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Total Polyphenols, Antioxidant and Antiproliferative Activities of Different Extracts in Mungbean Seeds and Sprouts

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Abstract

The aim of this investigation was to evaluate the biological, alcohol dehydrogenase (ADH) and antiproliferative activities of different extracts of mungbean seeds and sprouts. All extracts from the sprouts showed higher contents of total phenolics (TP), total flavonoids (TF), and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity than from seeds. The highest DPPH and tyrosinase inhibition activities were registered in ethyl acetate (EtOAc) extract. ADH activity of methanol (MeOH), n-hexane (n-hexane) and n-butanol (n-BuOH) extracts from sprouts was significantly higher (P < 0.05) than from seeds. However, the highest ADH activity was found in water extract of seeds. According to 3-(4, 5-dimethylthiazol -2-yl)-2, 5-diphenyltetrazolium bromide) (MTT) assay, extracts from sprouts were more effective against Calu-6 (human pulmonary carcinoma) and SNU-601 (human gastric carcinoma) cells than from seeds. EtOAc extract showed the highest antiproliferative activity in both sprouts and seeds, followed by n-hexane extraction. During sprouting of mungbean, all the studied components significantly increased. In conclusion, the extracts of sprouts are more effective than from seeds and could be a potential source of antioxidants linked with health benefits.

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Abbreviations

ADH:

Alcohol dehydrogenase

Calu-6:

Human pulmonary carcinoma cell line

DPPH:

1, 1-diphenyl-2-picrylhydrazyl radical

MTT:

3-(4, 5-dimethylthiazol -2-yl)-2, 5-diphenyltetrazolium bromide

SNU-601:

Human gastric carcinoma cell line

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Acknowledgment

Special thanks to Mrs. Judy Siegel-Itzkovich, The Jerusalem Post’s Health & Science Reporter, for her help in improving the English style of the manuscript.

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Authors and Affiliations

  1. Jeonnam Agricultural Research and Extension Services, Naju, 520-715, South Korea

    Dong-Kwan Kim

  2. Department of Life Science, Sunchon National University, Suncheon, 540-742, South Korea

    Seok Cheol Jeong

  3. Institute for Drug Research, School of Pharmacy, The Hebrew University—Hadassah Medical School, Jerusalem, Israel

    Shela Gorinstein

  4. EFARINET Co. Ltd., BI Center, Chosun University, Gwangju, 501-759, South Korea

    Sang-Uk Chon

Authors
  1. Dong-Kwan Kim
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  2. Seok Cheol Jeong
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  3. Shela Gorinstein
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  4. Sang-Uk Chon
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Correspondence to Sang-Uk Chon.

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Kim, DK., Jeong, S.C., Gorinstein, S. et al. Total Polyphenols, Antioxidant and Antiproliferative Activities of Different Extracts in Mungbean Seeds and Sprouts. Plant Foods Hum Nutr 67, 71–75 (2012). https://doi.org/10.1007/s11130-011-0273-x

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  • DOI: https://doi.org/10.1007/s11130-011-0273-x

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