Russian Chemical Bulletin

, Volume 59, Issue 1, pp 292–296 | Cite as

Mass spectrometric monitoring of exhaled breath condensate proteome of a patient after lung transplantation

  • V. S. Kurova
  • E. C. Anaev
  • A. S. Kononikhin
  • I. A. Popov
  • K. Yu. Fedorchenko
  • E. N. Nikolaev
  • S. D. Varfolomeev
  • A. G. Chuchalin
Brief Communications
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Abstract

Analysis of samples of exhaled breath condensate (EBC) is a promising noninvasive method for the control of the status of the human respiratory system. In the present work, the proteome of the EBC samples received from a patient with lung dystrophy at different times before and after bilateral lung transplantation is analyzed by ion cyclotron resonance mass spectrometry. Qualitative protein composition of EBC samples obtained during the first month correlates with the clinical data on the acceptance of the transplanted lungs (allograft). Fifteen months after the lung surgery, the protein spectrum was similar to the normal composition of EBC proteins. This result agrees with the medical conclusion about normal lung functioning. The results suggest that the mass spectrometric monitoring of the protein spectra of EBC may be a tool for noninvasive pulmonological diagnostics.

Key words ion cyclotron resonance mass spectrometry proteomic analysis exhaled breath condensate diagnostics of respiratory diseases 
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Copyright information

© Springer Science+Business Media, Inc.  2010

Authors and Affiliations

  • V. S. Kurova
    • 1
  • E. C. Anaev
    • 2
  • A. S. Kononikhin
    • 1
    • 3
  • I. A. Popov
    • 1
  • K. Yu. Fedorchenko
    • 1
  • E. N. Nikolaev
    • 1
  • S. D. Varfolomeev
    • 1
  • A. G. Chuchalin
    • 2
  1. 1.N. M. Emanuel Institute of Biochemical Physics Russian Academy of SciencesMoscowRussian Federation
  2. 2.Research Institute of PulmonologyFederal Medicobiological Academy of RussiaMoscowRussian Federation
  3. 3.Institute for Energy Problems of Chemical PhisicsRussian Academy of SciencesMoscowRussian Federation

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