Advertisement

Russian Chemical Bulletin

, Volume 67, Issue 11, pp 1997–2002 | Cite as

Effect of the type and concentration of mobile phase additives on the separation of salbutamol sulfate enantiomers in supercritical fluid chromatography

  • M. O. Kostenko
  • O. I. Pokrovskiy
  • O. O. Parenago
  • V. V. Lunin
Full Articles
  • 10 Downloads

Abstract

The influence of a set of additives of various nature on the separation of salbutamol sulfate enantiomers by supercritical fluid chromatography on the stationary phase based on amylose tris(3-chloro-5-methylphenylcarbamate) is considered. The application of water and acidic additives does not lead to enantioseparation. The use of hexafluoroisopropyl alcohol as an additive allows separation to occur but leads to a strong distortion of the chromatographic peaks. The application of basic additives such as isopropylamine or ammonia provides a stable separation with high selectivity and resolution as well as with the symmetrical peak shape. The selectivity coefficient is surprisingly almost independent of the amine modifier concentration in the mobile phase.

Key words

supercritical fluid chromatography salbutamol sulfate additive 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    D. Speybrouck, E. Lipka, J. Chromatogr. A, 2016, 1467, 33.CrossRefGoogle Scholar
  2. 2.
    A. Berthod, Chiral Recognition in Separation Methods: Mechanisms and Applications, Springer, Berlin, 2010, 337 pp.CrossRefGoogle Scholar
  3. 3.
    D. Speybrouck, C. Doublet, P. Cardinael, C. Fiol-Petit, D. Corens, J. Chromatogr. A, 2017, 1510, 89.CrossRefGoogle Scholar
  4. 4.
    K. De Klerck, D. Mangelings, D. Clicq, F. De Boever, Y. Vander-Heyden, J. Chromatogr. A, 2012, 1234, 72.CrossRefGoogle Scholar
  5. 5.
    M. Garzotti, M. Hamdan, J. Chromatogr. B, 2002, 770, 53.aaaaaCrossRefGoogle Scholar
  6. 6.
    O. I. Pokrovskiy, A. S. Kayda, O. I. Usovich, O. O. Parenago, V. V. Lunin, Russ. J. Phys. Chem., 2017, 91, 2288.CrossRefGoogle Scholar
  7. 7.
    O. I. Pokrovskiy, M. O. Kostenko, V. V. Lunin, Russ. Chem. Bull., 2017, 66, 2190.CrossRefGoogle Scholar
  8. 8.
    M. O. Kostenko, K. B. Ustinovich, O. I. Pokrovskiy, O. O. Parenago, N. G. Bazarnova, V. V. Lunin, Sverkhkriticheskie flyuidy: teoriya i praktika [Supercritical Fluids: Theory and Practice], 2017, 12, No. 4, 24 (in Russian).Google Scholar
  9. 9.
    V. A. Davankov, Khromatografiya. Osnovnye ponyatiya. Terminologiya [Chromatography. The Main Concepts. Terminology], Sborniki Nauchno-normativnoi Terminologii (Collections of Scientific Normative Terminology], Moscow, 1997, 48 pp. (in Russian).Google Scholar
  10. 10.
    Gosudarstvennaya Farmakopeya Rossiiskoi Federatsii [State Pharmacopeia of the Russian Federation], 13th ed., Moscow, 2015, 1, p. 452 (in Russian).Google Scholar
  11. 11.
    A. Cazenave-Gassiot, R. Boughtflower, J. Caldwell, L. Hitzel, C. Holyoak, S. Lane, P. Oakley, F. Pullen, S. Richardson, G. J. Langley, J. Chromatogr. A, 2009, 1216, 6441.CrossRefGoogle Scholar
  12. 12.
    R. Geryk, K. Kalikova, M. G. Schmidt, E. Tesarova, Anal. Chim. Acta, 2016, 932, 98.CrossRefGoogle Scholar
  13. 13.
    A. Lancuski, G. Vasilyev, J.-L. Putaux, E. Zussman, Biomacromol., 2015, 16, 2529.CrossRefGoogle Scholar
  14. 14.
    K. S. W. Sing, Pure Appl. Chem., 1982, 54, 2201.CrossRefGoogle Scholar
  15. 15.
    R. Arnell, P. Forssen, T. Forstedt, Anal. Chem., 2007, 79, 5838.CrossRefGoogle Scholar
  16. 16.
    F. Gritti, J. Chromatogr. A., 2016, 1468, 209.CrossRefGoogle Scholar
  17. 17.
    R. D. Oparin, M. A. Krestyaninov, E. A. Vorobyev, O. I. Pokrov skiy, O. O. Parenago, M. G. Kiselev, J. Mol. Liq., 2017, 239, 83.CrossRefGoogle Scholar
  18. 18.
    P. G. Jessop, D. J. Heldebrant, X. Li, C. A. Eckert, C. L. Liotta, Nature, 2005, 436, 1102.CrossRefGoogle Scholar
  19. 19.
    R. Arnell, P. Forssen, T. Fornstedt, Anal. Chem., 2007, 79, 5838.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • M. O. Kostenko
    • 1
    • 2
  • O. I. Pokrovskiy
    • 1
  • O. O. Parenago
    • 1
    • 2
  • V. V. Lunin
    • 1
    • 2
  1. 1.N. S. Kurnakov Institute of General and Inorganic ChemistryRussian Academy of SciencesMoscowRussian Federation
  2. 2.Chemical DepartmentM. V. Lomonosov Moscow State University, Building 3MoscowRussian Federation

Personalised recommendations