Plant Foods for Human Nutrition

, Volume 70, Issue 2, pp 184–192 | Cite as

Relationship Between Metabolites Composition and Biological Activities of Phyllanthus niruri Extracts Prepared by Different Drying Methods and Solvents Extraction

  • Ahmed Mediani
  • Faridah AbasEmail author
  • Alfi Khatib
  • Chin Ping Tan
  • Intan Safinar Ismail
  • Khozirah Shaari
  • Amin Ismail
  • N. H. Lajis
Original Paper


The study investigated the changes in the metabolite, antioxidant and α-glucosidase inhibitory activities of Phyllanthus niruri after three drying treatments: air, freeze and oven dryings. Water extracts and extracts obtained using different solvent ratios of ethanol and methanol (50, 70, 80 and 100 %) were compared. The relationships among the antioxidant, α-glucosidase inhibitory activity and metabolite levels of the extracts were evaluated using partial least-square analysis (PLS). The solvent selectivity was assessed based on the phytochemical constituents present in the extract and their concentrations quantitatively analyzed using high performance liquid chromatography. The freeze-dried P. niruri samples that were extracted with the mixture of ethanol or methanol with low ratio of water showed higher biological activity values compared with the other extracts. The PLS results for the ethanolic with different ratio and water extracts demonstrated that phenolic acids (chlorogenic acid and ellagic acid) and flavonoids were highly linked to strong α-glucosidase inhibitory and antioxidant activities.


α-Glucosidase inhibitory activity Phyllanthus niruri PLS TPC Antioxidant activity 



Partial least-squares analysis


High performance liquid chromatography


Air drying


Freeze drying


Oven drying


Total phenolic content




Half maximal inhibitory concentration


Ferric reducing antioxidant potential



The authors wish to thank Universiti Putra Malaysia and Ministry of Agriculture (MOA) for the NRGS grant provided (NH0612D008).

Conflict of interest

The authors declare that they have no conflict of interest. This article does not contain any studies with human or animal subjects.

Supplementary material

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ESM 1 (DOC 43 kb)
11130_2015_478_MOESM2_ESM.doc (212 kb)
ESM 2 (DOC 212 kb)
11130_2015_478_MOESM3_ESM.doc (46 kb)
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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Ahmed Mediani
    • 1
  • Faridah Abas
    • 1
    • 2
    Email author
  • Alfi Khatib
    • 3
  • Chin Ping Tan
    • 4
  • Intan Safinar Ismail
    • 2
    • 5
  • Khozirah Shaari
    • 2
    • 5
  • Amin Ismail
    • 6
  • N. H. Lajis
    • 7
  1. 1.Department of Food Science, Faculty of Food Science and TechnologyUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Laboratory of Natural Products, Institute of BioscienceUniversiti Putra MalaysiaSerdangMalaysia
  3. 3.Faculty of PharmacyInternational Islamic University MalaysiaKuantanMalaysia
  4. 4.Department of Food Technology, Faculty of Food Science and TechnologyUniversiti Putra MalaysiaSerdangMalaysia
  5. 5.Department of Chemistry, Faculty of ScienceUniversiti Putra MalaysiaSerdangMalaysia
  6. 6.Department of Nutrition and Dietetics, Faculty of Medicine and Health SciencesUniversiti Putra MalaysiaSerdangMalaysia
  7. 7.Al-Moalim BinLaden Chair for Scientific Miracles of Prophetic Medicine, Scientific Chairs UnitTaibah UniversityMadinah al MunawarahSaudi Arabia

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