Reviews in Fish Biology and Fisheries

, Volume 22, Issue 1, pp 11–16 | Cite as

Microflora in fish digestive tract plays significant role in digestion and metabolism

  • S. GangulyEmail author
  • A. Prasad


Microflora of the environment plays an important role in the formation of the microflora of the digestive tract of fishes (Strom and Olafsen Microbiology of poecilotherms Elsevier, Amsterdam, pp 181–185 1990; Hansen et al. Appl Environ Microbiol 58:461–470 1992). Compared to water, digestive tract is an ecosystem far richer in nutrients and therefore more favorable for the growth of the majority of bacteria. Definitely, not all bacteria in food which gain entry in the digestive tract of fishes establish themselves there (Yan-Bo Wang et al. Aquaculture 281(1–4): 1–4 2008). Part of them adapts themselves in the digestive tract, whereas the others are digested by the enzymes produced by the host organism. Microorganisms present in the digestive tract feed on the food of the host organism which is digested by the enzymes produced by them and by the latter. ‘Chymous’ gets formed which decides the abundance and qualitative composition of microorganisms present in digestive tract.


Bacteria Digestive tract Fishes Microflora Microorganisms 


  1. Bairagi A, Ghosh KS, Sen SK, Ray AK (2002) Enzyme producing bacterial flora isolated from fish digestive tracts. Aquac Int 10:109–121CrossRefGoogle Scholar
  2. Barr B, Hsieh YL, Ganem BW (1996) Identification of two functionally different classes of exocellulases. Biochemistry 35:586–592PubMedCrossRefGoogle Scholar
  3. Cahill MM (1990) Bacterial flora of fishes: a review. Microbiol Ecol 19:21–41CrossRefGoogle Scholar
  4. Davies ME (1965) Cellulolytic bacteria in some ruminants and herbivores as shown by fluorescent antibody. J Gen Microbiol 39:139–141PubMedGoogle Scholar
  5. Finegold GM, Sutter VL, Mathisen GE (1983) Normal indigenous intestinal flora. In: Hentgens DJ (ed) Human intestinal microflora in health and disease. NewYork, Academic Press, pp 3–31Google Scholar
  6. Furuichi M, Yone Y (1982) Availability of carbohydrate in nutrition of carp and red sea bream. Bull Jpn Soc Sci Fisheries 48:945–948CrossRefGoogle Scholar
  7. Ganguly S, Paul I, Mukhopadhayay SK (2010). Immunostimulant, probiotic and prebiotic—their applications and effectiveness in aquaculture. Israeli J. Aquacult—Bamidgeh. 62(3): 130–138Google Scholar
  8. Ghosh K, Sen SK, Ray AK (2002) Characterization of Bacilli isolated from the gut of rohu, Labeo rohita, fingerlings and its significance in digestion. J Appl Aquac 12:33–42CrossRefGoogle Scholar
  9. Ghosh K, Roy M, Kar N, Ringo E (2010) Gastrointestinal bacteria in rohu, Labeo rohita (Actinopterygii: Cypriniformes: Cyprinidae): scanning electron microscopy and bacteriological study. Acta Ichthyol. Piscat 40(2):129–135CrossRefGoogle Scholar
  10. Gildberg A, Mikkelsen H, Sandaker E, Ringo D (1997) Probiotic effect of lactic acid bacteria in the feed on growth and survival of fry of Atlantic cod (Gadus morhua). Hydrobiologia 352:279–285CrossRefGoogle Scholar
  11. Hansen GH, Strom E, Olafsen JA (1992) Effect of different holding regimes on the intestinal microflora of herring (Clupea harengus) larvae. Appl Environ Microbiol 58:461–470PubMedGoogle Scholar
  12. Kristensen JH (1972) Carbohydrases of some marine invertebrates with notes on their food and on the natural occurrence of carbohydrates studied. Marine Biol 14:130–142CrossRefGoogle Scholar
  13. Leahy JG, Colwell RR (1990) Microbial degradation of hydrocarbons in the environment. Microbiol Rev 54(3):305–315PubMedGoogle Scholar
  14. Lesel R (1991) Does a digestive active bacterial flora exist in fish? In: Fish Nutrition in Practice. Biarritz, France, pp 655–664Google Scholar
  15. Lesel R, Fromageot C, Lesel M (1986) Cellulose digestibility in grass carp and goldfish. Aquaculture 54:11–17CrossRefGoogle Scholar
  16. Lindsay GJH, Harris JE (1980) Carboxymethylcellulase activity in the digestive tracts of fish. J Fish Biol 16:219–233CrossRefGoogle Scholar
  17. Meisner A, Burns J (1997) Viviparity in the Halfbeak Genera Dermogenys and Nomorhamphus (Teleostei: Hemiramphidae). J Morphol 234:295–317CrossRefGoogle Scholar
  18. Mondal S, Roy T, Sen SK, Ray AK (2008) Distribution of enzyme-producing bacteria in the digestive tracts of some freshwater fish. Acta Ichthyologica et Piscatoria 38(1):1–8CrossRefGoogle Scholar
  19. Pucci OH, Bak MA, Peressutti SR, Klein I, Hartig C, Alvarez HM, Wünsche L (2004) Influence of crude oil contamination on the bacterial community of semiarid soils of Patagonia (Argentina). Acta Biotechnol 20(2):129–146CrossRefGoogle Scholar
  20. Ray AK, Roy T, Mondal S, Ringo E (2009) Identification of gut-associated amylase, cellulase and protease-producing bacteria in three species of Indian major carps. Aquac Res 41(10):1462–1469Google Scholar
  21. Ringo E, Birkbeck TH (1999) Intestinal microflora of fish and fry: a review. Aquac Res 30(2):73–93CrossRefGoogle Scholar
  22. Ringo E, Olsen RE (1999) The effect of diet on aerobic bacterial flora associated with intestine of Arctic charr (Salvelinus alpinus). J Appl Microbiol 86:22–28PubMedCrossRefGoogle Scholar
  23. Ringo E, Strom E (1994) Microflora of Arctic charr Salvelinus alpinus gastrointestinal microflora of freeliving fish and effect of diet and salinity on the intestinal microflora. Aquac Fish Mgt 25:623–629Google Scholar
  24. Saha AK, Ray AK (1998) Cellulase activity in rohu fingerlings. Aquac Int 6:281–291CrossRefGoogle Scholar
  25. Saha S, Roy RN, Sen SK, Roy AK (2006) Characterization of cellulase-producing bacteria from the digestive tract of tilapia, Oreochromis mossambica (Peters) and grass carp, Ctenopharyngodon idella (Valencinnes). Aquac Res 37:380–388CrossRefGoogle Scholar
  26. Sakata T (1990) Microflora in the digestive tract of fish and shellfish. In: Lesel R (ed) Microbiology of Poekilotherms. Elsevier, Amsterdam, pp 217–223Google Scholar
  27. Sakata T, Yuki T (1992) Diagnostic media for differentiation of Plesiomonas from intestinal microflora of freshwater fish. Bull Jpn Soc Sci Fish 58(5):977–979Google Scholar
  28. Savas S, Kubilay A, Basmaz N (2005) Effect of bacterial load in feeds on intestinal microflora of seabream (Sparus aurata) larvae and juveniles Israeli. J Aquac—Bamidgeh 57(1):3–9Google Scholar
  29. Scott P (1997). Livebearing Fishes. pp. 13. Tetra PressGoogle Scholar
  30. Shiau Shi Yen (1997) Utilization of carbohydrates in warmwater fish—with particular reference to tilapia, Oreochromis niloticus X O. aureus. Fish Nutrition and Feeding Proceedings of the Sixth International Symposium on Feeding and Nutrition in Fish. Aquaculture 151(1–4):79–96CrossRefGoogle Scholar
  31. Shiau Shi-Yen, Hao-Ling Yu, Hwa Sophy, Chen Shih-Yang, Hsu Song-Ing (1988) The influence of carboxymethylcellulose on growth, digestion, gastric emptying time and body composition of tilapia. Aquaculture 70(4):345–354CrossRefGoogle Scholar
  32. Shiina T, Shimizu S, Hosomichi K, Kohara S, Watanabe S, Hanzawa K, Beck S, Kulski JK, Inoko H (2004) Comparative genomic analysis of two avian (quail and chicken) MHC regions. J Immunol 172:6751–6763PubMedGoogle Scholar
  33. Smith LS (1989) Digestive functions in teleost fishes. In: Halver JE (ed) Fish Nutririon, 2nd edn. Academic Press, San Diego, pp 331–421Google Scholar
  34. Spano L, Medeiros J, Mandels M (1975) Enzymatic hydrolysis of cellulosic waste to glucose. Pollution Abatement Division, Food Services Laboratories, US Army, Natick. Massachusetts, USAGoogle Scholar
  35. Stavric S, Kornegay T (1995) Microbial probiotics for pigs and poultry. In: Wallace RJ, Chesson A (eds) Biotechnlogy in animal feeds and animal feeding. Weinheim, NewYork, pp 205–231CrossRefGoogle Scholar
  36. Strom E, Olafsen JA (1990) The indigenous microflora of wild-captured juvenile cod in net-pen rearing. In: Lesel R (ed) Microbiology of poecilotherms. Elsevier, Amsterdam, pp 181–185Google Scholar
  37. Sugita H, Tsunohara M, Ohkashi T, Deguchi Y (1988) The establishment of an intestinal microflora in developing goldfish Carassius auratus ponds. Microbiol Ecol 15:333–344CrossRefGoogle Scholar
  38. Sugita H, Kawasahi J, Deguchi Y (1997) Production of amylase by the intestinal microflora in cultured freshwater fish. Lett Appl Microbiol 24:105–108PubMedCrossRefGoogle Scholar
  39. Syvokieni J (1989) Symbiotic digestion in hydrobionts and insects. Mokslas, VilniusGoogle Scholar
  40. Takeuchi T (1991) Digestion and Nutririon of Fish. In: Itazawa Y, Hanyu S, Tokyo I (ed) Fish Physiology: Koseisha Koseikaku Japan (in Japanese). pp. 67–101Google Scholar
  41. Trust TJ, Sparrow RAH (1974) The bacterial flora in the alimentary tract of freshwater salmonid fishes. Can J Microbiol 20:1219–1228PubMedCrossRefGoogle Scholar
  42. Vesta Skrodenyte-Arbaeiauskiene (2000) Proteolytic activity of the roach (Rutilus rutilus L.) intestinal microflora. Acta Zool Lituanica. 10: 3Google Scholar
  43. Voveriene G, Mickeniene L, Ðyvokiene J (2002) Hydrocarbon-degrading bacteria in the digestive tract of fish, their abundance, species composition, and activity. Acta Zool Lituanica. 12: 3Google Scholar
  44. Wang Yan-Bo, Rong-Li Jian, Lin Junda (2008) Probiotics in aquaculture: challenges and outlook. Aquaculture 281(1–4):1–4Google Scholar
  45. Westerdahl A, Olsen JC, Kjelleberg S, Comway P (1991) Isolation and characterization of turbot (Scophthalmus maimus) associated bacteria with inhibitory effects against Vibrio anguillarum. Appl Environ Microbiol 57:2223–2228PubMedGoogle Scholar

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© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  1. 1.All India Coordinated Research Project on Post Harvest Technology (Kolkata Centre), Department of Fish Processing Technology, Faculty of Fishery SciencesWest Bengal University of Animal and Fishery SciencesKolkataIndia
  2. 2.Department of Veterinary Microbiology, Faculty of Veterinary Science & Animal HusbandryBirsa Agricultural UniversityRanchiIndia

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