Russian Chemical Bulletin

, Volume 68, Issue 1, pp 174–180 | Cite as

l-Arginine and nitric oxide synthesis in the cells with inducible NO synthase

  • Z. V. KuroptevaEmail author
  • L. M. Baider
  • L. G. Nagler
  • T. N. BogatyrenkoEmail author
  • O. L. BelaiaEmail author
Full Articles


The effect of citrulline and ammonium chloride on the nitric oxide formation by peritoneal macrophages and liver tissue cells was studied using ESR spectroscopy. In ex vivo models, the incubation of cells capable of expressing inducible NO synthase (iNOS) with interferon-γ resulted in a moderate increase in the amount of hemoglobin–nitric oxide nitrosyl complexes (Heme–NO NCs), whereas incubation with l-citrulline and ammonium chloride increased the amount of Heme–NO NCs by an order of magnitude. It was assumed that a separate cycle of L-arginine and nitric oxide synthesis exists in the peritoneal macrophages and liver cells, with the major participants of the cycle being the inducible NO synthase enzyme (iNOS) and enzymes that synthesize L-arginine from L-citrulline and a nitrogen source. Functioning of this cycle makes immunocompetent cells with iNOS able to produce NO for a long time and in large amounts.

Key words

nitric oxide arginine and nitric oxide cycle liver cells peritoneal macrophages inducible NO synthase citrulline ammonium chloride ESR spectroscopy 


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© Springer Science+Business Media, Inc. 2019

Authors and Affiliations

  1. 1.N. M. Emanuel′ Institute of Biochemical PhysicsRussian Academy of SciencesMoscowRussian Federation
  2. 2.Institute of Problems of Chemical PhysicsRussian Academy of SciencesChernogolovkaRussian Federation
  3. 3.Moscow State University of Medicine and DentistryMoscowRussian Federation

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