Plant Foods for Human Nutrition

, Volume 67, Issue 4, pp 384–392

Chemical Composition and Anti-inflammatory Properties of the Unsaponifiable Fraction from Awara (Astrocaryum vulgare M.) Pulp Oil in Activated J774 Macrophages and in a Mice Model of Endotoxic Shock

  • Emilie Bony
  • Frédéric Boudard
  • Emilie Dussossoy
  • Karine Portet
  • Pierre Brat
  • Jean Giaimis
  • Alain Michel
Original Paper

Abstract

Awara (Astrocaryum vulgare M.) pulp oil has been shown to possess anti-inflammatory properties in vivo, and contains an unsaponifiable matter rich in bioactive compounds. This study focused on the ethanolic unsaponifiable fraction (EUF) of awara pulp oil. Its chemical composition has been characterized: carotenoid, phytosterol, and tocopherol contents represent 125.7, 152.6, and 6.8 μg/mg of EUF, respectively. We further evaluated this fraction for anti-inflammatory properties in J774 macrophages activated by lipopolysaccharide (LPS) plus interferon (IFN) γ to understand the biological effects of awara pulp oil. EUF strongly decreased nitric oxide (NO), prostaglandin E2, tumour necrosis factor (TNF) α, and interleukin (IL) -6 and -10 production in activated J774 cells. Moreover, it inhibited expression of inducible NO synthase and cyclooxygenases-2 in vitro. The anti-inflammatory properties of EUF were also confirmed in vivo by modulation of TNFα, IL-6 and IL-10 serum concentration in an endotoxic shock model. Pre-treatment with awara oil fraction offers promise as a protective means to lower the production of excessive amounts of pro-inflammatory molecules.

Keywords

Awara (Astrocaryum vulgare M.) Unsaponifiable fraction Carotenoids Phytosterols Tocopherols Anti-inflammatory J774 macrophages Endotoxic shock 

Abbreviations

COX

Cyclooxygenases

EUF

Ethanolic unsaponifiable fraction

IFN

Interferon

IL

Interleukin

LPS

Lipopolysaccharide

NOS

Nitric oxide synthase

ORAC

Oxygen radical absorbance capacity

PG

Prostaglandin

ROS

Reactive oxygen species

SNP

Sodium nitroprusside

TNF

Tumour necrosis factor

Supplementary material

11130_2012_323_MOESM1_ESM.doc (160 kb)
ESM 1(DOC 159 kb)

References

  1. 1.
    Kahn F (2008) The genus Astrocaryum (Arecaceae). Rev Peru Biol 15:031–048Google Scholar
  2. 2.
    Bony E, Boudard F, Brat P, Dussossoy E, Portet K, Giaimis J, Michel A (2012) Awara (Astrocaryum vulgare M.) pulp oil: chemical characterization, and anti-inflammatory properties in a mice model of endotoxic shock and a rat model of pulmonary inflammation. Fitoterapia 83:33–43CrossRefGoogle Scholar
  3. 3.
    Ryan E, Galvin K, O’Connor TP, Maguire AR, O’Brien NM (2007) Phytosterol, squalene, tocopherol content and fatty acid profile of selected seeds, grains, and legumes. Plant Foods Hum Nutr 62:85–91CrossRefGoogle Scholar
  4. 4.
    Bester D, Esterhuyse AJ, Truter EJ, van Rooyen J (2010) Cardiovascular effects of edible oils: a comparison between four popular edible oils. Nutr Res Rev 23:334–348CrossRefGoogle Scholar
  5. 5.
    Moreno J (2003) Effect of olive oil minor components on oxidative stress and arachidonic acid mobilization and metabolism by macrophages RAW 264.7. Free Radic Biol Med 35:1073–1081CrossRefGoogle Scholar
  6. 6.
    Vivancos M, Moreno J (2008) Effect of resveratrol, tyrosol and β-sitosterol on oxidized low-density lipoprotein-stimulated oxidative stress, arachidonic acid release and prostaglandin E2 synthesis by RAW 264.7 macrophages. Br J Nutr 99:1199–1207Google Scholar
  7. 7.
    Edem DO (2002) Palm oil: Biochemical, physiological, nutritional, hematological, and toxicological aspects: a review. Plant Foods Hum Nutr 57:319–341Google Scholar
  8. 8.
    Federico A, Morgillo F, Tuccillo C, Ciardiello F, Loguercio C (2007) Chronic inflammation and oxidative stress in human carcinogenesis. Int J Cancer 121:2381–2386CrossRefGoogle Scholar
  9. 9.
    Halayko A (2003) Mechanisms of inflammation-mediated airway smooth muscle plasticity and airways remodeling in asthma. Respir Physiol Neurobiol 137:209–222CrossRefGoogle Scholar
  10. 10.
    Calder PC, Albers R, Antoine JM, Blum S, Bourdet-Sicard R, Ferns GA, Folkerts G, Friedmann PS, Frost GS, Guarner F, Løvik M, Macfarlane S, Meyer PD, M’Rabet L, Serafini M, van Eden W, van Loo J, Vas Dias W, Vidry S, Winklhofer-Roob BM, Zhao J (2009) Inflammatory disease processes and interactions with nutrition. Br J Nutr 101:1–45Google Scholar
  11. 11.
    Coleman JW (2001) Nitric oxide in immunity and inflammation. Int Immunopharmacol 1:1397–1406CrossRefGoogle Scholar
  12. 12.
    Simon LS (1999) Role and regulation of cyclooxygenase-2 during inflammation. Am J Med 106:37S–42SCrossRefGoogle Scholar
  13. 13.
    Medzhitov R (2008) Origin and physiological roles of inflammation. Nature 454:428–435CrossRefGoogle Scholar
  14. 14.
    Dussossoy E, Brat P, Bony E, Boudard F, Poucheret P, Mertz C, Giaimis J, Michel A (2011) Characterization, anti-oxidative and anti-inflammatory effects of Costa Rican noni juice (Morinda citrifolia L.). J Ethnopharmacol 133:108–115CrossRefGoogle Scholar
  15. 15.
    Boudard F, Vallot N, Cabaner C, Bastide M (1994) Chemiluminescence and nitrite determinations by the MALU macrophage cell line. J Immunol Methods 174:259–268CrossRefGoogle Scholar
  16. 16.
    Krinsky N, Johnson E (2005) Carotenoid actions and their relation to health and disease. Mol Aspects Med 26:459–516CrossRefGoogle Scholar
  17. 17.
    Navarro A, De Las HB, Villar A (2001) Anti-inflammatory and immunomodulating properties of a sterol fraction from Sideritis foetens CLEM. Biol Pharm Bull 24:470–473CrossRefGoogle Scholar
  18. 18.
    Akihisa T, Yasukawa K, Yamaura M, Ukiya M, Kimura Y, Shimizu N, Arai K (2000) Triterpene alcohol and sterol ferulates from rice bran and their anti-inflammatory effects. J Agric Food Chem 48:2313–2319CrossRefGoogle Scholar
  19. 19.
    Nashed B, Yeganeh B, HayGlass KT, Moghadasian MH (2005) Antiatherogenic effects of dietary plant sterols are associated with inhibition of proinflammatory cytokine production in Apo E-KO mice. J Nutr 135:2438–2444Google Scholar
  20. 20.
    Reiter E, Jiang Q, Christen S (2007) Anti-inflammatory properties of α- and γ-tocopherol. Mol Aspects Med 28:668–691CrossRefGoogle Scholar
  21. 21.
    Bai SK, Lee SJ, Na HJ, Ha KS, Han JA, Lee H, Kwon YG, Chung CK, Kim YM (2005) β-Carotene inhibits inflammatory gene expression in lipopolysaccharide-stimulated macrophages by suppressing redox-based NF-κB activation. Exp Mol Med 37:323–334Google Scholar
  22. 22.
    Fuller B, Smith D, Howerton A, Kern D (2006) Anti-inflammatory effects of CoQ10 and colorless carotenoids. J Cosmet Dermatol 5:30–38CrossRefGoogle Scholar
  23. 23.
    Jiang Q, Elson-Schwab I, Bedord C, Ames B (2000) γ-Tocopherol and its major metabolite, in contrast to α-tocopherol, inhibit cyclooxygenase activity in macrophages and epithelial cells. Proc Natl Acad Sci 97:11494–11499CrossRefGoogle Scholar
  24. 24.
    Devaraj S, Jialal I (2005) Alpha-tocopherol decreases tumor necrosis factor-alpha mRNA and protein from activated human monocytes by inhibition of 5-lipoxygenase. Free Radic Biol Med 38:1212–1220CrossRefGoogle Scholar
  25. 25.
    Brasier AR (2006) The NF-kappaB regulatory network. Cardiovasc Toxicol 6:111–130CrossRefGoogle Scholar
  26. 26.
    Islam MS, Yoshida H, Matsuki N, Ono K, Nagasaka R, Ushio H, Guo Y, Hiramatsu T, Hosoya T, Murata T, Hori M, Ozaki H (2009) Antioxidant, free radical-scavenging, and NF-kB-inhibitory activties of phytosteryl ferulates: structure-activity studies. J Pharmacol Sci 111:328–337CrossRefGoogle Scholar
  27. 27.
    Rimbach G, Minihane AM, Majewicz J, Fischer A, Pallauf J, Virgli F, Weinberg PD (2002) Regulation of cell signalling by vitamin E. Proc Nutr Soc 61:415–425CrossRefGoogle Scholar
  28. 28.
    Annane D, Bellissant E, Cavaillon JM (2005) Septic shock. Lancet 365:63–78CrossRefGoogle Scholar
  29. 29.
    Novoselova E, Lunin SM, Novoselova TV, Khrenov MO, Glushkova OV, Avkhacheva NV, Safronova VG, Fesenko EE (2009) Naturally occurring antioxidant nutrients reduce inflammatory response in mice. Eur J Pharmacol 615:234–240CrossRefGoogle Scholar
  30. 30.
    Galley HF, Howdle PD, Walker BE, Webster NR (1997) The effects of intravenous antioxidants in patients with septic shock. Free Radic Biol Med 23:768–774CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Emilie Bony
    • 1
    • 3
  • Frédéric Boudard
    • 2
  • Emilie Dussossoy
    • 1
  • Karine Portet
    • 1
  • Pierre Brat
    • 3
  • Jean Giaimis
    • 2
  • Alain Michel
    • 1
  1. 1.Laboratoire de Pharmacologie et Physiopathologie Expérimentales, UMR Qualisud, Faculté de PharmacieUniversité Montpellier IMontpellier Cedex 5France
  2. 2.Laboratoire d’Immunologie, Faculté de PharmacieUniversité Montpellier IMontpellier Cedex 5France
  3. 3.Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Département PERSYSTUMR QualisudMontpellier Cedex 5France

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