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Research on Chemical Intermediates

, Volume 41, Issue 9, pp 6707–6720 | Cite as

Use of partial least-squares discriminant analysis to study the effects of gradual scale-up of culture, and optimization of bioreactor angle and aeration volume on culture of Panax quinquefolium L. adventitious roots in a 5-L balloon-type bubble bioreactor

  • Qin Wang
  • Juan Wang
  • Jing Li
  • Shuli Man
  • Wenyuan Gao
Article
  • 141 Downloads

Abstract

Gradual scale-up culture of adventitious roots of Panax quinquefolium L. has been successfully achieved.Production of ginsenosides and polysaccharides was enhanced in a bioreactor compared with flask-type culture. The conditions for culture of adventitious roots were optimized.Partial least-squares discriminant analysis of the results showed growth rate and amounts of some compounds correlated positively with use of a 5-L balloon-type bubble bioreactor at an angle of 90° and with an aeration volume of 0.5 v/v/min.

Keywords

Panax quinquefolium PLS-DA Polysaccharide Ginsenoside Adventitious roots Bioreactor 

Abbreviations

MS

Murashige and Skoog (1962)

2,4-D

2,4-Dichlorophenoxyacetic acid

IBA

Indole-3-butyric acid

NAA

α-Naphthylacetic acid

BTBB

Balloon-type bubble bioreactor

DW

Dry weight

FW

Fresh weight

EC

Electrical conductivity

SOUR

Specific oxygen uptake ratio

PLS-DA

Partial least-squares discriminant analysis

Notes

Acknowledgments

This research was funded by Tianjin University Innovation Fund, China (2013XQ0046) and the Central Significant Increase or Decrease Program, China (ID: 20603020302).

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Key Laboratory of Industrial Fermentation Microbiology, Ministry of EducationTianjin University of Science and TechnologyTianjinChina
  2. 2.Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and TechnologyTianjin UniversityTianjinChina

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