Plant Foods for Human Nutrition

, Volume 67, Issue 1, pp 71–75 | Cite as

Total Polyphenols, Antioxidant and Antiproliferative Activities of Different Extracts in Mungbean Seeds and Sprouts

  • Dong-Kwan Kim
  • Seok Cheol Jeong
  • Shela Gorinstein
  • Sang-Uk ChonEmail author
Original Paper


The aim of this investigation was to evaluate the biological, alcohol dehydrogenase (ADH) and antiproliferative activities of different extracts of mungbean seeds and sprouts. All extracts from the sprouts showed higher contents of total phenolics (TP), total flavonoids (TF), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than from seeds. The highest DPPH and tyrosinase inhibition activities were registered in ethyl acetate (EtOAc) extract. ADH activity of methanol (MeOH), n-hexane (n-hexane) and n-butanol (n-BuOH) extracts from sprouts was significantly higher (P < 0.05) than from seeds. However, the highest ADH activity was found in water extract of seeds. According to 3-(4, 5-dimethylthiazol -2-yl)-2, 5-diphenyltetrazolium bromide) (MTT) assay, extracts from sprouts were more effective against Calu-6 (human pulmonary carcinoma) and SNU-601 (human gastric carcinoma) cells than from seeds. EtOAc extract showed the highest antiproliferative activity in both sprouts and seeds, followed by n-hexane extraction. During sprouting of mungbean, all the studied components significantly increased. In conclusion, the extracts of sprouts are more effective than from seeds and could be a potential source of antioxidants linked with health benefits.


Mungbean seeds Sprouts Total phenolics Total flavonoids Antioxidant and antiproliferative activities 



Alcohol dehydrogenase


Human pulmonary carcinoma cell line


1, 1-diphenyl-2-picrylhydrazyl radical


3-(4, 5-dimethylthiazol -2-yl)-2, 5-diphenyltetrazolium bromide


Human gastric carcinoma cell line



Special thanks to Mrs. Judy Siegel-Itzkovich, The Jerusalem Post’s Health & Science Reporter, for her help in improving the English style of the manuscript.


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Copyright information

© Springer Science+Business Media, Inc. 2012

Authors and Affiliations

  • Dong-Kwan Kim
    • 1
  • Seok Cheol Jeong
    • 2
  • Shela Gorinstein
    • 3
  • Sang-Uk Chon
    • 4
    Email author
  1. 1.Jeonnam Agricultural Research and Extension ServicesNajuSouth Korea
  2. 2.Department of Life ScienceSunchon National UniversitySuncheonSouth Korea
  3. 3.Institute for Drug Research, School of PharmacyThe Hebrew University—Hadassah Medical SchoolJerusalemIsrael
  4. 4.EFARINET Co. Ltd., BI CenterChosun UniversityGwangjuSouth Korea

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