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Pomegranate Juice and Peel Extracts are Able to Inhibit Proliferation, Migration and Colony Formation of Prostate Cancer Cell Lines and Modulate the Akt/mTOR/S6K Signaling Pathway

Abstract

Pomegranate (Punica granatum) is known to contain polyphenols with many potential health benefits, including anti-tumoral, anti-inflammatory, and anti-microbial properties. It has been used in popular medicine for cancer treatment, which still represents the major cause of cancer-related deaths in men worldwide. Importantly, pomegranate peels are valuable by-products of the food industry that are rich in polyphenols. Here we report a comparison between juice and peel aqueous extracts in prostate cancer DU-145 and PC-3 cell lines. Both extracts were able to inhibit the proliferation, migration and colony formation of those cells, although peel extracts presented more robust effects compared to juice. Besides, the growth-related mTOR/S6K signaling pathway presented strong inhibition after pomegranate extracts treatment. This study presents evidence that both juice and isolated peel extracts from promegate fruit have important anti-cancer effects against prostate cancer cells, modulating the mTOR/S6K signaling pathway.

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Abbreviations

mTOR:

Mammalian Target Of Rapamycin

FBS:

Fetal bovine serum

PLC:

High-Performance Liquid Chromatography

TRAMP:

Transgenic adenocarcinoma of the mouse prostate

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Acknowledgements

This research has been funded by The São Paulo Research Foundation (grant numbers: FMS, 2012/13558-7 and 2018/14818-9; AECA, 2015/07299-7; RMNB, 2016/06457-0; MAR, 2013/04304-4; fellowship numbers: ICBP, 2015/003111; LBF, 2013/13002-1) and from CNPq (FMS, 447553/2014-3; MAR, 303568/2016-0).

Author information

FMC and LGS have performed cell culture experiments. ICBP has performed western blotting analysis. MAR has performed phenolics analysis. AECA, RMNB, and FMS have designed experiments and written the manuscript. The authors declare no conflict of interest.

Correspondence to Fernando Moreira Simabuco.

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Chaves, F.M., Pavan, I.C.B., da Silva, L.G.S. et al. Pomegranate Juice and Peel Extracts are Able to Inhibit Proliferation, Migration and Colony Formation of Prostate Cancer Cell Lines and Modulate the Akt/mTOR/S6K Signaling Pathway. Plant Foods Hum Nutr (2019). https://doi.org/10.1007/s11130-019-00776-0

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Keywords

  • Pomegranate
  • Peel extracts
  • Ellagic acid
  • Prostate cancer
  • mTOR
  • Cell signaling