Plant Foods for Human Nutrition

, Volume 73, Issue 3, pp 235–240 | Cite as

t-Resveratrol Protects against Acute High Glucose Damage in Endothelial Cells

  • Leda Guzmán
  • Cristóbal Balada
  • Guillermo Flores
  • Rocío Álvarez
  • Marcela Knox
  • Raúl Vinet
  • José L. MartínezEmail author
Original Paper


Postprandial hyperglycemia in diabetic and nondiabetic subjects is associated with endothelial dysfunction. Evidence shows that high glucose generates oxidative stress and a pro-inflammatory state promoting the development of cardiovascular diseases. trans-Resveratrol (t-RV) has been shown to reduce cardiovascular risk. To determine whether t-RV acts as a protector against acute high glucose (AHG)-induced damage, two in vitro models, rat aortic rings (RAR) and human umbilical vein endothelial cells (HUVEC) were used. RAR pretreated with AHG (25 mM D-glucose) for 3 h dramatically decreased the endothelium-dependent relaxation (EDR) induced by acetylcholine in phenylephrine (PE)-precontracted vessels. However, coincubation with t-RV significantly mitigated the damage induced by AHG on EDR. Pretreatment with AHG did not affect the vasodilation induced by sodium nitroprusside. HUVEC treated with t-RV decreased cytotoxicity and reduced radical oxygen species production induced by AHG. Taken together, these results suggest that t-RV can mitigate the AHG-induced EDR damage through a mechanism involving ROS scavenging and probably an increase in the bioavailability of NO.


Postprandial hyperglycemia Acute high glucose Oxidative stress Rat aorta Human umbilical vein endothelial cells Resveratrol 





Acute high glucose


The concentration of the compound that gives half-maximal response


Endothelium-dependent relaxation


Emax maximal effect (relaxation)


Human umbilical vein endothelial cells


Krebs-Henseleit buffer




Nitric oxide




Rat aortic rings


Reactive oxygen species


Sodium nitroprusside





This work was supported by Grants DIUV No. 57/2011 from Universidad de Valparaíso, Chile; Grant CREAS No. R12C1001 from CONICYT-REGIONAL, GORE Región de Valparaíso, Chile; Grant No. 037-274 from Pontificia Universidad Católica de Valparaíso, Chile and Grant DICYT-USACH No. 021643MS from JLM.

Compliance with Ethical Standards

Conflicts of Interest

The authors state no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Laboratory of Biological Chemistry, Institute of Chemistry, Faculty of SciencesPontificia Universidad Católica de ValparaísoValparaísoChile
  2. 2.Laboratory of Pharmacology, Faculty of PharmacyUniversidad de ValparaísoValparaísoChile
  3. 3.Centro de Investigación Farmacopea Chilena (CIFAR)ValparaísoChile
  4. 4.Regional Center for the Study in Foods and Health (CREAS)ValparaísoChile
  5. 5.Vice Presidency of Research, Development and InnovationUniversidad de Santiago de ChileSantiagoChile

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