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DNA interaction with synthetic polymers in solution

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Abstract

DNA complexes with cationic polymers (polyvinylamine (PVA), polyallylamine (PAA), polydimethylaminoethylmethacrylate (PDMAEM), poly-(N,N,N-trimethylammonio)ethyl methacrylate chloride (PTMAEM), poly-l-lysine (PLL)) were investigated. It was shown that volume and persistent length of DNA do not change essentially at low cationic polymer concentration in a solution. DNA packaging in 0.005 M NaCl was observed at charge ratio N/P ≈ 1. Secondary DNA structure in complexes was not disrupted, and DNA was protected from protonation. The comparison between DNA packaging in complexes with polycations and DNA condensation induced by trivalent ions was made.

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Correspondence to Nina Kasyanenko.

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Kasyanenko, N., Afanasieva, D., Dribinsky, B. et al. DNA interaction with synthetic polymers in solution. Struct Chem 18, 519–525 (2007). https://doi.org/10.1007/s11224-007-9162-1

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Keywords

  • DNA-polymeric complexes
  • DNA condensation
  • Polyvinylamine
  • Polyallylamine
  • Polydimethylaminoethylmethacrylate
  • Polytrimethylammonioethyl methacrylate
  • Poly-l-lysine