Plant Foods for Human Nutrition

, Volume 71, Issue 1, pp 28–34 | Cite as

Effect of Allium flavum L. and Allium melanantherum Panč. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase

  • Dragana Mitić-Ćulafić
  • Biljana Nikolić
  • Nataša Simin
  • Nebojša Jasnić
  • Dragana Četojević-Simin
  • Maja Krstić
  • Jelena Knežević-Vukčević
Original Paper


Allium flavum L. and Allium melanantherum Panč. are wild growing plants used in traditional diet in Balkan region. While chemical composition and some biological activities of A. flavum have been reported, A. melanantherum, as an endemic in the Balkan Peninsula, has never been comprehensively examined. After chemical characterization of A. melanantherum, we examined the protective effect of methanol extracts of both species against t-butyl hydro-peroxide (t-BOOH)-induced DNA damage and mutagenesis. The bacterial reverse mutation assay was performed on Escherichia coli WP2 oxyR strain. DNA damage was monitored in human fetal lung fibroblasts (MRC-5) with alkaline comet assay. Obtained results indicated that extracts reduced t-BOOH-induced DNA damage up to 70 and 72 % for A. flavum and A. melanantherum extract, respectively, and showed no effect on t-BOOH-induced mutagenesis. Since the results indicated modulatory effect on cell-mediated antioxidative defense, the effect of extracts on total protein content, and superoxide dismutase (SOD) and catalase (CAT) amounts and activities were monitored. Both extracts increased total protein content, while the increase of enzyme amount and activity was obtained only with A. melanantherum extract and restricted to CAT. The activity of CuZnSOD family was not affected, while SOD1 and SOD2 amounts were significantly decreased, indicating potential involvement of extracellular CuZnSOD. Obtained results strongly support the traditional use of A. flavum and A. melanantherum in nutrition and recommend them for further study.


Allium flavum Allium melanantherum Antigenotoxicity Oxidative DNA damage Antioxidative enzymes 





reactive oxygen species


superoxide dismutase


t-butyl hydroperoxide



This work was supported by the Ministry of Science of Republic of Serbia, Project No. 172058.

Compliance with Ethical Standards

Conflict of Interest

The authors declare no conflict of interest.

Supplementary material

11130_2015_519_MOESM1_ESM.doc (98 kb)
Supplementary Table 1 (DOC 98 kb)
11130_2015_519_MOESM2_ESM.doc (28 kb)
Supplementary Table 2 (DOC 27 kb)
11130_2015_519_MOESM3_ESM.doc (28 kb)
Supplementary Table 3 (DOC 28 kb)


  1. 1.
    Olinski R, Gackowski D, Foksinski M, et al. (2002) Oxidative DNA damage: assessment of the role in carcinogenesis, atherosclerosis, and acquired immunodeficiency syndrome. Free Radic Biol Med 33:192–200CrossRefGoogle Scholar
  2. 2.
    Cooke MS, Evans MD, Dizdaroglu M, Lunec J (2003) Oxidative DNA damage: mechanisms, mutation, and disease. FASEB J 17:1195–1214CrossRefGoogle Scholar
  3. 3.
    Morihara N, Hayama M, Fujii H (2011) Aged garlic extract scavenges superoxide radicals. Plant Foods Hum Nutr 66:17–21CrossRefGoogle Scholar
  4. 4.
    Dimitrios B (2006) Sources of natural phenolic antioxidants. Trends Food Sci Technol 17:505–512CrossRefGoogle Scholar
  5. 5.
    Ziech D, Anestopoulos I, Hanafi R, et al. (2012) Pleiotropic effects of natural products in ROS-induced carcinogenesis: the role of plant-derived natural products in oral cancer chemoprevention. Cancer Lett 327:16–25CrossRefGoogle Scholar
  6. 6.
    Keusgen M (2002) Health and alliums. In: Rabinowitch HD, Currah L (eds) Allium crop science: recent advances. CABI Publishing, New York, pp. 357–378CrossRefGoogle Scholar
  7. 7.
    Thomson M, Ali M (2003) Garlic (Allium sativum): a review of its potential use as an anti-cancer agent. Curr Cancer Drug Tar 3:67–81CrossRefGoogle Scholar
  8. 8.
    Alpers DH (2009) Garlic and its potential for prevention of colorectal cancer and other conditions. Curr Opin Gastroen 25:116–121CrossRefGoogle Scholar
  9. 9.
    Park JH, Park YK, Park E (2009) Antioxidative and antigenotoxic effects of garlic (Allium sativum L.) prepared by different processing methods. Plant Foods Hum Nutr 64:244–249CrossRefGoogle Scholar
  10. 10.
    Votto APS, Domingues BS, de Souza MM, et al. (2010) Toxicity mechanisms of onion (Allium cepa) extracts and compounds in multidrug resistant erythroleukemic cell line. Biol Res 43:429–438CrossRefGoogle Scholar
  11. 11.
    Simin N, Orčič D, Ćetojević-Simin D, et al. (2013) Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae). LWT Food Sci Technol 54:139–146CrossRefGoogle Scholar
  12. 12.
    Stearn WT (1980) Allium L. In: Tutin TG, Heywood VH, Burges NA, Moore DM, Valentine DH, Walters SM, Webb DA (eds) Flora europaea V. Cambrdge University Press, Cambridge, UK, pp. 49–69Google Scholar
  13. 13.
    Blanco M, Urios A, Martinez A (1998) New Escherichia coli WP2 tester strains highly sensitive to reversion by oxidative mutagens. Mutat Res 413:95–101CrossRefGoogle Scholar
  14. 14.
    Četojević-Simin DD, Velićanski AS, Cvetković DD, et al. (2012) Bioactivity of lemon balm kombucha. Food Bioprocess Tech 5:1756–1765CrossRefGoogle Scholar
  15. 15.
    Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175:184–191CrossRefGoogle Scholar
  16. 16.
    Nikolić B, Mitić-Ćulafić D, Vuković-Gačić B, Knežević-Vukčević J (2011) Modulation of genotoxicity and DNA repair by plant monoterpenes camphor, eucalyptol and thujone in E. coli and mammalian cells. Food Chem Toxicol 49:2035–2045CrossRefGoogle Scholar
  17. 17.
    Mitić-Ćulafić D, Žegura B, Nikolić B, et al. (2009) Protective effect of linalool, myrcene and eucalyptol against t-butyl hydroperoxide induced genotoxicity in bacteria and cultured human cells. Food Chem Toxicol 47:260–266CrossRefGoogle Scholar
  18. 18.
    Weydert JC, Cullen JJ (2010) Measurement of superoxide dismutase, catalase, and glutathione peroxidase in cultured cells and tissue. Nat Protoc 5:51–66CrossRefGoogle Scholar
  19. 19.
    Schneider CA, Rasband WS, Eliceiri KW (2012) NIH image to ImageJ: 25 years of image analysis. Nat Methods 9:671–675CrossRefGoogle Scholar
  20. 20.
    Claiborne A (1985) Catalase activity. In: Greenwald RA (ed) Handbook of methods for oxygen free radical research. CRC Press, Boca Raton, FL, pp. 273–284Google Scholar
  21. 21.
    Burnette WN (1981) "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate–polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem 112:195–203CrossRefGoogle Scholar
  22. 22.
    Simin N (2014) Secondary metabolites from selected species of genus Allium sect. Codoprasum Rchb. – biological activities, phytochemical and chemotaxonomic aspects. Dissertation (in Serbian), University of Novi Sad, SerbiaGoogle Scholar
  23. 23.
    Urios A, Blanco M (1996) Specificity of spontaneous and t-butyl hydroperoxide-induced mutations in ΔoxyR strains of Escherichia coli differing with respect to the SOS mutagenesis proficiency and to the MutY and MutM functions. Mutat Res 354:95–101CrossRefGoogle Scholar
  24. 24.
    Babich H, Schuck AG, Weisburg JH, Zuckerbraun HL (2011) Research strategies in the study of the pro-oxidant nature of polyphenol nutraceuticals. J Tox. ID 467305, 12 pages, doi: 10.1155/2011/467305.
  25. 25.
    Das NU (2002) A radical approach to cancer. Med Sci Monit 8:RA79–RA92Google Scholar
  26. 26.
    Davies AJK (2000) Oxidative stress, antioxidant defenses, and damage removal, repair, and replacement system. IUBMB Life 50:279–289CrossRefGoogle Scholar
  27. 27.
    Wang B-S, Huang G-J, Lu Y-H, Chang L-W (2013) Anti-inflammatory effects of an aqueous extract of Welsh onion green leaves in mice. Food Chem 138:751–756CrossRefGoogle Scholar
  28. 28.
    Helen A, Krishnakumar K, Vijayammal PL, Augusti KT (2000) Antioxidant effect of onion oil (Allium cepa Linn) on the damages induced by nicotine in rats as compared to alpha-tocopherol. Toxicol Lett 116:61–68CrossRefGoogle Scholar
  29. 29.
    El-Demerdash FM, Yousef MI, El-Naga NIA (2005) Biochemical study on the hypoglycemic effects of onion and garlic in alloxan-induced diabetic rats. Food Chem Toxicol 43:57–63CrossRefGoogle Scholar
  30. 30.
    Yeh CT, Yen GC (2006) Induction of hepatic antioxidant enzymes by phenolic acids in rats is accompanied by increased levels of multidrug resistance-associated protein 3 mRNA expression. J Nutr 36:11–15Google Scholar
  31. 31.
    Patil SL, Mallaaiah SH, Patil RK (2013) Antioxidative and radioprotective potential of rutin and quercetin in Swiss albino mice exposed to gamma radiation. J Med Phys 38:87–92CrossRefGoogle Scholar
  32. 32.
    Nencini C, Menchiari A, Franchi GG, Micheli L (2011) In vitro antioxidant activity of aged extracts of some Italian Allium species. Plant Foods Hum Nutr 66:11–16CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Dragana Mitić-Ćulafić
    • 1
  • Biljana Nikolić
    • 1
  • Nataša Simin
    • 2
  • Nebojša Jasnić
    • 1
  • Dragana Četojević-Simin
    • 3
  • Maja Krstić
    • 4
  • Jelena Knežević-Vukčević
    • 1
  1. 1.Faculty of BiologyUniversity of BelgradeBelgradeSerbia
  2. 2.Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental ProtectionUniversity of Novi SadNovi SadSerbia
  3. 3.Faculty of Medicine, Oncology Institute of VojvodinaUniversity of Novi SadSremska KamenicaSerbia
  4. 4.Faculty of ChemistryUniversity of BelgradeBelgradeSerbia

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