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Reviews in Fish Biology and Fisheries

, Volume 25, Issue 1, pp 231–244 | Cite as

Diversity of the Astyanax scabripinnis species complex (Teleostei: Characidae) in the Atlantic Forest, Brazil: species limits and evolutionary inferences

  • Jonathan Pena Castro
  • Mauricio Osvaldo Moura
  • Orlando Moreira-Filho
  • Oscar Akio Shibatta
  • Mateus Henrique Santos
  • Viviane Nogaroto
  • Marcelo Ricardo Vicari
  • Mara Cristina de Almeida
  • Roberto Ferreira ArtoniEmail author
Research Paper

Abstract

The Astyanax scabripinnis species complex with its wide geographical distribution is an excellent model for evolutionary studies. Populations are usually geographically isolated but also, in some cases, occur in sympatry. In this study, five allopatric and/or sympatric populations of A. scabripinnis were analysed using geometric morphometry, cytogenetic markers, assays for induced breeding and phylogenetic inferences to draw conclusions on species limits and speciation processes in a natural setting. The morphometry of individuals indicated that the populations were well differentiated from each other. Cytogenetic evidence revealed a more conserved karyotypic macrostructure; however, molecular cytogenetic results obtained by in situ hybridization indicated 5S and 18S rDNA gene probe locations specific to each population. The reproduction tests for three locations suggest isolation between populations and the phylogenetic analyses suggest that the fish evaluated cluster in a monophyletic group. The combined data indicate that individuals are adapted to different environments in a complex evolutionary scenario, with linkage of populations during a recent geological period. However, due to reproductive isolation, the populations are evolving independently, reinforcing the existence of distinct cryptic species.

Keywords

Fish Biodiversity Climate variability Evolutionary analysis Geometric morphometry Cytogenetic 

Notes

Acknowledgments

The authors are grateful to the Instituto Chico Mendes de Conservação da Biodiversidade (ICMBio). This study was financed by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and the Fundação Araucária (Fundação Araucária de Apoio ao Desenvolvimento Científico e Tecnológico do Estado do Paraná).

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Copyright information

© Springer International Publishing Switzerland 2014

Authors and Affiliations

  • Jonathan Pena Castro
    • 1
  • Mauricio Osvaldo Moura
    • 2
  • Orlando Moreira-Filho
    • 3
  • Oscar Akio Shibatta
    • 4
  • Mateus Henrique Santos
    • 1
  • Viviane Nogaroto
    • 1
  • Marcelo Ricardo Vicari
    • 1
  • Mara Cristina de Almeida
    • 1
  • Roberto Ferreira Artoni
    • 1
    Email author
  1. 1.Programa de Pós Graduação em Biologia Evolutiva, Departamento de Biologia Estrutural, Molecular e GenéticaUniversidade Estadual de Ponta GrossaPonta GrossaBrazil
  2. 2.Departamento de Zoologia, Centro PolitécnicoUniversidade Federal do ParanáCuritibaBrazil
  3. 3.Departamento de Genética e EvoluçãoUniversidade Federal de São CarlosSão CarlosBrazil
  4. 4.Departamento de Biologia Animal e Vegetal, Centro de Ciências BiológicasUniversidade Estadual de LondrinaLondrinaBrazil

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