Russian Journal of Genetics

, Volume 41, Issue 12, pp 1372–1380 | Cite as

Identification of LMW Glutenin-Like Genes from Secale sylvestre Host

  • H.-Y. Shang
  • Y.-M. Wei
  • H. Long
  • Z.-H. Yan
  • Y.-L. Zheng
Plant Genetics


Three low-molecular-weight (LMW) glutenin-like genes (designated as Ssy1, Ssy2, and Ssy3) from Secale sylvestre Host were isolated and characterized. The three genes consist of a predicted highly conservative signal peptide with 20 amino acids, a short N-terminal region with 13 amino acids, a highly variable repetitive domain and a less variable C-terminal domain. The deduced amino acid sequences of the three genes were the LMW-m type due to a methionine residue at the N-terminus. The phylogenetic analysis indicated that the prolamin genes could be perfectly clustered into five groups, including HMW-GS, LMW-GS, α/β-, γ-, and κ-prolamin. The LMW glutenin-like genes of S. sylvestre were more orthologous with the LMW-GS genes of wheat and B hordein genes of barley, which also had been confirmed by the homology analysis with the LMW-GS of wheat at Glu-A3, Glu-B3, and Glu-D3 loci. These results indicated that a chromosome locus (designated as Glu-R3) might be located on the R genome of S. sylvestre with the functions similar to the Glu-3 locus in wheat and its related species.


Peptide Methionine Signal Peptide Deduce Amino Acid Sequence Chromosome Locus 
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Copyright information

© MAIK "Nauka/Interperiodica" 2005

Authors and Affiliations

  • H.-Y. Shang
    • 1
  • Y.-M. Wei
    • 1
  • H. Long
    • 1
  • Z.-H. Yan
    • 1
  • Y.-L. Zheng
    • 1
  1. 1.Triticeae Research InstituteSichuan Agricultural UniversityDujiangyan, SichuanChina

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