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Relationship Between Metabolites Composition and Biological Activities of Phyllanthus niruri Extracts Prepared by Different Drying Methods and Solvents Extraction

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Abstract

The study investigated the changes in the metabolite, antioxidant and α-glucosidase inhibitory activities of Phyllanthus niruri after three drying treatments: air, freeze and oven dryings. Water extracts and extracts obtained using different solvent ratios of ethanol and methanol (50, 70, 80 and 100 %) were compared. The relationships among the antioxidant, α-glucosidase inhibitory activity and metabolite levels of the extracts were evaluated using partial least-square analysis (PLS). The solvent selectivity was assessed based on the phytochemical constituents present in the extract and their concentrations quantitatively analyzed using high performance liquid chromatography. The freeze-dried P. niruri samples that were extracted with the mixture of ethanol or methanol with low ratio of water showed higher biological activity values compared with the other extracts. The PLS results for the ethanolic with different ratio and water extracts demonstrated that phenolic acids (chlorogenic acid and ellagic acid) and flavonoids were highly linked to strong α-glucosidase inhibitory and antioxidant activities.

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Abbreviations

PLS:

Partial least-squares analysis

HPLC:

High performance liquid chromatography

AD:

Air drying

FD:

Freeze drying

OD:

Oven drying

TPC:

Total phenolic content

DPPH:

Diphenylpicrylhydrazyl

IC50 :

Half maximal inhibitory concentration

FRAP:

Ferric reducing antioxidant potential

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Acknowledgments

The authors wish to thank Universiti Putra Malaysia and Ministry of Agriculture (MOA) for the NRGS grant provided (NH0612D008).

Conflict of interest

The authors declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects.

Author information

Authors and Affiliations

  1. Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Ahmed Mediani & Faridah Abas

  2. Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Faridah Abas, Intan Safinar Ismail & Khozirah Shaari

  3. Faculty of Pharmacy, International Islamic University Malaysia, Kuantan, 25200, Pahang, Malaysia

    Alfi Khatib

  4. Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Chin Ping Tan

  5. Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Intan Safinar Ismail & Khozirah Shaari

  6. Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia

    Amin Ismail

  7. Al-Moalim BinLaden Chair for Scientific Miracles of Prophetic Medicine, Scientific Chairs Unit, Taibah University, P.O. Box 30001, Madinah al Munawarah, 41311, Saudi Arabia

    N. H. Lajis

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  1. Ahmed Mediani
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  2. Faridah Abas
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Correspondence to Faridah Abas.

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Mediani, A., Abas, F., Khatib, A. et al. Relationship Between Metabolites Composition and Biological Activities of Phyllanthus niruri Extracts Prepared by Different Drying Methods and Solvents Extraction. Plant Foods Hum Nutr 70, 184–192 (2015). https://doi.org/10.1007/s11130-015-0478-5

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  • DOI: https://doi.org/10.1007/s11130-015-0478-5

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