Research on Chemical Intermediates

, Volume 45, Issue 1, pp 23–32 | Cite as

Controlled release of DNA from zinc and magnesium ion-doped hydroxyapatites

  • Chie KojimaEmail author
  • Kenji Watanabe
  • Hidenobu Murata
  • Yuki Nishio
  • Rie Makiura
  • Katsuyuki Matsunaga
  • Atsushi Nakahira


Hydroxyapatite (HA) is a biocompatible and porous material that is useful for gene delivery. In this study, various Zn- and Mg-doped HA samples were produced by adding the ions at different dopant concentrations to control the association and dissociation of DNA. Although the HA crystal structure was retained after doping, it became distorted with increasing doped metal concentration. In 20% Zn-doped HA, a secondary Zn(OH)2 phase was observed. Mg-doped HA did not show significant cytotoxicity against breast tumor cells and osteoblasts. But, highly Zn-doped HAs were toxic, suggesting that doped Zn ions affected the cells. DNA could effectively adsorb to HA, regardless of metal ion doping, but the dissociation behavior of DNA differed. DNA was gradually released from Mg-doped HA for over a week, independent of Mg dope concentration, but not from Zn-doped HA, except for 20% doping. Therefore, the release properties of HA can be controlled by doping with suitable metal ion species at an appropriate dosage.


Hydroxyapatite DNA Dope Magnesium ion Zinc ion Controlled release 



This work was supported in part by the Elements Science and Technology Project from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT).


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Chie Kojima
    • 1
    Email author
  • Kenji Watanabe
    • 1
  • Hidenobu Murata
    • 2
  • Yuki Nishio
    • 3
  • Rie Makiura
    • 3
  • Katsuyuki Matsunaga
    • 4
  • Atsushi Nakahira
    • 3
  1. 1.Department of Applied Chemistry, Graduate School of EngineeringOsaka Prefecture UniversitySakaiJapan
  2. 2.Department of Material Science and Engineering, Graduate School of EngineeringKyoto UniversityKyotoJapan
  3. 3.Department of Materials Science, Graduate School of EngineeringOsaka Prefecture UniversitySakaiJapan
  4. 4.Department of Materials Physics, Graduate School of EngineeringNagoya UniversityNagoyaJapan

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