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Plant Foods for Human Nutrition

, Volume 66, Issue 3, pp 212–217 | Cite as

Proanthocyanidins Inhibit Iron Absorption from Soybean (Glycine max) Seed Ferritin in Rats with Iron Deficiency Anemia

  • Shaojun Yun
  • Tuo Zhang
  • Meiliang Li
  • Bin Chen
  • Guanghua Zhao
Original Paper

Abstract

The objective of this study was to evaluate the effect of proanthocyanidins (PAs) on iron uptake from soybean seed ferritin (SSF) crude by rats with iron deficiency anemia (IDA) for the first time. Six groups of Sprague–Dawley (SD) rats (n = 10) were used, which contain (1) SSF crude group; (2) SSF crude + PAs group; (3) PAs group; (4) FeSO4 group; (5) iron deficiency control group; and (6) control group. The bioavailability of iron was examined by measuring hemoglobin (Hb) concentration value, red blood cell (RBC) numbers, and serum iron stores. After 8 weeks, Hb concentration was almost recovered to the normal level upon feeding SSF crude or FeSO4 to rats. In contrast, Hb concentration was recovered to less extent when SSF crude plus PAs was used instead of SSF crude alone (P < 0.05). A similar profile was observed with these three sample groups when serum iron and RBC were used as parameters. All rats in PAs group died at the 8th week. Taken together, all these results demonstrated that PAs inhibited iron uptake of rats from SSF, and are toxic for rats with IDA.

Keywords

Phytoferritin Soybean seed ferritin Proanthocyanidins Association Iron deficiency anemia rats 

Abbreviations

PAs

proanthocyanidins

SSF

soybean seed ferritin

IDA

iron deficiency anemia

SD

Sprague–Dawley

SDS

sodium dodecyl sulfate

Tris

tris (hydroxymethyl) aminomethane

Hb

hemoglobin

RBC

red blood cell

DMT-1

divalent metal transporter −1

Notes

Acknowledgements

This project was supported by State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center (SMFA09A012), the National Science and Technology Support Program (2011BAD23B04), and the Key Laboratory Open Fund of Soybean Biology of Education Ministry.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Shaojun Yun
    • 1
  • Tuo Zhang
    • 1
  • Meiliang Li
    • 1
  • Bin Chen
    • 2
  • Guanghua Zhao
    • 1
  1. 1.CAU & ACC Joint-Laboratory of Space Food, College of Food Science and Nutritional EngineeringChina Agricultural University, Key Laboratory of Functional Dairy, Ministry of Education, Beijing Higher Institution Engineering Research Center of Animal ProductBeijingPeople’s Republic of China
  2. 2.State Key Laboratory of Space Medicine Fundamentals and ApplicationChina Astronaut Research and Training CenterBeijingPeople’s Republic of China

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