Reviews in Fish Biology and Fisheries

, Volume 26, Issue 1, pp 93–108 | Cite as

Wide-targeted gene expression infers tissue-specific molecular signatures of lipid metabolism in fed and fasted fish

  • Simona Rimoldi
  • Laura Benedito-Palos
  • Genciana Terova
  • Jaume Pérez-Sánchez
Research Paper

Abstract

European sea bass (Dicentrarchus labrax) is one of the most important species for Mediterranean aquaculture. It is therefore crucial to establish appropriate feeding management regimens and to gain better knowledge of nutritional requirements for this species, exploring not only new feed ingredients and feeding strategies, but also understanding the molecular mechanisms that regulate the metabolism of nutrients. Accordingly, transcriptomic analysis represents a useful nutrigenomic discovery tool for identifying the molecular basis of biological responses to nutrition as well as nutritional biomarkers in fish. This study evaluated how the transcriptional activity of genes controlling lipid metabolism in European sea bass were modulated in a tissue-specific manner in response to fasting and refeeding. Such approach focused on a panel of 29 genes in which desaturases, elongases, triacylglycerol lipases, fatty acid-binding proteins, β-oxidation and oxidative phosphorylation enzymes, phospholipid-related enzymes, and transcription factors that regulate lipid homeostasis were represented. Fasting activated the lipolytic machinery in adipose tissue, liver and muscle of European sea bass, whereas markers of lipogenesis were downregulated in liver and adipose tissue. Genes involved in phospholipid and oxidative metabolism were differentially regulated in liver and skeletal muscle of fasted European sea bass. However, 12 days of refeeding were sufficient, for the most part, to reverse the expression of key genes. Overall, our data clearly showed a tissue-specific regulation of lipid-related genes according to the different metabolic capabilities of each tissue, being the brain the most refractory organ to changes in nutrient and energy availability and liver the most responsive tissue.

Keywords

Aquaculture Transcriptome Lipid metabolism Fasting and refeeding Biomarkers 

Supplementary material

11160_2015_9408_MOESM1_ESM.docx (46 kb)
Supplementary material 1 (DOCX 45 kb)

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Copyright information

© Springer International Publishing Switzerland 2015

Authors and Affiliations

  • Simona Rimoldi
    • 1
  • Laura Benedito-Palos
    • 3
  • Genciana Terova
    • 1
    • 2
  • Jaume Pérez-Sánchez
    • 3
  1. 1.Department of Biotechnology and Life Sciences (DBSV)University of InsubriaVareseItaly
  2. 2.Inter-University Centre for Research in Protein Biotechnologies “The Protein Factory”Polytechnic University of Milan and University of InsubriaVareseItaly
  3. 3.Nutrigenomics and Fish Growth Endocrinology GroupIATS-CSIC - Institute of Aquaculture Torre de la SalTorre de la SalSpain

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