of the experimental chemical shift values to the calculated structures of 6a. An R 2 = 0.9988 of major to 6a–a structure and R 2 = 0.9992 of minor to 6a–b structure indicate that the regression line perfectly fits the data. Moreover, GIAO B3LYP/6–31++G(2d,2p)/IEFPCM(CHCl3) calculations between relative chemical shifts of 6a–a and 6a–b are linearly correlated (R 2 = 0,9667) with those observed experimentally (Fig. 7).
In general, our theoretical investigation of compound 6a has shown that nitrogen-hindered configuration change in fused isoxazolidines is not a simple one step process. Moreover, the proper inversion of nitrogen atom is possible only after reaching one of the molecules geometry that has this same configuration of nitrogen but the opposite conformation of the ring compared to starting geometry. According to the B3LYP calculations performed with 6–31++G(d,p) basis set, the energy barrier for the nitrogen inversion as a total 6a–a to 6a–b inversion process is approximately 13.5 kcal/mol. The value of 13.2–13.4 kcal/mol has been reported for similar fused isoxazolidinyl systems . DFT GIAO calculations demonstrate compliance of experimental and calculated chemical shift values for major and minor invertomer of compound 6a.
In this study, we have synthesized fused isoxazolidines via an intramolecular 1,3-dipolar cycloaddition of various N-alkyl methylenenitrones with 5-substituted uracil derivatives. The cycloadducts were characterized by spectroscopic and structural techniques as well as the theoretical methods. These reactions proceed with complete diastereoselectivity and regioselectivity. The obtained isoxazolidines exist as equilibrating mixtures of invertomers due to slow nitrogen inversion. The NMR studies on the conformation of invertomers and on the inversion rates showed the effect of N-alkyl substituents and C5-substituents both on the geometry of the ring and on the inversion barrier. The nitrogen inversion barrier for 6a was determined using DFT quantochemical calculations and was found to be 13,5 kcal/mol. The experimental and theoretical 13C NMR chemical shifts are in good agreement with each other. In addition, the theoretical relative chemical shifts of 6a–a and 6a–b showed very good correlation with the experimental data. Further theoretical investigation on nitrogen inversion of the title compounds is currently under way.
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Kuprianowicz, M., Kaźmierczak, M. & Wójtowicz-Rajchel, H. The nitrogen inversion in fused isoxazolidinyl derivatives of substituted uracil: synthesis, NMR and computational analysis. Struct Chem 27, 1265–1278 (2016). https://doi.org/10.1007/s11224-016-0755-4
- Nitrogen inversion
- Quantum chemical calculations
- NMR spectroscopy