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Plant Foods for Human Nutrition

, Volume 67, Issue 1, pp 71–75 | Cite as

Total Polyphenols, Antioxidant and Antiproliferative Activities of Different Extracts in Mungbean Seeds and Sprouts

  • Dong-Kwan Kim
  • Seok Cheol Jeong
  • Shela Gorinstein
  • Sang-Uk Chon
Original Paper

Abstract

The aim of this investigation was to evaluate the biological, alcohol dehydrogenase (ADH) and antiproliferative activities of different extracts of mungbean seeds and sprouts. All extracts from the sprouts showed higher contents of total phenolics (TP), total flavonoids (TF), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than from seeds. The highest DPPH and tyrosinase inhibition activities were registered in ethyl acetate (EtOAc) extract. ADH activity of methanol (MeOH), n-hexane (n-hexane) and n-butanol (n-BuOH) extracts from sprouts was significantly higher (P < 0.05) than from seeds. However, the highest ADH activity was found in water extract of seeds. According to 3-(4, 5-dimethylthiazol -2-yl)-2, 5-diphenyltetrazolium bromide) (MTT) assay, extracts from sprouts were more effective against Calu-6 (human pulmonary carcinoma) and SNU-601 (human gastric carcinoma) cells than from seeds. EtOAc extract showed the highest antiproliferative activity in both sprouts and seeds, followed by n-hexane extraction. During sprouting of mungbean, all the studied components significantly increased. In conclusion, the extracts of sprouts are more effective than from seeds and could be a potential source of antioxidants linked with health benefits.

Keywords

Mungbean seeds Sprouts Total phenolics Total flavonoids Antioxidant and antiproliferative activities 

Abbreviations

ADH

Alcohol dehydrogenase

Calu-6

Human pulmonary carcinoma cell line

DPPH

1, 1-diphenyl-2-picrylhydrazyl radical

MTT

3-(4, 5-dimethylthiazol -2-yl)-2, 5-diphenyltetrazolium bromide

SNU-601

Human gastric carcinoma cell line

Notes

Acknowledgment

Special thanks to Mrs. Judy Siegel-Itzkovich, The Jerusalem Post’s Health & Science Reporter, for her help in improving the English style of the manuscript.

References

  1. 1.
    Thomas RMJ, Fukaic S, Peoples MB (2004) The effect of timing and severity of water deficit on growth, development, yield accumulation and nitrogen fixation of mungbean. Field Crop Res 86:67–80CrossRefGoogle Scholar
  2. 2.
    Choi Y, Jeong H-S, Lee J (2007) Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem 103:130–138CrossRefGoogle Scholar
  3. 3.
    Samotyja U, Zdzieblowski T, Szlachta M, Malecka M (2007) Antioxidant properties of extracts from plant germs. Zywnosc 14:122–128Google Scholar
  4. 4.
    Cevallos-Casals BA, Cisneros-Zevallos L (2010) Impact of germination on phenolic content and antioxidant activity of 13 edible seed species. Food Chem 119:1485–1490CrossRefGoogle Scholar
  5. 5.
    Cao D, Li H, Yi J, Zhang J, Che H, Cao J, Yang L, Zhu Ch, Jiang W (2011) Antioxidant properties of the mungbean flavonoids on alleviating heat stress. PLoS One 6:e21070CrossRefGoogle Scholar
  6. 6.
    Anwar F, Latif S, Przybylski R, Sultana B, Ashraf M (2007) Chemical composition and antioxidant activity of seeds of different cultivars of mungbean. J Food Sci 72:S503–S510CrossRefGoogle Scholar
  7. 7.
    Pasko P, Sajewicz M, Gorinstein S, Zachwieja Z (2008) Analysis of the selected phenolic acids and flavonoids in Amaranthus cruentus and Chenopodium quinoa seeds and sprouts by HPLC method. Acta Chromatographica 20:661–672CrossRefGoogle Scholar
  8. 8.
    Liu B, Guo X, Zhu K, Liu Y (2011) Nutritional evaluation and antioxidant activity of sesame sprouts. Food Chem 129:799–803CrossRefGoogle Scholar
  9. 9.
    Heo BGu, Chon SU, Park YJ, Bae JH, Park SM, Park YS, Jang HG, Gorinstein S (2009) Antiproliferative activity of Korean wild vegetables on different human tumor cell lines. Plant Foods Hum Nutr 64:257–263CrossRefGoogle Scholar
  10. 10.
    Chon SU, Heo BG, Park YS, Kim DK, Gorinstein S (2009) Total phenolics level, antioxidant activities and cytotoxicity of young sprouts of some traditional Korean salad plants. Plant Foods Hum Nutr 64:25–31CrossRefGoogle Scholar
  11. 11.
    Russo GL (2007) Ins and outs of dietary phytochemicals in cancer chemoprevention. Biochem Pharm 74:533–544CrossRefGoogle Scholar
  12. 12.
    Estomba D, Ladio A, Lozada M (2006) Medicinal wild plant knowledge and gathering patterns in a Mapuche community from North-western Patagonia. J Ethnopharmacol 103:109–119CrossRefGoogle Scholar
  13. 13.
    Manosroi J, Dhumtanom P, Manosroi A (2006) Anti-proliferative activity of essential oil extracted from Thai medicinal plants on KB and P338 cell lines. Cancer Lett 235:114–120CrossRefGoogle Scholar
  14. 14.
    Wongekalak L, Sakulsom P, Jirasripongpun K, Hongsprabhas P (2011) Potential use of antioxidative mungbean protein hydrolysate as an anticancer asiatic acid carrier. Food Res Int 44:812–817CrossRefGoogle Scholar
  15. 15.
    Singleton VL, Rossi JA (1965) A colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 16:144–158Google Scholar
  16. 16.
    Bao JS, Cai Y, Sun M, Wang GY, Corke H (2005) Anthocyanins, flavonoids, and free radical scavenging activity of Chinese bayberry (Myrica rubra) extracts and their color properties and stability. J Agric Food Chem 53:2327–2332CrossRefGoogle Scholar
  17. 17.
    Blosi MS (1958) Antioxidant determinations by use of a stable free radical. Nature 26:1199–1200CrossRefGoogle Scholar
  18. 18.
    Chen QX, Kubo I (2002) Kinetics of mushroom tyrosinase inhibition by quercetin. J Agric Food Chem 50:4108–4112CrossRefGoogle Scholar
  19. 19.
    Lebsack ME, Petersen DR, Collins AC (1977) Preferential inhibition of the low Km aldehyde dehydrogenase activity by pargyline. Biochemical Pharmacol 26:1151–1154CrossRefGoogle Scholar
  20. 20.
    SAS (Statistical Analysis Systems) Institute. 2000. SAS/STAT user’s guide. Version 7. Electronic Version. Cary, NCGoogle Scholar
  21. 21.
    Jastrzębski Z, Tashma Z, Katrich E, Gorinstein S (2007) Biochemical characteristics of the herb mixture Prolipid as a plant food supplement and medicinal remedy. Plant Foods Hum Nutr 62:145–150CrossRefGoogle Scholar
  22. 22.
    Jung MJ, Heo SI, Wang MH (2008) Free radical scavenging and total phenolic contents from methanolic extracts of Ulmus davidiana. Food Chem 108:482–487CrossRefGoogle Scholar
  23. 23.
    Velioglu YS, Mazza G, Gao L, Oomah BD (1998) Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 46:4113–4117CrossRefGoogle Scholar
  24. 24.
    Zhou K, Yu L (2006) Total phenolic contents and antioxidant properties of commonly consumed vegetables grown in Colorado. LWT - Food Sci Technol 39:1155–1162CrossRefGoogle Scholar
  25. 25.
    Vellingiri V, Nandety A, Biesalski HK (2011) Antioxidant potential and health relevant functionality of traditionally processed Cassia hirsuta L. seeds: An Indian underutilized food legume. Plant Foods Hum Nutr 66:245–253CrossRefGoogle Scholar
  26. 26.
    Lee K-G, Shibamoto T (2000) Antioxidant properties of aroma compounds isolated from soybeans and mung Beans. J Agric Food Chem 48:4290–4293CrossRefGoogle Scholar
  27. 27.
    McCue P, Kalidas S (2002) A biochemical analysis of mungbean (Vigna radiata) response to microbial polysaccharides and potential phenolic-enhancing effects for nutraceutical applications. Food Biotechnol 16:57–79CrossRefGoogle Scholar
  28. 28.
    Kittipongpatana OS, Burapadaja S, Kittipongpatana N (2009) Carboxymethyl mungbean starch as a new pharmaceutical gelling agent for topical preparation. Drug Dev Ind Pharm 35:34–42CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, Inc. 2012

Authors and Affiliations

  • Dong-Kwan Kim
    • 1
  • Seok Cheol Jeong
    • 2
  • Shela Gorinstein
    • 3
  • Sang-Uk Chon
    • 4
  1. 1.Jeonnam Agricultural Research and Extension ServicesNajuSouth Korea
  2. 2.Department of Life ScienceSunchon National UniversitySuncheonSouth Korea
  3. 3.Institute for Drug Research, School of PharmacyThe Hebrew University—Hadassah Medical SchoolJerusalemIsrael
  4. 4.EFARINET Co. Ltd., BI CenterChosun UniversityGwangjuSouth Korea

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