Plant Foods for Human Nutrition

, Volume 70, Issue 2, pp 119–127 | Cite as

Influence of Extraction Methods on the Yield of Steviol Glycosides and Antioxidants in Stevia rebaudiana Extracts

  • Angela Periche
  • Maria Luisa Castelló
  • Ana Heredia
  • Isabel Escriche
Original Paper


This study evaluated the application of ultrasound techniques and microwave energy, compared to conventional extraction methods (high temperatures at atmospheric pressure), for the solid–liquid extraction of steviol glycosides (sweeteners) and antioxidants (total phenols, flavonoids and antioxidant capacity) from dehydrated Stevia leaves. Different temperatures (from 50 to 100 °C), times (from 1 to 40 min) and microwave powers (1.98 and 3.30 W/g extract) were used. There was a great difference in the resulting yields according to the treatments applied. Steviol glycosides and antioxidants were negatively correlated; therefore, there is no single treatment suitable for obtaining the highest yield in both groups of compounds simultaneously. The greatest yield of steviol glycosides was obtained with microwave energy (3.30 W/g extract, 2 min), whereas, the conventional method (90 °C, 1 min) was the most suitable for antioxidant extraction. Consequently, the best process depends on the subsequent use (sweetener or antioxidant) of the aqueous extract of Stevia leaves.


Microwave energy Ultrasound technique Antioxidants Phenols Flavonoids Steviol glycosides 



The authors thank the Universitat Politècnica de València for funding the project PAID 2011-ref: 2012 and the Generalitat Valenciana (Spain) for the project GV/2013/029.

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

11130_2015_475_MOESM1_ESM.doc (46 kb)
ESM 1(DOC 46 kb)


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Angela Periche
    • 1
  • Maria Luisa Castelló
    • 1
  • Ana Heredia
    • 1
  • Isabel Escriche
    • 1
  1. 1.Institute of Food Engineering for Development (IUIAD). Food Technology Department (DTA)Universitat Politècnica de ValènciaValenciaSpain

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