Plant Foods for Human Nutrition

, Volume 69, Issue 3, pp 203–208 | Cite as

Development of an Iron-enriched High-yieldings Indica Rice Cultivar by Introgression of A High-iron Trait from Transgenic Iron-biofortified Rice

  • Soumitra Paul
  • Nusrat Ali
  • Swapan K Datta
  • Karabi DattaEmail author
Original Paper


Low level of iron in staple food crops is one reason for the predominance of iron-deficiency anemia in developing countries. Most of the iron in rice grains accumulates in the outer aleurone layer and embryo, which are removed during milling, and the edible endosperm contains very low amounts of iron. In an effort to increase iron nutrition, we report here the transgene introgression of a high-iron trait into a high-yielding indica rice cultivar. The ferritin gene from soybean (soyfer1) was introduced into rice plants through interbreeding between soybean ferritin-overexpressing transgenic IR68144 and the high-yielding cultivar Swarna. The stable integration of the soyfer1 gene was confirmed in the BC2F4 generation, and the hybrid seeds showed 2.6-fold soybean ferritin gene expression over the recurrent parent Swarna. The hybrid milled seeds revealed a 2.54-fold increase in iron and 1.54-fold increase in zinc compared to Swarna. Agronomic data and an SSR marker analysis of the hybrid rice plants were taken into account for NIL character identification.


Introgression Ferritin Overexpression Iron Rice Near-isogenic lines 



Iron-deficiency Anemia


Dry Weight


Near-isogenic Line


Polymerase Chain Reaction


Polymorphism Information Content


Quantitative Trait Locus


Recombinant Inbred Lines


Simple Sequence Repeat



We extend our special thanks to the Department of Biotechnology (DBT) and University Grants Commission (UGC), Government of India for financial assistance. We express our sincere gratitude to Dr. Rajeev Varshney for his valuable guidance and kind cooperation in the PIC data analysis of the SSR marker studies.

Conflict of Interest

Each author of the article does not have any conflict of interest. The article does not contain any studies with human or animal subjects.

Supplementary material

11130_2014_431_MOESM1_ESM.pdf (22 kb)
ESM 1 The figure showing the presence of soyfer1 gene in the progeny of FR 19-7 and iron and zinc contents of transgenic seeds of FR-19-7-6 (PDF 21 kb)
11130_2014_431_MOESM2_ESM.pdf (30 kb)
ESM 2 The figure showing iron and zinc content in seeds of local cultivars (PDF 29 kb)
11130_2014_431_MOESM3_ESM.pdf (29 kb)
ESM 3 The figure showing developmental pattern of BC2F4 generations (PDF 29 kb)
11130_2014_431_MOESM4_ESM.pdf (17 kb)
ESM 4 The figure showing dendrogram analysis of hybridized plants (PDF 16 kb)
11130_2014_431_MOESM5_ESM.pdf (23 kb)
ESM 5 Table represents the morphological characters of hybridized plants (PDF 22 kb)
11130_2014_431_MOESM6_ESM.pdf (9 kb)
ESM 6 Table represents the morphological characters of hybrid seeds (PDF 9 kb)


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Soumitra Paul
    • 1
  • Nusrat Ali
    • 1
  • Swapan K Datta
    • 1
    • 2
  • Karabi Datta
    • 1
    Email author
  1. 1.Plant Molecular Biology and Biotechnology Laboratory, Department of BotanyUniversity of CalcuttaKolkataIndia
  2. 2.Division of Crop Science, Indian Council of Agricultural Research (ICAR)New DelhiIndia

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