Russian Journal of Genetics

, Volume 41, Issue 12, pp 1434–1436 | Cite as

Sequence and Structure Analysis of Cryptic Plasmid pN30 from Oil-Oxidizing Strain Rhodococcus erythropolis 30

  • L. E. Ryabchenko
  • A. D. Novikov
  • P. N. Golyshin
  • A. S. Yanenko
Short Communications


Nucleotide sequence of cryptic plasmid pN30 from a Rhodococcus erythropolis 30 soil isolate was determined. Plasmid DNA consists of 5403 nucleotide pairs and contains about 62% GC pairs, which is typical of Rhodococcus DNA. No significant homology was determined between the pN30 DNA sequence and those of known plasmids. Computer-aided analysis of pN30 sequence revealed open reading frames that encode proteins strongly homologous to replicative proteins encoded by small cryptic plasmids of different actinomycetes.


Nucleotide Nucleotide Sequence Structure Analysis Significant Homology Replicative Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Larkin, M., De Mot, R., Kulakov, L., and Nagy, I., Applied Aspects of Genetics, Antonie van Leeuwenhoek, 1998, vol. 74, pp. 133–153.CrossRefPubMedGoogle Scholar
  2. 2.
    Finnerty, W.R., The Biology and Genetics of the Genus Rhodococcus, Ann. Rev. Microbiol., 1992, vol. 46, pp. 193–213.Google Scholar
  3. 3.
    Sambrook, J., Fritsch, E., and Maniatis, T., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1989.Google Scholar
  4. 4.
    Birnboin, H.C. and Doly, J., A Rapid Alkaline Extraction Procedure for Screening Recombinant Plasmid DNA, Nucleic Acids Res., 1979, vol. 7, pp. 1513–1523.Google Scholar
  5. 5.
    Vogt Singer, M.E. and Finnerty, W.R., Construction of an Escherichia coli-Rhodococcus Shuttle Vector and Plasmid Transformation of Rhodococcus spp., J. Bacteriol., 1988, vol. 170, pp. 638–645.Google Scholar
  6. 6.
    Goodfellow, M., Alderson, G., and Chun, I., Rhodococcal Systematics: Problems and Developments, Antonie van Leeuwenhoek, 1998, vol. 74, pp. 3–20.CrossRefPubMedGoogle Scholar
  7. 7.
    De Mot, R., Nagy, I., De Schrijver, A., et al., Structural Analysis of the 6-kb Cryptic Plasmid pFAJ2600 from Rhodococcus erythropolis NI86/21 and Construction of Escherichia coli-Rhodococcus Shuttle Vectors, Microbiology, 1997, vol. 143, pp. 3137–31.PubMedGoogle Scholar
  8. 8.
    Kulakov, L.A., Larkin, M.J., and Kulakova, A.N., Cryptic plasmid pKA22 Isolated from the Naphthalene-Degrading Derivative of Rhodococcus rhodochrous NCIMB13064, Plasmid, 1997, vol. 38, pp. 61–69.CrossRefPubMedGoogle Scholar
  9. 9.
    Hashimoto, J., Nishiyama, M., Yu, I., et al., Development of a Host Vector System in a Rhodococcus Strain and Its Use for Expression of the Cloned Nitrile Hydratase Gene Cluster, J. Gen. Microbiol., 1992, vol. 138, pp. 1003–1010.PubMedGoogle Scholar
  10. 10.
    Rauzier, J., Moniz-Pereira, J., and Gicquel-Sanzey, B., Complete Nucleotide Sequence of pAL5000, a Plasmid from Mycobacterium fortuitum, Gene, 1988, vol. 71, no.2, pp. 315–321.CrossRefPubMedGoogle Scholar

Copyright information

© MAIK "Nauka/Interperiodica" 2005

Authors and Affiliations

  • L. E. Ryabchenko
    • 1
  • A. D. Novikov
    • 1
  • P. N. Golyshin
    • 2
  • A. S. Yanenko
    • 1
  1. 1.State Research Institute of Genetics and Selection of Industrial MicroorganismsMoscowRussia
  2. 2.German Research Centre for BiotechnologyBraunschweigGermany

Personalised recommendations