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Plant Foods for Human Nutrition

, Volume 71, Issue 1, pp 28–34 | Cite as

Effect of Allium flavum L. and Allium melanantherum Panč. Extracts on Oxidative DNA Damage and Antioxidative Enzymes Superoxide Dismutase and Catalase

  • Dragana Mitić-Ćulafić
  • Biljana Nikolić
  • Nataša Simin
  • Nebojša Jasnić
  • Dragana Četojević-Simin
  • Maja Krstić
  • Jelena Knežević-Vukčević
Original Paper

Abstract

Allium flavum L. and Allium melanantherum Panč. are wild growing plants used in traditional diet in Balkan region. While chemical composition and some biological activities of A. flavum have been reported, A. melanantherum, as an endemic in the Balkan Peninsula, has never been comprehensively examined. After chemical characterization of A. melanantherum, we examined the protective effect of methanol extracts of both species against t-butyl hydro-peroxide (t-BOOH)-induced DNA damage and mutagenesis. The bacterial reverse mutation assay was performed on Escherichia coli WP2 oxyR strain. DNA damage was monitored in human fetal lung fibroblasts (MRC-5) with alkaline comet assay. Obtained results indicated that extracts reduced t-BOOH-induced DNA damage up to 70 and 72 % for A. flavum and A. melanantherum extract, respectively, and showed no effect on t-BOOH-induced mutagenesis. Since the results indicated modulatory effect on cell-mediated antioxidative defense, the effect of extracts on total protein content, and superoxide dismutase (SOD) and catalase (CAT) amounts and activities were monitored. Both extracts increased total protein content, while the increase of enzyme amount and activity was obtained only with A. melanantherum extract and restricted to CAT. The activity of CuZnSOD family was not affected, while SOD1 and SOD2 amounts were significantly decreased, indicating potential involvement of extracellular CuZnSOD. Obtained results strongly support the traditional use of A. flavum and A. melanantherum in nutrition and recommend them for further study.

Keywords

Allium flavum Allium melanantherum Antigenotoxicity Oxidative DNA damage Antioxidative enzymes 

Abbreviations

CAT

catalase

ROS

reactive oxygen species

SOD

superoxide dismutase

t-BOOH

t-butyl hydroperoxide

Notes

Acknowledgments

This work was supported by the Ministry of Science of Republic of Serbia, Project No. 172058.

Compliance with Ethical Standards

Conflict of Interest

The authors declare no conflict of interest.

Supplementary material

11130_2015_519_MOESM1_ESM.doc (98 kb)
Supplementary Table 1 (DOC 98 kb)
11130_2015_519_MOESM2_ESM.doc (28 kb)
Supplementary Table 2 (DOC 27 kb)
11130_2015_519_MOESM3_ESM.doc (28 kb)
Supplementary Table 3 (DOC 28 kb)

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Dragana Mitić-Ćulafić
    • 1
  • Biljana Nikolić
    • 1
  • Nataša Simin
    • 2
  • Nebojša Jasnić
    • 1
  • Dragana Četojević-Simin
    • 3
  • Maja Krstić
    • 4
  • Jelena Knežević-Vukčević
    • 1
  1. 1.Faculty of BiologyUniversity of BelgradeBelgradeSerbia
  2. 2.Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental ProtectionUniversity of Novi SadNovi SadSerbia
  3. 3.Faculty of Medicine, Oncology Institute of VojvodinaUniversity of Novi SadSremska KamenicaSerbia
  4. 4.Faculty of ChemistryUniversity of BelgradeBelgradeSerbia

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