Ginsenoside Rp1 from Panax ginseng Exhibits Anti-cancer Activity by Down-regulation of the IGF-1R/Akt Pathway in Breast Cancer Cells

  • Ju-Hee Kang
  • Ki-Hoon Song
  • Jong-Kyu Woo
  • Myung Hwan Park
  • Man Hee Rhee
  • Changsun Choi
  • Seung Hyun Oh
ORIGINAL PAPER

Abstract

Cancer prevention is effective and reduces health care costs because cancer is often a preventable disease that can be affected by lifestyle factors. Therefore, researchers are interested in discovering natural compounds that have anticancer activities, such as delaying the development of cancer and preventing its progression. One such natural agent is ginseng (Panax ginseng), which is traditionally used in some parts of the world as a popular remedy for various diseases including cancer. We hypothesized that the ginsenoside Rp1, a component of ginseng, reduces cancer cell proliferation through inhibition of the insulin-like growth factor 1 receptor (IGF-1R)/Akt pathway. We first tested the efficacy of Rp1 against human breast cancer cell lines. Treatment with Rp1 inhibited breast cancer cell proliferation and inhibited both anchorage-dependent and -independent breast cancer cell colony formation. In addition, treatment with 20 μM Rp1 induced cycle arrest and apoptosis-mediated cell growth suppression. Our findings further indicated that Rp1 decreased the stability of the IGF-1R protein in breast cancer cells. Therefore, we suggest that Rp1 has potential as an anticancer drug and that IGF-1R is an important target for treatment and prevention of breast cancer.

Keywords

Akt Breast cancer Ginseng Ginsenoside Rp1 IGF-1R 

Abbreviations

DMEM

Dulbecco’s modified Eagle’s medium

FBS

fetal bovine serum

Hsp90

heat shock protein 90

IGF

insulin-like growth factor

IGF-1R

IGF-1 receptor

IL

interleukin

MMP

matrix metalloproteinase

MTT

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

NF

nuclear factor

PCR

polymerase chain reaction

PI3K

phosphoinositide 3-kinase

RT

reverse transcriptase

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Ju-Hee Kang
    • 1
    • 2
  • Ki-Hoon Song
    • 1
  • Jong-Kyu Woo
    • 3
  • Myung Hwan Park
    • 4
  • Man Hee Rhee
    • 5
  • Changsun Choi
    • 2
  • Seung Hyun Oh
    • 1
  1. 1.Division of Cancer BiologyNational Cancer CenterGoyang-siRepublic of Korea
  2. 2.Department of Food and Nutrition, College of Human EcologyChung-Ang UniversityAnsungRepublic of Korea
  3. 3.Seoul National University, College of MedicineSeoulRepublic of Korea
  4. 4.Ambo InstituteSeoulRepublic of Korea
  5. 5.College of Veterinary MedicineKyungpook National UniversityDaeguRepublic of Korea

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