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The effects of egg position, egg mass size, substrate and biofouling on embryo mortality in the squid Sepioteuthis australis

  • M. A. SteerEmail author
  • N. A. Moltschaniwskyj
Original Paper

Abstract

Using a combination of laboratory and field investigations, this study examined embryo mortality in the southern calamary Sepioteuthis australis as a function of egg mass size, the substrate upon which the mass is attached, the position of the embryo within the mass, and the degree of biofouling. Egg mass size ranged from 2 to 1,241 egg strands, however most masses consisted of 200–299 strands. Small egg masses (<300 strands) were generally attached to soft-sediment vegetation (Amphibolis antarctica, Heterozostera tasmanica, Caulerpa sp.), whereas larger masses (>300 strands) were either securely attached to robust macroalgae holdfasts (Ecklonia sp., Marcocystis pyrifera, Sargassum sp.) or unattached. Rates of embryo mortality were highly variable ranging from 2 to 25%. Both laboratory and field results indicated a positive relationship between egg mass size and embryo mortality. Larger, unattached egg masses contained twice as many dead embryos than those securely attached to a substrate. Mortality rates were significantly affected by the embryos’ relative position within the mass and were highest in embryos located near the attachment point of the egg strand, within the interior of the mass, and in close contact with the substrate. This was attributed to the inability of the embryos to respire adequately and eliminate metabolic wastes. Biofouling did not strongly influence embryo mortality, but colonisation occurred in areas conducive to growth, photosynthesis, and respiration indicating ‘healthy’ regions within the mass.

Keywords

Embryo Mortality Substrate Egg mass Biofouling Calamary 

Notes

Acknowledgements

We thank G. Pecl, S. Tracey, D. Sinn, T. Jantzen and the crew of FRV “Challenger” for valuable field assistance, M. Haddon and P. Ziegler for statistical advice and B. McGrath-Steer, A. Jordan, J.R. Bower (Hokkaido University) and two anonymous reviewers for critical review of the manuscript. This research was carried out while M.A.S. was supported by a University of Tasmania Research Scholarship with supplementary funding from the Tasmanian Aquaculture and Fisheries Institute. All field expenses were met by a concurrent project funded by the FRDC (Fisheries Research and Development Corporation) (2000/121) awarded to N.A.M. This study complies with the current collection laws and code of ethics in Australia.

References

  1. Arkhipkin AI, Laptikhovsky VV, Middleton DAJ (2000) Adaptions for cold water spawning in loliginid squid: Loligo gahi in Falkland waters. J Moll Stud 66:551–564CrossRefGoogle Scholar
  2. Augustyn CJ (1990) Biological studies on the chokker squid Loligo vulgaris reynaudii (Cephalopoda; Myopsida) on spawning grounds off the south-east coast of South Africa. Sth Afr J Mar Sci 9:11–26Google Scholar
  3. Augustyn CJ, Lipinski MR, Roberts MJ, Mitchell-Innes BA (1994) Chokka squid on the Agulhas Bank: life history and ecology. Suid-Afrikaanse Tydskrif vir Wetenskap 90:143–153Google Scholar
  4. Benkendorff K (1999) Bioactive molluscan resources and their conservation: Biological and chemical studies on the egg masses of marine molluscs. Department of biological sciences and Department of chemistry. Wollongong, University of WollongongGoogle Scholar
  5. Biermann CH, Schinner GO, Strathmann RR (1992) Influence of solar radiation, microalgal fouling, and current on deposition site and survival of embryos of a dorid nudibranch gastropod. Mar Ecol Progr Ser 86:205–215Google Scholar
  6. Boletzky Sv (1986) Encapsulation of cephalopod embryos: a search for functional correlations. Amer Malac Bull 4(2):217–227Google Scholar
  7. Boletzky Sv (2003) Biology of early life stages in cephalopod molluscs. Adv Mar Biol 444:144–203Google Scholar
  8. Caddy JF (1983) The cephalopods: factors relevant to their population dynamics and to the assessment and management of stocks. In: Caddy JF (ed) Advances in assessment of world cephalopod resources. Rome, FAOGoogle Scholar
  9. Chaffee C, Strathmann RR (1984) Constraints on egg masses. I. Retarded development within thick egg masses J Exp Mar Biol Ecol 84:73–83CrossRefGoogle Scholar
  10. Choe S (1966) On the eggs, rearing, habits of the fry, and growth of some cephalopoda. Bull Mar Sci 16:330–348Google Scholar
  11. Cohen CS, Strathmann RR (1996) Embryos at the edge of tolerance: Effects of environment and structure of egg masses on supply of oxygen to embryos. Biol Bull 190:8–15CrossRefGoogle Scholar
  12. Cronin E, Seymour R (2000) Respiration of the eggs of the giant cuttlefish Sepia apama. Mar Biol 136:863–870CrossRefGoogle Scholar
  13. Edgar GJ (2000) Australian marine life: the plants and animals of temperate waters. Reed Books, Australia, 544 ppGoogle Scholar
  14. Gowland FC, Moltschaniwskyj NA, Steer MA (2002) Description and quantification of developmental abnormailities in a natural Sepioteuthis australis spawning population (Mollusca: Cephalopoda). Mar Ecol Progr Ser 243:133–141Google Scholar
  15. Griswold CA, Prezioso J (1981) In situ observations on reproductive behaviour of the long finned squid, Loligo pealei. Fish Bull 97:945–947Google Scholar
  16. Hanlon RT (1998) Mating systems and sexual selection in the squid Loligo: how might commercial fishing on spawning grounds affect them? CalCOFI Rep 39:92–99Google Scholar
  17. Jantzen TM, Havenhand JN (2002) Preliminary field observations of mating and spawning in the squid Sepioteuthis australis. Bull Mar Sci 71:1073–1080Google Scholar
  18. Jantzen TM, Havenhand JN (2003) Reproductive behaviour in the squid Sepioteuthis australis from South Australia: interactions on the spawning grounds. Biol Bull 204:305–317PubMedCrossRefGoogle Scholar
  19. McGowan JA (1954) Observations on the sexual behaviour and spawning of the squid, Loligo opalescens, at La Jolla, California. Calif Fish Game 40:47–54Google Scholar
  20. Moltschaniwskyj NA, Pecl GT, Lyle J (2002) An assessment of the use of short-term closures to protect spawning southern calamary aggregations from fishing pressure in Tasmania, Australia. Bull Mar Sci 71:501–514Google Scholar
  21. Moltschaniwskyj NA, Pecl GT (2003) Small-scale spatial and temporal patterns of egg production by the temperate loliginid Sepioteuthis australis. Mar Biol 142:509–516Google Scholar
  22. Moltschaniwskyj NA, Steer MA (2004) Spatial and seasonal variation in reproductive characterisitcs and spawning of southern calamary (Sepioteuthis australis): spreading the mortality risk. ICES J Mar Sci 61:921–927CrossRefGoogle Scholar
  23. Oosthuizen A, Roberts MJ, Sauer WHH (2002) Temperature effects on the embryonic development and hatching success of the squid Loligo vulgaris reynaudii. Bull Mar Sci 71:601–618Google Scholar
  24. Pechenik JA (1979) Role of encapsulation in invertebrate life histories. Amer Nat 114:859–870CrossRefGoogle Scholar
  25. Pechenik JA (1986) The encapsulation of eggs and embryos by molluscs: an overview. Amer Malac Bull 4(2):165–172Google Scholar
  26. Przeslawski R (2004) A review of the effects of environmental stress on embryonic development within intertidal gastropod eggs. Moll Res 24(1):43–63CrossRefGoogle Scholar
  27. Quinn GP, Keough MJ (2002) Experimental design and data analysis for biologists. Cambridge, UK, Cambridge University PressGoogle Scholar
  28. Sauer WHH, Smale MJ, Lipinski MR (1992) The location of spawning grounds, spawning and schooling behaviour of the squid Loligo vulgaris reynaudii (Cephalopoda: Myopsida) off the Eastern Cape Coast. Sth Afr J Mar Biol 114:97–107Google Scholar
  29. Sauer WHH, MacCarthy C, Smale MJ, Koorts A (1993) An investigation of the egg distribution of the chokka squid, Loligo vulgaris reynaudii, in Krom Bay, South Africa. Bull Mar Sci 53(3):1066–1077Google Scholar
  30. Sauer WHH, Roberts MJ, Lipinski MR, Smale MJ, Hanlon RT, Webber DM, O’Dor R (1997) Choreography of the squids “nuptial dance”. Biol Bull 192:203–207PubMedCrossRefGoogle Scholar
  31. Segawa S, Izuka T, Tamashiro T, Okutani T (1993) A note on mating and egg deposition by Sepioteuthis lessoniana in Ishigaki Island, Okinawa, Southwestern Japan. Venus 52(1):101–108Google Scholar
  32. Steer MA, Moltschaniwskyj NA, Jordan AR (2003) Embryonic development of the southern calamary Sepioteuthis australis within the constraints of an aggregated egg mass. Mar Freshw Res 54:217–226CrossRefGoogle Scholar
  33. Strathmann RR, Strathmann MF (1995) Oxygen supply and limits on aggregation of embryos. J Mar Biol Ass UK 75:413–428CrossRefGoogle Scholar
  34. Strathmann RR, Chaffee C (1984) Constraints on egg masses. II. Effect of spacing, size, and number of eggs on ventilation of masses of embryos in jelly, adherent groups, or thin-walled capsules. J Exp Mar Biol Ecol 84:85–93CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.School of Aquaculture, Tasmanian Aquaculture and Fisheries InstituteUniversity of TasmaniaLauncestonAustralia
  2. 2.South Australian Research and Development InstituteWest BeachAustralia

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