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Plant Foods for Human Nutrition

, Volume 68, Issue 3, pp 293–298 | Cite as

Extracts from Black Carrot Tissue Culture as Potent Anticancer Agents

  • Canan Sevimli-Gur
  • Burcu Cetin
  • Seref Akay
  • Sultan Gulce-Iz
  • Ozlem Yesil-CeliktasEmail author
Original Paper

Abstract

Black carrots contain anthocyanins possessing enhanced physiological activities. Explants of young black carrot shoots were cultured in Murashige and Skoog (MS) medium for callus initiation and were transferred to new MS medium supplemented with four different combinations of 2,4-dichlorophenoxyacetic acid and kinetin. Subsequently, the lyophilized calli and black carrot harvested from fields were subjected to ultrasound extraction with ethanol at a ratio of 1:15 (w:v). Obtained extracts were applied to various human cancer cell lines including MCF-7 SK-BR-3 and MDA-MB-231 (human breast adenocarcinomas), HT-29 (human colon adenocarcinoma), PC-3 (human prostate adenocarcinoma), Neuro 2A (Musmusculus neuroblastoma) cancer cell lines and VERO (African green monkey kidney) normal cell line by MTT assay. The highest cytotoxic activity was achieved against Neuro-2A cell lines exhibiting viability of 38–46 % at 6.25 μg/ml concentration for all calli and natural extracts. However, a significantly high IC50 value of 170.13 μg/ml was attained in normal cell line VERO indicating that its natural counterpart is an ideal candidate for treatment of brain cancer without causing negative effects to normal healthy cells.

Keywords

Black carrot Plant cell culture Callus Anticarcinogen Cytotoxicity Antioxidant 

Notes

Acknowledgements

Access to the facilities of Animal Cell Culture and Supercritical Fluid Technology Laboratories of Bioengineering Department at Ege University is highly appreciated.

Conflict of Interest

Authors declare not to have any conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Canan Sevimli-Gur
    • 1
  • Burcu Cetin
    • 2
  • Seref Akay
    • 3
  • Sultan Gulce-Iz
    • 3
  • Ozlem Yesil-Celiktas
    • 3
    Email author
  1. 1.Department of Biology, Science and Art FacultyKocaeli UniversityKocaeliTurkey
  2. 2.Department of Biology, Science and Art FacultyDumlupinar UniversityKutahyaTurkey
  3. 3.Department of Bioengineering, Faculty of EngineeringEge UniversityBornovaTurkey

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