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Plant Foods for Human Nutrition

, Volume 72, Issue 3, pp 288–293 | Cite as

Verifying Identities of Plant-Based Multivitamins Using Phytochemical Fingerprinting in Combination with Multiple Bioassays

  • Yeni Lim
  • Yoon Hee Ahn
  • Jae Keun Yoo
  • Kyoung Sik Park
  • Oran Kwon
Original Paper
  • 145 Downloads

Abstract

Sales of multivitamins have been growing rapidly and the concept of natural multivitamin, plant-based multivitamin, or both has been introduced in the market, leading consumers to anticipate additional health benefits from phytochemicals that accompany the vitamins. However, the lack of labeling requirements might lead to fraudulent claims. Therefore, the objective of this study was to develop a strategy to verify identity of plant-based multivitamins. Phytochemical fingerprinting was used to discriminate identities. In addition, multiple bioassays were performed to determine total antioxidant capacity. A statistical computation model was then used to measure contributions of phytochemicals and vitamins to antioxidant activities. Fifteen multivitamins were purchased from the local markets in Seoul, Korea and classified into three groups according to the number of plant ingredients. Pearson correlation analysis among antioxidant capacities, amount phenols, and number of plant ingredients revealed that ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picryhydrazyl (DPPH) assay results had the highest correlation with total phenol content. This suggests that FRAP and DPPH assays are useful for characterizing plant-derived multivitamins. Furthermore, net effect linear regression analysis confirmed that the contribution of phytochemicals to total antioxidant capacities was always relatively higher than that of vitamins. Taken together, the results suggest that phytochemical fingerprinting in combination with multiple bioassays could be used as a strategy to determine whether plant-derived multivitamins could provide additional health benefits beyond their nutritional value.

Keywords

Plant-based multivitamins Phytochemicals Fingerprinting Bioassay Natural claim 

Abbreviations

ABTS

2,2′-azino-di-[3-ethylbenzthiazoline sulphonate]

DPPH

2,2-diphenyl-1-picryhydrazyl

FRAP

Ferric reducing antioxidant power

GAE

Gallic acid equivalent

GPDIM

Generalized partially double-index model

HAT

Hydrogen atom transfer

ORAC

Oxygen radical absorbance capacity

Q-TOF

Quadrupole time-of-flight

TAC

Total antioxidant capacity

TAS

Total antioxidant status

TEAC

Trolox equivalent antioxidant capacity

TPC

Total phenolic content

Notes

Acknowledgements

This study was supported by Bio-synergy Research Project through the National Research Foundation (NRF 2012M3A9C4048761) funded by the Ministry of Science, ICT and Future Planning, Republic of Korea.

Compliance with Ethical Standards

Conflict of Interest

The authors have no conflicts of interest to declare.

Supplementary material

11130_2017_622_MOESM1_ESM.docx (33 kb)
ESM 1 (DOCX 32 kb)

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Department of Nutritional Science and Food ManagementEwha Womans UniversitySeoulRepublic of Korea
  2. 2.Department of StatisticsEwha Womans UniversitySeoulRepublic of Korea
  3. 3.Department of Biomedical ScienceCheongju UniversityCheongjuRepublic of Korea

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